LOCUS       sequence-33629-e         903 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 903)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 903)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: zgc:153086_L (OZ511)
FEATURES             Location/Qualifiers
     source          1..903
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             38..61
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      463..891
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(550..569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     760..781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaggccctg agaaaaatcc aagctttggc ggccgctgac tacaaagacg atgatgacaa
       61 atctagaccc ggggagcgcc ccttccagtg tcgcatttgc atgcggaact tttcgaagca
      121 ccagacgctt cagcggcata cccgtactca taccggtgaa aaaccgtttc agtgtcggat
      181 ctgtatgcga aatttctccc tgctgcacaa cttgacgcgg catctacgta cgcacaccgg
      241 cgagaagcca ttccaatgcc gaatatgcat gcgcaacttc agtgtgaacc accacctgga
      301 ccgccaccta aaaacccacc tgaggggatc ctctgcacag tagtaactag agactagaaa
      361 aaggccgaca agtcccgctc cgctgaagat cctggcgtaa tagcgaagag gcccgcaccg
      421 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
      481 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      541 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      601 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      661 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      721 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      781 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      841 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttacaattt
      901 taa
//
