LOCUS       sequence-336387-       10936 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of syt1 in cultured neurons by lentiviral transduction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Bradberry MM, Courtney NA, Dominguez MJ, Lofquist SM, Knox AT, 
            Sutton RB, Chapman ER
  TITLE     Molecular Basis for Synaptotagmin-1-Associated Neurodevelopmental 
            Disorder.
  JOURNAL   Neuron. 2020 Jul 8;107(1):52-64.e7. doi: 
            10.1016/j.neuron.2020.04.003. Epub 2020 May 1.
  PUBMED    32362337
REFERENCE   2  (bases 1 to 10936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2020.04.003"; journalName: "Neuron"; date: 
            "2020-07-8- 8"; volume: "107"; issue: "1"; pages: "52-64.e7"
COMMENT     Sequence Label: pFSynW Syt1 WT IRES mRuby3
FEATURES             Location/Qualifiers
     source          1..10936
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2602..3048
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      3059..3068
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4336..4907
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4357..4909
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4501..4518)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4728..4747
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     misc_feature    5628..6216
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5681..5701)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6099..6110)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6219..6235)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6220..6236)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6741..6921
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6947..6964)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6953..7177
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7223..7651
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7310..7329)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7520..7541
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7660..7680)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7665..7994
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7845..7980
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7907..7926
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8042..8089
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8108..8482
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8612..8733
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8649..8668)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8703..8722
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8782..8798)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8782..8798)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8795..8817)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8806..8822
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8830..8836,8837..8854,8855..8860))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8875..8896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9013..9030)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9184..9772)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9264..9283)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9943..10734,10735..10803))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10566..10585
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10804..10908)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aagtgcaagt ggttttagga ccaggatgag gcggggtggg
     2641 ggtgcctacc tgacgaccga ccccgaccca ctggacaagc acccaacccc cattccccaa
     2701 attgcgcatc ccctatcaga gagggggagg ggaaacagga tgcggcgagg cgcgtgcgca
     2761 ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc ctggcggcgc gcgccaccgc
     2821 cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc ccccgtaaac tccccttccc
     2881 ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg gaccgcacca cgcgaggcgc
     2941 gagatagggg ggcacgggcg cgaccatctg cgctgcggcg ccggcgactc agcgctgcct
     3001 cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga gagcgcaggt acggctaggc
     3061 caccatggtg agtgccagtc atcctgaggc cctggccgcc cctgtcacca ctgttgcgac
     3121 ccttgtccca cacaatgcca ctgagccagc cagtcctggg gaagggaagg aagatgcctt
     3181 ttccaagctg aagcagaagt ttatgaatga gctgcataaa attccattgc caccgtgggc
     3241 cttaatagcc atagccatag ttgcggtcct tttagtcgta acctgctgct tttgtgtctg
     3301 taagaaatgt ttgttcaaaa agaaaaacaa gaagaagggg aaggaaaagg gaggaaagaa
     3361 cgccattaac atgaaagacg tgaaagactt agggaagacc atgaaggatc aggcccttaa
     3421 ggatgacgat gctgaaaccg gactgactga tggagaagaa aaggaagagc ccaaggaaga
     3481 ggagaaactg ggaaagctcc aatattcact ggactatgac ttccagaata accagctgtt
     3541 ggtgggaatc atccaggctg ctgaactgcc cgccctggac atggggggta catccgatcc
     3601 atacgtcaaa gtcttcctgc tgcctgacaa aaagaagaaa tttgagacta aagtccaccg
     3661 gaaaaccctc aatccagtct tcaatgaaca atttactttc aaggtaccct actcggaatt
     3721 aggtggcaaa accctggtga tggctgtgta tgactttgat cgcttctcca agcacgacat
     3781 catcggagag ttcaaagttc ctatgaacac cgtggatttt ggccatgtga ccgaggagtg
     3841 gcgcgatctc cagagcgctg agaaagaaga gcaagagaaa ctgggtgaca tctgcttctc
     3901 cctccgctac gtccctactg ccggcaaact gactgttgtc attctggaag ccaagaacct
     3961 gaagaagatg gatgtgggtg gcttatctga tccctacgtg aagattcacc tgatgcagaa
     4021 cggtaagagg ctgaagaaga aaaagacgac gattaagaag aacacactca acccctacta
     4081 caacgagtcc ttcagctttg aagttccgtt cgagcaaatc cagaaagtgc aagtggtggt
     4141 aactgttttg gactatgaca agattggcaa gaacgacgcc atcggcaaag tcttcgttgg
     4201 ttacaacagc actggggcgg agctgcgaca ctggtcagac atgctggcca acccccggcg
     4261 acccatcgca cagtggcaca ctctgcaggt agaggaggag gtcgatgcca tgctggctgt
     4321 caagaagtaa aattccccct ctccctcccc cccctaacgt tactggccga agccgcttgg
     4381 aataaggccg gtgtgcgttt gtctatatgt tattttccac catattgccg tcttttggca
     4441 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     4501 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     4561 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     4621 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     4681 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     4741 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     4801 tggggcctcg gtacacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     4861 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataatat ggtgtctaag
     4921 ggcgaagagc tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac
     4981 ggccaccaat tcaaatgcac aggtgaagga gaaggcagac cgtacgaggg agtgcaaacc
     5041 atgaggatca aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg
     5101 tcgttcatgt atggcagccg tacctttatc aagtacccgg ccgacatccc tgatttcttt
     5161 aaacagtcct ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga
     5221 gtcgtcaccg tcacgcagga caccagcctt gaggatggcg agctcgtcta caacgtcaag
     5281 gtcagagggg taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg
     5341 gagcctaata cagagatgat gtatccagca gatggtggtc tgagaggata cactgacatc
     5401 gcactgaaag ttgatggtgg tggccatctg cactgcaact tcgtgacaac ttacaggtca
     5461 aaaaagaccg tcgggaacat caagatgccc ggtgtccatg ccgttgatca ccgcctggaa
     5521 aggatcgagg agagtgacaa tgaaacctac gtagtgcaaa gagaagtggc agttgccaaa
     5581 tacagcaacc ttggtggtgg ctaaaattcg atatcaagct tatcgataat caacctctgg
     5641 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5701 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5761 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5821 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     5881 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     5941 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6001 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     6061 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6121 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6181 agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga cctcgagacc
     6241 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     6301 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     6361 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     6421 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     6481 cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc agatatccac
     6541 tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta gaagaagcca
     6601 atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6661 agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac atggcccgag
     6721 agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     6781 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     6841 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     6901 cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc gactgtgcct
     6961 tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt
     7021 gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg
     7081 tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac
     7141 aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagc
     7201 tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     7261 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     7321 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     7381 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     7441 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     7501 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     7561 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     7621 gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt cagttagggt
     7681 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
     7741 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
     7801 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
     7861 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     7921 ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     7981 taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct gatcagcacg
     8041 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa
     8101 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg
     8161 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt
     8221 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt
     8281 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg
     8341 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg
     8401 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt
     8461 cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg ccgccttcta
     8521 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     8581 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     8641 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     8701 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     8761 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8821 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8881 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8941 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     9001 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     9061 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     9121 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     9181 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     9241 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     9301 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     9361 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     9421 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     9481 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     9541 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     9601 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     9661 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     9721 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9781 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9841 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9901 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9961 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
    10021 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
    10081 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
    10141 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
    10201 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
    10261 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
    10321 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
    10381 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
    10441 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
    10501 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
    10561 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
    10621 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
    10681 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
    10741 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10801 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10861 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10921 aaaagtgcca cctgac
//
