LOCUS       sequence-336430-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 C81Y mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_C81Y_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(58..78)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        63..392
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      243..378
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        440..487
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             506..880
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1010..1131
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1047..1066)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1101..1120
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1180..1196)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1180..1196)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1193..1215)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1204..1220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1228..1234,1235..1252,1253..1258))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1273..1294
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1411..1428)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1582..2170)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1662..1681)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2341..3132,3133..3201))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2964..2983
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3202..3306)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3381..3400)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3572..3951
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3953..4151
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4106..4126
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4169..4349
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4396..4521
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5014..5247
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5432..5476
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5774..5891
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6027..7205
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6258..7196
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7153..7173
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7222..7241
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7222..7240
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7304..8326
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7304..8326
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8336..8392
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9946..9979)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10004..10184
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10210..10227)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10216..10440
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10486..10865,1..49)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10573..10592)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10783..10804
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga
       61 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
      121 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
      181 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
      241 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
      301 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
      361 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
      421 tcggatctga tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata
      481 atacgacaag gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac
      541 cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga
      601 cttcgtggag gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc
      661 ggtccaggac caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga
      721 cgagctgtac gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc
      781 ggccatgacc gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc
      841 cggcaactgc gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga
      901 ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg
      961 gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat
     1021 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     1081 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
     1141 tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg
     1201 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
     1261 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
     1321 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
     1381 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     1441 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     1501 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     1561 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     1621 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     1681 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     1741 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     1801 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     1861 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     1921 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     1981 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
     2041 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     2101 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     2161 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     2221 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     2281 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     2341 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     2401 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     2461 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     2521 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     2581 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     2641 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     2701 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     2761 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     2821 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     2881 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     2941 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     3001 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     3061 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     3121 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     3181 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     3241 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     3301 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atctcccgat
     3361 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     3421 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     3481 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     3541 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     3601 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     3661 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     3721 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     3781 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
     3841 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
     3901 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     3961 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     4021 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     4081 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     4141 tatataagca gcgcgttttg cctgtactgg gtctctctgg ttagaccaga tctgagcctg
