LOCUS       sequence-336429-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 V105E mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_V105E_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(45..65)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        50..379
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      230..365
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        427..474
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             493..867
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    997..1118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1034..1053)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1088..1107
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1167..1183)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1167..1183)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1180..1202)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1191..1207
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1215..1221,1222..1239,1240..1245))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1260..1281
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1398..1415)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1569..2157)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1649..1668)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2328..3119,3120..3188))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2951..2970
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3189..3293)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3368..3387)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3559..3938
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3940..4138
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4093..4113
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4156..4336
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4383..4508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5001..5234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5419..5463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5761..5878
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6014..7192
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6245..7183
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7140..7160
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7209..7228
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7209..7227
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7291..8313
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7291..8313
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8323..8379
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9933..9966)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             9991..10171
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10197..10214)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10203..10427
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10473..10865,1..36)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10560..10579)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10770..10791
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
       61 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      121 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      181 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      241 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      301 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
      361 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
      421 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
      481 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
      541 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
      601 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
      661 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
      721 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
      781 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
      841 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
      901 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
      961 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     1021 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     1081 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     1141 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     1201 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     1261 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     1321 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     1381 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     1441 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     1501 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     1561 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     1621 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1681 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1741 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1801 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1861 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1921 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1981 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     2041 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     2101 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     2161 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     2221 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     2281 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     2341 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     2401 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     2461 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     2521 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     2581 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     2641 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2701 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2761 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2821 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2881 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2941 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     3001 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     3061 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     3121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     3181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     3241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     3301 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
     3361 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
     3421 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
     3481 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
     3541 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
     3601 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     3661 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     3721 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     3781 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     3841 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     3901 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     3961 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     4021 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     4081 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     4141 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     4201 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     4261 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     4321 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     4381 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     4441 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     4501 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     4561 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     4621 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     4681 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     4741 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     4801 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     4861 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     4921 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     4981 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     5041 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     5101 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     5161 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     5221 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     5281 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     5341 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     5401 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     5461 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     5521 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     5581 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     5641 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     5701 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
     5761 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
     5821 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
     5881 ggtttattac agggacagca gagatccagt ttggttaatt aatcgataag ctttgcaaag
     5941 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     6001 aggagtggga attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc
     6061 cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt
     6121 aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc
     6181 gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac
     6241 acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg
     6301 cgtgccttga attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     6361 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     6421 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     6481 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     6541 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     6601 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     6661 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     6721 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     6781 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     6841 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     6901 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     6961 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     7021 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     7081 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     7141 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag
     7201 cttggtacta atacgactca ctatagggag acccaagctg gctaggtaag cttgatatcg
     7261 aattcctgca gcccggggga tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct
     7321 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     7381 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     7441 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     7501 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     7561 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     7621 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     7681 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     7741 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     7801 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     7861 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     7921 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     7981 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     8041 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     8101 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     8161 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     8221 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     8281 cgacgcccca gcactcgtcc gagggcaaag gaaggatccg gcgcaacaaa cttctctctg
     8341 ctgaaacaag ccggagatgt cgaagagaat cctggaccga tgcgtatccc cgtagatgcc
     8401 agcacgagcc gccgcttcac gccgccttcc accgcgctga gcccaggcaa gatgagcgag
     8461 gcgttgccgc tgggcgcccc ggacgccggc gctgccctgg ccggcaagct gaggagcggc
     8521 gaccgcagca tggtggaggt gctggccgac cacccgggcg agctggtgcg caccgacagc
     8581 cccaacttcc tctgctccgt gctgcctacg cactggcgct gcaacaagac cctgcccatc
     8641 gctttcaagg tggtggccct aggggatgtt ccagatggca ctctggtcac tgagatggct
     8701 ggcaatgatg aaaactactc ggctgagctg agaaatgcta ccgcagccat gaagaaccag
     8761 gttgcaagat ttaatgacct caggtttgtc ggtcgaagtg gaagagggaa aagcttcact
     8821 ctgaccatca ctgtcttcac aaacccaccg caagtcgcca cctaccacag agccatcaaa
     8881 atcacagtgg atgggccccg agaacctcga agacatcggc agaaactaga tgatcagacc
     8941 aagcccggga gcttgtcctt ttccgagcgg ctcagtgaac tggagcagct gcggcgcaca
     9001 gccatgaggg tcagcccaca ccacccagcc cccacgccca accctcgtgc ctccctgaac
     9061 cactccactg cctttaaccc tcagcctcag agtcagatgc aggatacaag gcagatccaa
     9121 ccatccccac cgtggtccta cgatcagtcc taccaatacc tgggatccat tgcctctcct
     9181 tctgtgcacc cagcaacgcc catttcacct ggacgtgcca gcggcatgac aaccctctct
     9241 gcagaacttt ccagtcgact ctcaacggca cccgacctga cagcgttcag cgacccgcgc
     9301 cagttccccg cgctgccctc catctccgac ccccgcatgc actatccagg cgccttcacc
     9361 tactccccga cgccggtcac ctcgggcatc ggcatcggca tgtcggccat gggctcggcc
     9421 acgcgctacc acacctacct gccgccgccc taccccggct cgtcgcaagc gcagggaggc
     9481 ccgttccaag ccagctcgcc ctcctaccac ctgtactacg gcgcctcggc cggctcctac
     9541 cagttctcca tggtgggcgg cgagcgctcg ccgccgcgca tcctgccgcc ctgcaccaac
     9601 gcctccaccg gctccgcgct gctcaacccc agcctcccga accagagcga cgtggtggag
     9661 gccgagggca gccacagcaa ctcccccacc aacatggcgc cctccgcgcg cctggaggag
     9721 gccgtgtgga ggccctactg actagctaag ttagaactat ttcctggctg ttacgcgtcg
     9781 cagtaatcac tactgacggg cttgttaaac agtgaaccgc ccgccccacg acccacagcg
     9841 cccgaccgaa aggagcgcac gaccccatgc atcgaacaaa agaaaagggg ggactggaag
     9901 ggctaattca ctcccaacga agacaagata tcataacttc gtatagcata cattatacga
     9961 agttatcgtc tagctggtcc ggactgtact gggtctctct ggttagacca gatctgagcc
    10021 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
    10081 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
    10141 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
    10201 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
    10261 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
    10321 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
    10381 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
    10441 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
    10501 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
    10561 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
    10621 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
    10681 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
    10741 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
    10801 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
    10861 gttaa
//
