LOCUS       sequence-336436-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 V63E mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_V63E_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     138..159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(278..298)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        283..612
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      463..598
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        660..707
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             726..1100
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1230..1351
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1267..1286)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1321..1340
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1400..1416)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1400..1416)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1413..1435)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1424..1440
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1448..1454,1455..1472,1473..1478))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1493..1514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1631..1648)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1802..2390)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1882..1901)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2561..3352,3353..3421))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3184..3203
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3422..3526)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3601..3620)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3792..4171
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4173..4371
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4326..4346
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4389..4569
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4616..4741
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5234..5467
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5652..5696
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5994..6111
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6247..7425
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6478..7416
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7373..7393
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7442..7461
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7442..7460
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7524..8546
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7524..8546
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8556..8612
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(10166..10199)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10224..10404
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10430..10447)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10436..10660
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10706..10865,1..269)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10793..10812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
       61 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
      121 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
      181 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
      241 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
      301 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca
      361 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
      421 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
      481 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
      541 gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
      601 ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga tcagcacgtg
      661 ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag gtgaggaact
      721 aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag
      781 cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg
      841 ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc
      901 cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt
      961 cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg
     1021 agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg
     1081 tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg
     1141 aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg
     1201 atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca
     1261 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     1321 gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct
     1381 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     1441 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     1501 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     1561 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     1621 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     1681 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1741 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     1801 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     1861 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     1921 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     1981 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     2041 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     2101 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     2161 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     2221 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     2281 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     2341 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     2401 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     2461 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     2521 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     2581 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2641 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2701 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2761 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2821 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2881 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2941 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     3001 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     3061 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     3121 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     3181 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3241 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3301 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3361 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3421 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3481 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3541 aagtgccacc tgacgtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     3601 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     3661 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     3721 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     3781 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
