LOCUS       sequence-336410-        2973 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Huang D, Tran JT, Peng L, Yang L, Suhandynata RT, Hoffman MA, Zhao 
            F, Song G, He WT, Limbo O, Callaghan S, Landais E, Andrabi R, Sok D,
            Jardine JG, Burton DR, Voss JE, Fitzgerald RL, Nemazee D
  TITLE     A Rapid Assay for SARS-CoV-2 Neutralizing Antibodies That Is 
            Insensitive to Antiretroviral Drugs.
  JOURNAL   J Immunol. 2021 Jul 1;207(1):344-351. doi: 10.4049/jimmunol.2100155.
            Epub 2021 Jun 28.
  PUBMED    34183368
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.4049/jimmunol.2100155"; journalName: "J Immunol"; date: 
            "2021-07-1- 1"; volume: "207"; issue: "1"; pages: "344-351"
COMMENT     Sequence Label: 2XSmBiT-SARS-CoV-2 S1
FEATURES             Location/Qualifiers
     source          1..2973
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1535..1552)
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
ORIGIN
        1 atggaaaccg atacactgct gctgtgggtg ctgttgctct gggttccagg ctctaccggc
       61 gtgaccggct accggctgtt cgaggagatt ctcggaagtt ccggtggagg agggagcgga
      121 ggtggaggat cgtctggagg aggaggtgga gggtctagcg gtgtgaccgg ctaccggctg
      181 ttcgaggaga ttctcggaag ttccggtgga ggagggagcg gaggtggagg atcgtctgga
      241 ggaggaggtg gagggtctag ccagtgtgtg aacctgacca ccaggaccca acttcctcct
      301 gcctacacca actccttcac caggggagtc tactaccctg acaaggtgtt caggtcctct
      361 gtgctgcaca gcacccagga cctgttcctg ccattcttca gcaatgtgac ctggttccat
      421 gccatccatg tgtctggcac caatggcacc aagaggtttg acaaccctgt gctgccattc
      481 aatgatggag tctactttgc cagcacagag aagagcaaca tcatcagggg ctggattttt
      541 ggcaccaccc tggacagcaa gacccagtcc ctgctgattg tgaacaatgc caccaatgtg
      601 gtgattaagg tgtgtgagtt ccagttctgt aatgacccat tcctgggagt ctactaccac
      661 aagaacaaca agtcctggat ggagtctgag ttcagggtct actcctctgc caacaactgt
      721 acctttgaat atgtgagcca accattcctg atggacttgg agggcaagca gggcaacttc
      781 aagaacctga gggagtttgt gttcaagaac attgatggct acttcaagat ttacagcaaa
      841 cacacaccaa tcaacctggt gagggacctg ccacagggct tctctgcctt ggaaccactg
      901 gtggacctgc caattggcat caacatcacc aggttccaga ccctgctggc tctgcacagg
      961 tcctacctga cacctggaga ctcctcctct ggctggacag caggagcagc agcctactat
     1021 gtgggctacc tccaaccaag gaccttcctg ctgaaataca atgagaatgg caccatcaca
     1081 gatgctgtgg actgtgccct ggacccactg tctgagacca agtgtaccct gaaatccttc
     1141 acagtggaga agggcatcta ccagaccagc aacttcaggg tccaaccaac agagagcatt
     1201 gtgaggtttc caaacatcac caacctgtgt ccatttggag aggtgttcaa tgccaccagg
     1261 tttgcctctg tctatgcctg gaacaggaag aggattagca actgtgtggc tgactactct
     1321 gtgctctaca actctgcctc cttcagcacc ttcaagtgtt atggagtgag cccaaccaaa
     1381 ctgaatgacc tgtgtttcac caatgtctat gctgactcct ttgtgattag gggagatgag
     1441 gtgagacaga ttgcccctgg acaaacaggc aagattgctg actacaacta caaactgcct
     1501 gatgacttca caggctgtgt gattgcctgg aacagcaaca acctggacag caaggtggga
     1561 ggcaactaca actacctcta cagactgttc aggaagagca acctgaaacc atttgagagg
     1621 gacatcagca cagagattta ccaggctggc agcacaccat gtaatggagt ggagggcttc
     1681 aactgttact ttccactcca atcctatggc ttccaaccaa ccaatggagt gggctaccaa
     1741 ccatacaggg tggtggtgct gtcctttgaa ctgctccatg cccctgccac agtgtgtgga
     1801 ccaaagaaga gcaccaacct ggtgaagaac aagtgtgtga acttcaactt caatggactg
     1861 acaggcacag gagtgctgac agagagcaac aagaagttcc tgccattcca acagtttggc
     1921 agggacattg ctgacaccac agatgctgtg agggacccac agaccttgga gattctggac
     1981 atcacaccat gttcctttgg aggagtgtct gtgattacac ctggcaccaa caccagcaac
     2041 caggtggctg tgctctacca ggatgtgaac tgtactgagg tgcctgtggc tatccatgct
     2101 gaccaactta caccaacctg gagggtctac agcacaggca gcaatgtgtt ccagaccagg
     2161 gctggctgtc tgattggagc agagcatgtg aacaactcct atgagtgtga catcccaatt
     2221 ggagcaggca tctgtgcctc ctaccagacc cagaccaaca gcccaggcag cgccagcccc
     2281 aagagctgcg acaagaccca cacctgtcct ccatgtcctg ctccagaact gctcggcgga
     2341 ccttccgtgt tcctgtttcc tccaaagcct aaggacaccc tgatgatcag cagaacccct
     2401 gaagtgacct gcgtggtggt ggatgtgtcc cacgaggatc ccgaagtgaa gttcaattgg
     2461 tacgtggacg gcgtggaagt gcacaacgcc aagaccaagc ctagagagga acagtacaac
     2521 agcacctaca gagtggtgtc cgtgctgacc gtgctgcacc aggattggct gaacggcaaa
     2581 gagtacaagt gcaaggtgtc caacaaggcc ctgcctgctc ctatcgagaa aaccatcagc
     2641 aaggccaagg gccagcctag ggaaccccag gtttacacac tgcctccaag cagggacgag
     2701 ctgaccaaga atcaggtgtc cctgacctgc ctggtcaagg gcttctaccc ttccgatatc
     2761 gccgtggaat gggagagcaa tggccagcct gagaacaact acaagacaac ccctcctgtg
     2821 ctggacagcg acggctcatt cttcctgtac agcaagctga cagtggacaa gagcagatgg
     2881 cagcagggca acgtgttcag ctgcagcgtg atgcacgagg ccctgcacaa ccactacacc
     2941 cagaagtccc tgagcctgtc tcctggcaaa tga
//
