LOCUS       sequence-336427-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 G100V mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_G100V_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..51
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             70..444
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    574..695
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(611..630)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     665..684
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(744..760)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(744..760)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(757..779)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    768..784
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(792..798,799..816,817..822))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    837..858
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(975..992)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1146..1734)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1226..1245)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1905..2696,2697..2765))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2528..2547
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2766..2870)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(2945..2964)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3136..3515
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3517..3715
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3670..3690
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             3733..3913
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3960..4085
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4578..4811
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4996..5040
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5338..5455
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5591..6769
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5822..6760
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6717..6737
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6786..6805
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        6786..6804
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             6868..7890
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6868..7890
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7900..7956
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9510..9543)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9568..9748
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9774..9791)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9780..10004
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10050..10478
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10137..10156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10347..10368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10487..10507)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10492..10821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10672..10807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
       61 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
      121 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
      181 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
      241 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
      301 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
      361 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
      421 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
      481 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
      541 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
      601 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
      661 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
      721 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
      781 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
      841 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
      901 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
      961 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     1021 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     1081 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     1141 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     1201 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     1261 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     1321 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     1381 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     1441 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     1501 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     1561 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     1621 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     1681 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     1741 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     1801 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     1861 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     1921 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1981 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     2041 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2101 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     2161 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     2221 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2281 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2341 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2401 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     2461 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2521 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     2581 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2641 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2701 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2761 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     2821 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     2881 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     2941 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     3001 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     3061 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     3121 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
     3181 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     3241 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     3301 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     3361 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     3421 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     3481 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
     3541 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
     3601 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
     3661 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagcgcgt
     3721 tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     3781 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     3841 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     3901 aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc
     3961 tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac
     4021 tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga
     4081 gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag
     4141 ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat
     4201 tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc
     4261 tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa
     4321 ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga
     4381 tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct gatcttcaga
     4441 cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta
     4501 aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa
     4561 aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact
     4621 atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg
     4681 cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca
     4741 gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat
     4801 caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct
     4861 tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg
     4921 gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg
     4981 caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg
     5041 tggaattggt ttaacataac aaattggctg tggtatataa aattattcat aatgatagta
     5101 ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg
     5161 cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg
     5221 cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg
     5281 aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca tccacaattt
     5341 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
     5401 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttcgggt
     5461 ttattacagg gacagcagag atccagtttg gttaattaat cgataagctt tgcaaagatg
     5521 gataaagttt taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg
     5581 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     5641 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     5701 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     5761 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     5821 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     5881 gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg
     5941 aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt
     6001 tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg
     6061 tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct
     6121 ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt
     6181 ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg
     6241 gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc
     6301 tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg
     6361 gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc
     6421 aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag
     6481 ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag
     6541 gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt
     6601 ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca
     6661 cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa
     6721 gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattagctt
     6781 ggtactaata cgactcacta tagggagacc caagctggct aggtaagctt gatatcgaat
     6841 tcctgcagcc cgggggatcc cgccaccatg aaaaagcctg aactcaccgc gacgtctgtc
     6901 gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct ctcggagggc
     6961 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     7021 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     7081 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     7141 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     7201 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     7261 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     7321 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     7381 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     7441 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     7501 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     7561 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     7621 aggcatccgg agcttgcagg atcgccgcgg ctccgggcgt atatgctccg cattggtctt
     7681 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     7741 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     7801 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     7861 cgccccagca ctcgtccgag ggcaaaggaa ggatccggcg caacaaactt ctctctgctg
     7921 aaacaagccg gagatgtcga agagaatcct ggaccgatgc gtatccccgt agatgccagc
     7981 acgagccgcc gcttcacgcc gccttccacc gcgctgagcc caggcaagat gagcgaggcg
     8041 ttgccgctgg gcgccccgga cgccggcgct gccctggccg gcaagctgag gagcggcgac
     8101 cgcagcatgg tggaggtgct ggccgaccac ccgggcgagc tggtgcgcac cgacagcccc
     8161 aacttcctct gctccgtgct gcctacgcac tggcgctgca acaagaccct gcccatcgct
     8221 ttcaaggtgg tggccctagg ggatgttcca gatgtcactc tggtcactgt gatggctggc
     8281 aatgatgaaa actactcggc tgagctgaga aatgctaccg cagccatgaa gaaccaggtt
     8341 gcaagattta atgacctcag gtttgtcggt cgaagtggaa gagggaaaag cttcactctg
     8401 accatcactg tcttcacaaa cccaccgcaa gtcgccacct accacagagc catcaaaatc
     8461 acagtggatg ggccccgaga acctcgaaga catcggcaga aactagatga tcagaccaag
     8521 cccgggagct tgtccttttc cgagcggctc agtgaactgg agcagctgcg gcgcacagcc
     8581 atgagggtca gcccacacca cccagccccc acgcccaacc ctcgtgcctc cctgaaccac
     8641 tccactgcct ttaaccctca gcctcagagt cagatgcagg atacaaggca gatccaacca
     8701 tccccaccgt ggtcctacga tcagtcctac caatacctgg gatccattgc ctctccttct
     8761 gtgcacccag caacgcccat ttcacctgga cgtgccagcg gcatgacaac cctctctgca
     8821 gaactttcca gtcgactctc aacggcaccc gacctgacag cgttcagcga cccgcgccag
     8881 ttccccgcgc tgccctccat ctccgacccc cgcatgcact atccaggcgc cttcacctac
     8941 tccccgacgc cggtcacctc gggcatcggc atcggcatgt cggccatggg ctcggccacg
     9001 cgctaccaca cctacctgcc gccgccctac cccggctcgt cgcaagcgca gggaggcccg
     9061 ttccaagcca gctcgccctc ctaccacctg tactacggcg cctcggccgg ctcctaccag
     9121 ttctccatgg tgggcggcga gcgctcgccg ccgcgcatcc tgccgccctg caccaacgcc
     9181 tccaccggct ccgcgctgct caaccccagc ctcccgaacc agagcgacgt ggtggaggcc
     9241 gagggcagcc acagcaactc ccccaccaac atggcgccct ccgcgcgcct ggaggaggcc
     9301 gtgtggaggc cctactgact agctaagtta gaactatttc ctggctgtta cgcggttcca
     9361 tatgacctac tgacgggctt gttaaacagt gaaccgcccg ccccacgacc cgcagcgccc
     9421 gaccgaaagg agcgcacgac cccatgcatc gaacaaaaga aaagggggga ctggaagggc
     9481 taattcactc ccaacgaaga caagatatca taacttcgta tagcatacat tatacgaagt
     9541 tatcgtctag ctggtccgga ctgtactggg tctctctggt tagaccagat ctgagcctgg
     9601 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9661 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9721 ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat cagcctcgac
     9781 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
     9841 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
     9901 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
     9961 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
    10021 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taagcgcggc
    10081 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    10141 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    10201 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    10261 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    10321 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    10381 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    10441 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
    10501 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    10561 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10621 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10741 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
    10801 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
    10861 cagca
//
