LOCUS       sequence-336425-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 D110V mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_D110V_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(74..94)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        79..408
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      259..394
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        456..503
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             522..896
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1026..1147
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1063..1082)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1117..1136
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1196..1212)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1196..1212)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1209..1231)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1220..1236
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1244..1250,1251..1268,1269..1274))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1289..1310
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1427..1444)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1598..2186)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1678..1697)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2357..3148,3149..3217))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2980..2999
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3218..3322)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3397..3416)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3588..3967
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3969..4167
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4122..4142
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4185..4365
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4412..4537
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5030..5263
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5448..5492
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5790..5907
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6043..7221
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6274..7212
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7169..7189
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7238..7257
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7238..7256
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7320..8342
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7320..8342
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8352..8408
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9962..9995)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10020..10200
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10226..10243)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10232..10456
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10502..10865,1..65)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10589..10608)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10799..10820
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
       61 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
      121 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
      181 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
      241 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
      301 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
      361 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
      421 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
      481 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
      541 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
      601 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
      661 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
      721 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
      781 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
      841 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
      901 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
      961 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
     1021 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     1081 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     1141 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     1201 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     1261 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     1321 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     1381 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     1441 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     1501 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     1561 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     1621 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     1681 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     1741 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     1801 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     1861 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     1921 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     1981 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     2041 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     2101 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     2161 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     2221 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     2281 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     2341 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     2401 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     2461 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     2521 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     2581 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     2641 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     2701 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     2761 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     2821 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     2881 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     2941 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     3001 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     3061 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     3121 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     3181 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     3241 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     3301 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat
     3361 cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg atgccgcata
     3421 gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt gcgcgagcaa
     3481 aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc tgcttagggt
     3541 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
     3601 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
     3661 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     3721 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     3781 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     3841 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     3901 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     3961 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     4021 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     4081 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     4141 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
     4201 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4261 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4321 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     4381 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     4441 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     4501 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     4561 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     4621 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     4681 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     4741 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     4801 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     4861 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     4921 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     4981 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     5041 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     5101 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     5161 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     5221 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     5281 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     5341 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     5401 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     5461 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     5521 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     5581 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     5641 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     5701 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     5761 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     5821 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     5881 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     5941 tggttaatta atcgataagc tttgcaaaga tggataaagt tttaaacaga gaggaatctt
     6001 tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag
     6061 tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt cggcaattga
     6121 accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt gtactggctc
     6181 cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt
     6241 ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg ttcccgcggg
     6301 cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac ctggctgcag
     6361 tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc gaggccttgc
     6421 gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg ctggggccgc
     6481 cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa gtctctagcc
     6541 atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag tcttgtaaat
     6601 gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg cgacggggcc
     6661 cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc accgagaatc
     6721 ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc gccgccgtgt
     6781 atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg agcggaaaga
     6841 tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg ctcgggagag
     6901 cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc cgtcgcttca
     6961 tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc gagcttttgg
     7021 agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc ccacactgag
     7081 tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt ggaatttgcc
     7141 ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca aagttttttt
     7201 cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac tatagggaga
     7261 cccaagctgg ctaggtaagc ttgatatcga attcctgcag cccgggggat cccgccacca
     7321 tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa aagttcgaca
     7381 gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc agcttcgatg
     7441 taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc tacaaagatc
     7501 gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg cttgacattg
     7561 gggaattcag cgagagcctg acctattgca tctcccgccg tgcacagggt gtcacgttgc
     7621 aagacctgcc tgaaaccgaa ctgcccgctg ttctgcagcc ggtcgcggag gccatggatg
     7681 cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga ccgcaaggaa
     7741 tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc catgtgtatc
     7801 actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct ctcgatgagc
     7861 tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg gatttcggct
     7921 ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg agcgaggcga
     7981 tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg tggttggctt
     8041 gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca ggatcgccgc
     8101 ggctccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc ttggttgacg
     8161 gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc cgatccggag
     8221 ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg accgatggct
     8281 gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg agggcaaagg
     8341 aaggatccgg cgcaacaaac ttctctctgc tgaaacaagc cggagatgtc gaagagaatc
     8401 ctggaccgat gcgtatcccc gtagatgcca gcacgagccg ccgcttcacg ccgccttcca
     8461 ccgcgctgag cccaggcaag atgagcgagg cgttgccgct gggcgccccg gacgccggcg
     8521 ctgccctggc cggcaagctg aggagcggcg accgcagcat ggtggaggtg ctggccgacc
     8581 acccgggcga gctggtgcgc accgacagcc ccaacttcct ctgctccgtg ctgcctacgc
     8641 actggcgctg caacaagacc ctgcccatcg ctttcaaggt ggtggcccta ggggatgttc
     8701 cagatggcac tctggtcact gtgatggctg gcaatgttga aaactactcg gctgagctga
     8761 gaaatgctac cgcagccatg aagaaccagg ttgcaagatt taatgacctc aggtttgtcg
     8821 gtcgaagtgg aagagggaaa agcttcactc tgaccatcac tgtcttcaca aacccaccgc
     8881 aagtcgccac ctaccacaga gccatcaaaa tcacagtgga tgggccccga gaacctcgaa
     8941 gacatcggca gaaactagat gatcagacca agcccgggag cttgtccttt tccgagcggc
     9001 tcagtgaact ggagcagctg cggcgcacag ccatgagggt cagcccacac cacccagccc
     9061 ccacgcccaa ccctcgtgcc tccctgaacc actccactgc ctttaaccct cagcctcaga
     9121 gtcagatgca ggatacaagg cagatccaac catccccacc gtggtcctac gatcagtcct
     9181 accaatacct gggatccatt gcctctcctt ctgtgcaccc agcaacgccc atttcacctg
     9241 gacgtgccag cggcatgaca accctctctg cagaactttc cagtcgactc tcaacggcac
     9301 ccgacctgac agcgttcagc gacccgcgcc agttccccgc gctgccctcc atctccgacc
     9361 cccgcatgca ctatccaggc gccttcacct actccccgac gccggtcacc tcgggcatcg
     9421 gcatcggcat gtcggccatg ggctcggcca cgcgctacca cacctacctg ccgccgccct
     9481 accccggctc gtcgcaagcg cagggaggcc cgttccaagc cagctcgccc tcctaccacc
     9541 tgtactacgg cgcctcggcc ggctcctacc agttctccat ggtgggcggc gagcgctcgc
     9601 cgccgcgcat cctgccgccc tgcaccaacg cctccaccgg ctccgcgctg ctcaacccca
     9661 gcctcccgaa ccagagcgac gtggtggagg ccgagggcag ccacagcaac tcccccacca
     9721 acatggcgcc ctccgcgcgc ctggaggagg ccgtgtggag gccctactga ctagctaagt
     9781 tagaactatt tcctggctgt tacgcgtagt tcggggtact actgacgggc ttgttaaaca
     9841 gtgaaccgcc cgccccacga cccgcagcgc ccgaccgaaa ggagcgcacg accccatgca
     9901 tcgaacaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     9961 cataacttcg tatagcatac attatacgaa gttatcgtct agctggtccg gactgtactg
    10021 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
    10081 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
    10141 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
    10201 gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
    10261 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
    10321 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
    10381 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
    10441 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc
    10501 cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
    10561 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
    10621 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
    10681 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
    10741 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
    10801 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
    10861 taagg
//