     4201 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4261 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4321 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4381 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4441 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4501 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4561 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4621 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4681 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4741 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4801 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4861 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     4921 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     4981 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     5041 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5101 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5161 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5221 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5281 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5341 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5401 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5461 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5521 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5581 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5641 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5701 atccattcga ttagtgaacg gatcggcact gcgtgcgcca attctgcaga caaatggcag
     5761 tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa
     5821 tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa
     5881 ttcaaaattt tcgggtttat tacagggaca gcagagatcc agtttggtta attaatcgat
     5941 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     6001 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     6061 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     6121 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     6181 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     6241 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     6301 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     6361 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     6421 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     6481 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     6541 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     6601 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     6661 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     6721 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     6781 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     6841 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     6901 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     6961 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     7021 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     7081 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     7141 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     7201 cgtgaggaat tagcttggta ctaatacgac tcactatagg gagacccaag ctggctaggt
     7261 aagcttgata tcgaattcct gcagcccggg ggatcccgcc accatgaaaa agcctgaact
     7321 caccgcgacg tctgtcgaga agtttctgat cgaaaagttc gacagcgtct ccgacctgat
     7381 gcagctctcg gagggcgaag aatctcgtgc tttcagcttc gatgtaggag ggcgtggata
     7441 tgtcctgcgg gtaaatagct gcgccgatgg tttctacaaa gatcgttatg tttatcggca
     7501 ctttgcatcg gccgcgctcc cgattccgga agtgcttgac attggggaat tcagcgagag
     7561 cctgacctat tgcatctccc gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac
     7621 cgaactgccc gctgttctgc agccggtcgc ggaggccatg gatgcgatcg ctgcggccga
     7681 tcttagccag acgagcgggt tcggcccatt cggaccgcaa ggaatcggtc aatacactac
     7741 atggcgtgat ttcatatgcg cgattgctga tccccatgtg tatcactggc aaactgtgat
     7801 ggacgacacc gtcagtgcgt ccgtcgcgca ggctctcgat gagctgatgc tttgggccga
     7861 ggactgcccc gaagtccggc acctcgtgca cgcggatttc ggctccaaca atgtcctgac
     7921 ggacaatggc cgcataacag cggtcattga ctggagcgag gcgatgttcg gggattccca
     7981 atacgaggtc gccaacatct tcttctggag gccgtggttg gcttgtatgg agcagcagac
     8041 gcgctacttc gagcggaggc atccggagct tgcaggatcg ccgcggctcc gggcgtatat
     8101 gctccgcatt ggtcttgacc aactctatca gagcttggtt gacggcaatt tcgatgatgc
     8161 agcttgggcg cagggtcgat gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg
     8221 tacacaaatc gcccgcagaa gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc
     8281 cgatagtgga aaccgacgcc ccagcactcg tccgagggca aaggaaggat ccggcgcaac
     8341 aaacttctct ctgctgaaac aagccggaga tgtcgaagag aatcctggac cgatgcgtat
     8401 ccccgtagat gccagcacga gccgccgctt cacgccgcct tccaccgcgc tgagcccagg
     8461 caagatgagc gaggcgttgc cgctgggcgc cccggacgcc ggcgctgccc tggccggcaa
     8521 gctgaggagc ggcgaccgca gcatggtgga ggtgctggcc gaccacccgg gcgagctggt
     8581 gcgcaccgac agccccaact tcctctgctc cgtgctgcct acgcactggc gctacaacaa
     8641 gaccctgccc atcgctttca aggtggtggc cctaggggat gttccagatg gcactctggt
     8701 cactgtgatg gctggcaatg atgaaaacta ctcggctgag ctgagaaatg ctaccgcagc
     8761 catgaagaac caggttgcaa gatttaatga cctcaggttt gtcggtcgaa gtggaagagg
     8821 gaaaagcttc actctgacca tcactgtctt cacaaaccca ccgcaagtcg ccacctacca
     8881 cagagccatc aaaatcacag tggatgggcc ccgagaacct cgaagacatc ggcagaaact
     8941 agatgatcag accaagcccg ggagcttgtc cttttccgag cggctcagtg aactggagca
     9001 gctgcggcgc acagccatga gggtcagccc acaccaccca gcccccacgc ccaaccctcg
     9061 tgcctccctg aaccactcca ctgcctttaa ccctcagcct cagagtcaga tgcaggatac
     9121 aaggcagatc caaccatccc caccgtggtc ctacgatcag tcctaccaat acctgggatc
     9181 cattgcctct ccttctgtgc acccagcaac gcccatttca cctggacgtg ccagcggcat
     9241 gacaaccctc tctgcagaac tttccagtcg actctcaacg gcacccgacc tgacagcgtt
     9301 cagcgacccg cgccagttcc ccgcgctgcc ctccatctcc gacccccgca tgcactatcc
     9361 aggcgccttc acctactccc cgacgccggt cacctcgggc atcggcatcg gcatgtcggc
     9421 catgggctcg gccacgcgct accacaccta cctgccgccg ccctaccccg gctcgtcgca
     9481 agcgcaggga ggcccgttcc aagccagctc gccctcctac cacctgtact acggcgcctc
     9541 ggccggctcc taccagttct ccatggtggg cggcgagcgc tcgccgccgc gcatcctgcc
     9601 gccctgcacc aacgcctcca ccggctccgc gctgctcaac cccagcctcc cgaaccagag
     9661 cgacgtggtg gaggccgagg gcagccacag caactccccc accaacatgg cgccctccgc
     9721 gcgcctggag gaggccgtgt ggaggcccta ctgactagct aagttagaac tatttcctgg
     9781 ctgttacgcg attgtcatgg gactactgac gggcttgtta aacagtgaac cgcccgcccc
     9841 acgacccgca gcgcccgacc gaaaggagcg cacgacccca tgcatcgaac aaaagaaaag
     9901 gggggactgg aagggctaat tcactcccaa cgaagacaag atatcataac ttcgtatagc
     9961 atacattata cgaagttatc gtctagctgg tccggactgt actgggtctc tctggttaga
    10021 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    10081 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    10141 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc
    10201 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
    10261 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
    10321 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
    10381 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
    10441 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
    10501 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
    10561 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
    10621 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
    10681 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
    10741 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
    10801 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
    10861 tttcg
//
