LOCUS       sequence-33631-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, 
            Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, 
            Unger-Wallace E, Sander JD, Muller-Lerch F, Fu F, Pearlberg J, 
            Goebel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate
            AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK
  TITLE     Rapid ""open-source"" engineering of customized zinc-finger 
            nucleases for highly efficient gene modification.
  JOURNAL   Mol Cell. 2008 Jul 25. 31(2):294-301.
  PUBMED    18657511
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2008 Jul 25. 31(2):294-301."
COMMENT     Sequence Label: CACNA1F_R (OZ508)
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             51..74
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     rep_origin      476..834
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(563..582)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     773..794
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ggaatcattg tgcaaaggcc ctgagaaaaa tccaagcttt ggcggccgct gactacaaag
       61 acgatgatga caaatctaga cccggggagc gccccttcca gtgtcgcatt tgcatgcgga
      121 acttttcgaa gaagaccaac cttaccaggc atacccgtac tcataccggt gaaaaaccgt
      181 ttcagtgtcg gatctgtatg cgaaatttct cccagaacgc gaacttggcg cgccatctac
      241 gtacgcacac cggcgagaag ccattccaat gccgaatatg catgcgcaac ttcagtcaga
      301 gggtcgggct ggacatccac ctaaaaaccc acctgagggg atcctctgca cagtagtaac
      361 tagagactag aaaaaggccg acaagtcccg ctccgctgaa gatcctggcg taatagcgaa
      421 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
      481 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
      541 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
      601 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
      661 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
      721 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
      781 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
      841 a
//
