LOCUS       sequence-33640-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Cross FR, Pecani K
  TITLE     Efficient and rapid exact gene replacement without selection.
  JOURNAL   Yeast. 2011 Feb . 28(2):167-79.
  PUBMED    21246629
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2011
            Feb . 28(2):167-79."
COMMENT     Sequence Label: FC857
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..24
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6..24
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 cgcgcaatta accctcacta aagggaacaa aagctggagc tccaccgcgg tggcggccgc
       61 aacatctcag ttacccgcac tgcctttttt ctctcccgct tttcagcaga acgaggcgac
      121 aagcgaagga acaaaacaaa acaaaagaaa acaaaaatat cccgggaatc tcggtcgtaa
      181 tgatttttat aatgacgaaa aaaaaaaaat tggaaagaaa aagctttaat gcggtagttt
      241 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
      301 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
      361 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
      421 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
      481 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
      541 gatcatggcg accacacccg tcctgtggat cttatattct ccagattcca aagctagaaa
      601 gctgaagtca ccggaaggag agccctgttg gtggactacc ctatgattat tatcaaagac
      661 ttcttccaca tctatgacaa tgccatagtc attataactg ggaagcgcat aatctgccaa
      721 cttatcatcg taaacttcca aattgtaaga accttttaaa agggtgtctt tggatagttc
      781 cttcaggaag cattttctgt ctccaccata accatagaag taaaacgcgg aaactctgaa
      841 tggcaataaa acgaatgcca ggaagatgta gtgtgataac attggcaaat ctagatcctt
      901 tcagattga
//
