LOCUS       sequence-336432-       10864 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 S67R mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10864)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_S67R_Barcode
FEATURES             Location/Qualifiers
     source          1..10864
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(138..171)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             196..376
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(402..419)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    408..632
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      678..1106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(765..784)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     975..996
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1115..1135)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1120..1449
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1300..1435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        1497..1544
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1563..1937
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2067..2188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2104..2123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2158..2177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2237..2253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2237..2253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2250..2272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2261..2277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2285..2291,2292..2309,2310..2315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2330..2351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2468..2485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2639..3227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2719..2738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3398..4189,4190..4258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4021..4040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4259..4363)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4438..4457)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4629..5008
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5010..5208
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5163..5183
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5226..5406
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5453..5578
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6071..6304
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6489..6533
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6831..6948
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7084..8262
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7315..8253
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8210..8230
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     8279..8298
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8279..8297
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8361..9383
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8361..9383
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             9393..9449
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 gacgggcttg ttaaacagtg aaccgcccgc cccacgaccg cagcgcccga ccgaaaggag
       61 cgcacgaccc catgcatcga acaaaagaaa aggggggact ggaagggcta attcactccc
      121 aacgaagaca agatatcata acttcgtata gcatacatta tacgaagtta tcgtctagct
      181 ggtccggact gtactgggtc tctatggtta gaccagatct gagcctggga gctctctggc
      241 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
      301 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
      361 tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag
      421 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      481 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      541 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      601 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg
      661 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
      721 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
      781 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
      841 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
      901 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
      961 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     1021 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     1081 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga
     1141 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     1201 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     1261 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     1321 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     1381 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     1441 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca gcacgtgttg
     1501 acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg aggaactaaa
     1561 ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg
     1621 tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg
     1681 gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg
     1741 acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg
     1801 aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc
     1861 agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg
     1921 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa
     1981 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     2041 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     2101 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2161 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga
     2221 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2281 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     2341 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     2401 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
     2461 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     2521 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     2581 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     2641 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     2701 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     2761 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     2821 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     2881 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     2941 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     3001 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     3061 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
     3121 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     3181 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     3241 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     3301 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     3361 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     3421 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     3481 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     3541 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     3601 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     3661 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca
     3721 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     3781 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     3841 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     3901 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     3961 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg
     4021 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     4081 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     4141 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     4201 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     4261 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     4321 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     4381 tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca ctctcagtac
     4441 aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg tgttggaggt
     4501 cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga ccgacaattg
     4561 catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac gggccagata
     4621 tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt
     4681 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
     4741 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
     4801 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
     4861 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
     4921 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
     4981 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
     5041 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
     5101 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
     5161 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg cgttttgcct
     5221 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
     5281 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
     5341 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
     5401 ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg
     5461 acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag
     5521 tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag
     5581 tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa
     5641 gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt
     5701 taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc
     5761 atcccttcag acaggatcag aagaacttag atcattatat aatacagtag caaccctcta
     5821 ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga
     5881 agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag
     5941 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     6001 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     6061 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     6121 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     6181 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     6241 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     6301 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     6361 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     6421 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     6481 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     6541 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     6601 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     6661 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     6721 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     6781 cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa ttttaaaaga
     6841 aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac
     6901 atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac
     6961 agggacagca gagatccagt ttggttaatt aatcgataag ctttgcaaag atggataaag
     7021 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     7081 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     7141 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     7201 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     7261 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     7321 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     7381 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     7441 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     7501 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     7561 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     7621 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     7681 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     7741 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     7801 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     7861 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     7921 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     7981 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     8041 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     8101 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     8161 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     8221 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattag cttggtacta
     8281 atacgactca ctatagggag acccaagctg gctaggtaag cttgatatcg aattcctgca
     8341 gcccggggga tcccgccacc atgaaaaagc ctgaactcac cgcgacgtct gtcgagaagt
     8401 ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag ggcgaagaat
     8461 ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta aatagctgcg
     8521 ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc gcgctcccga
     8581 ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc atctcccgcc
     8641 gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct gttctgcagc
     8701 cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg agcgggttcg
     8761 gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc atatgcgcga
     8821 ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc agtgcgtccg
     8881 tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa gtccggcacc
     8941 tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc ataacagcgg
     9001 tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc aacatcttct
     9061 tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag cggaggcatc
     9121 cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt cttgaccaac
     9181 tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag ggtcgatgcg
     9241 acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc cgcagaagcg
     9301 cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac cgacgcccca
     9361 gcactcgtcc gagggcaaag gaaggatccg gcgcaacaaa cttctctctg ctgaaacaag
     9421 ccggagatgt cgaagagaat cctggaccgc tgcgtatccc cgtagatgcc agcacgagcc
     9481 gccgcttcac gccgccttcc accgcgctga gcccaggcaa gatgagcgag gcgttgccgc
     9541 tgggcgcccc ggacgccggc gctgccctgg ccggcaagct gaggagcggc gaccgcagca
     9601 tggtggaggt gctggccgac cacccgggcg agctggtgcg caccgacagg cccaacttcc
     9661 tctgctccgt gctgcctacg cactggcgct gcaacaagac cctgcccatc gctttcaagg
     9721 tggtggccct aggggatgtt ccagatggca ctctggtcac tgtgatggct ggcaatgatg
     9781 aaaactactc ggctgagctg agaaatgcta ccgcagccat gaagaaccag gttgcaagat
     9841 ttaatgacct caggtttgtc ggtcgaagtg gaagagggaa aagcttcact ctgaccatca
     9901 ctgtcttcac aaacccaccg caagtcgcca cctaccacag agccatcaaa atcacagtgg
     9961 atgggccccg agaacctcga agacatcggc agaaactaga tgatcagacc aagcccggga
    10021 gcttgtcctt ttccgagcgg ctcagtgaac tggagcagct gcggcgcaca gccatgaggg
    10081 tcagcccaca ccacccagcc cccacgccca accctcgtgc ctccctgaac cactccactg
    10141 cctttaaccc tcagcctcag agtcagatgc aggatacaag gcagatccaa ccatccccac
    10201 cgtggtccta cgatcagtcc taccaatacc tgggatccat tgcctctcct tctgtgcacc
    10261 cagcaacgcc catttcacct ggacgtgcca gcggcatgac aaccctctct gcagaacttt
    10321 ccagtcgact ctcaacggca cccgacctga cagcgttcag cgacccgcgc cagttccccg
    10381 cgctgccctc catctccgac ccccgcatgc actatccagg cgccttcacc tactccccga
    10441 cgccggtcac ctcgggcatc ggcatcggca tgtcggccat gggctcggcc acgcgctacc
    10501 acacctacct gccgccgccc taccccggct cgtcgcaagc gcagggaggc ccgttccaag
    10561 ccagctcgcc ctcctaccac ctgtactacg gcgcctcggc cggctcctac cagttctcca
    10621 tggtgggcgg cgagcgctcg ccgccgcgca tcctgccgcc ctgcaccaac gcctccaccg
    10681 gctccgcact gctcaacccc agcctcccga accagagcga cgtggtggag gccgagggca
    10741 gccacagcaa ctcccccacc aacatggcgc cctccgcgcg cctggaggag gccgtgtgga
    10801 ggccctactg actagctaag ttagaactat ttcctggctg ttacgcggga caaatctttc
    10861 tact
//