     3841 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
     3901 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
     3961 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
     4021 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
     4081 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
     4141 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
     4201 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
     4261 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
     4321 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
     4381 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4441 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4501 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4561 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     4621 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     4681 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     4741 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     4801 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     4861 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     4921 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     4981 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     5041 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     5101 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     5161 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     5221 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     5281 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     5341 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     5401 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     5461 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     5521 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     5581 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     5641 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     5701 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     5761 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     5821 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     5881 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     5941 gatcggcact gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa
     6001 agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca
     6061 gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat
     6121 tacagggaca gcagagatcc agtttggtta attaatcgat aagctttgca aagatggata
     6181 aagttttaaa cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg
     6241 ggaattggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag
     6301 ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg
     6361 gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata
     6421 agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta
     6481 agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct
     6541 tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt
     6601 gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag
     6661 gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc
     6721 gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt
     6781 ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt
     6841 ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc
     6901 ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg
     6961 gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg
     7021 gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa
     7081 tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc
     7141 tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac
     7201 ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat
     7261 gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg
     7321 atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct
     7381 cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat tagcttggta
     7441 ctaatacgac tcactatagg gagacccaag ctggctaggt aagcttgata tcgaattcct
     7501 gcagcccggg ggatcccgcc accatgaaaa agcctgaact caccgcgacg tctgtcgaga
     7561 agtttctgat cgaaaagttc gacagcgtct ccgacctgat gcagctctcg gagggcgaag
     7621 aatctcgtgc tttcagcttc gatgtaggag ggcgtggata tgtcctgcgg gtaaatagct
     7681 gcgccgatgg tttctacaaa gatcgttatg tttatcggca ctttgcatcg gccgcgctcc
     7741 cgattccgga agtgcttgac attggggaat tcagcgagag cctgacctat tgcatctccc
     7801 gccgtgcaca gggtgtcacg ttgcaagacc tgcctgaaac cgaactgccc gctgttctgc
     7861 agccggtcgc ggaggccatg gatgcgatcg ctgcggccga tcttagccag acgagcgggt
     7921 tcggcccatt cggaccgcaa ggaatcggtc aatacactac atggcgtgat ttcatatgcg
     7981 cgattgctga tccccatgtg tatcactggc aaactgtgat ggacgacacc gtcagtgcgt
     8041 ccgtcgcgca ggctctcgat gagctgatgc tttgggccga ggactgcccc gaagtccggc
     8101 acctcgtgca cgcggatttc ggctccaaca atgtcctgac ggacaatggc cgcataacag
     8161 cggtcattga ctggagcgag gcgatgttcg gggattccca atacgaggtc gccaacatct
     8221 tcttctggag gccgtggttg gcttgtatgg agcagcagac gcgctacttc gagcggaggc
     8281 atccggagct tgcaggatcg ccgcggctcc gggcgtatat gctccgcatt ggtcttgacc
     8341 aactctatca gagcttggtt gacggcaatt tcgatgatgc agcttgggcg cagggtcgat
     8401 gcgacgcaat cgtccgatcc ggagccggga ctgtcgggcg tacacaaatc gcccgcagaa
     8461 gcgcggccgt ctggaccgat ggctgtgtag aagtactcgc cgatagtgga aaccgacgcc
     8521 ccagcactcg tccgagggca aaggaaggat ccggcgcaac aaacttctct ctgctgaaac
     8581 aagccggaga tgtcgaagag aatcctggac cgatgcgtat ccccgtagat gccagcacga
     8641 gccgccgctt cacgccgcct tccaccgcgc tgagcccagg caagatgagc gaggcgttgc
     8701 cgctgggcgc cccggacgcc ggcgctgccc tggccggcaa gctgaggagc ggcgaccgca
     8761 gcatggtgga ggtgctggcc gaccacccgg gcgagctgga gcgcaccgac agccccaact
     8821 tcctctgctc cgtgctgcct acgcactggc gctgcaacaa gaccctgccc atcgctttca
     8881 aggtggtggc cctaggggat gttccagatg gcactctggt cactgtgatg gctggcaatg
     8941 atgaaaacta ctcggctgag ctgagaaatg ctaccgcagc catgaagaac caggttgcaa
     9001 gatttaatga cctcaggttt gtcggtcgaa gtggaagagg gaaaagcttc actctgacca
     9061 tcactgtctt cacaaaccca ccgcaagtcg ccacctacca cagagccatc aaaatcacag
     9121 tggatgggcc ccgagaacct cgaagacatc ggcagaaact agatgatcag accaagcccg
     9181 ggagcttgtc cttttccgag cggctcagtg aactggagca gctgcggcgc acagccatga
     9241 gggtcagccc acaccaccca gcccccacgc ccaaccctcg tgcctccctg aaccactcca
     9301 ctgcctttaa ccctcagcct cagagtcaga tgcaggatac aaggcagatc caaccatccc
     9361 caccgtggtc ctacgatcag tcctaccaat acctgggatc cattgcctct ccttctgtgc
     9421 acccagcaac gcccatttca cctggacgtg ccagcggcat gacaaccctc tctgcagaac
     9481 tttccagtcg actctcaacg gcacccgacc tgacagcgtt cagcgacccg cgccagttcc
     9541 ccgcgctgcc ctccatctcc gacccccgca tgcactatcc aggcgccttc acctactccc
     9601 cgacgccggt cacctcgggc atcggcatcg gcatgtcggc catgggctcg gccacgcgct
     9661 accacaccta cctgccgccg ccctaccccg gctcgtcgca agcgcaggga ggcccgttcc
     9721 aagccagctc gccctcctac cacctgtact acggcgcctc ggccggctcc taccagttct
     9781 ccatggtggg cggcgagcgc tcgccgccgc gcatcctgcc gccctgcacc aacgcctcca
     9841 ccggctccgc gctgctcaac cccagcctcc cgaaccagag cgacgtggtg gaggccgagg
     9901 gcagccacag caactccccc accaacatgg cgccctccgc gcgcctggag gaggccgtgt
     9961 ggaggcccta ctgactagct aagttagaac tatttcctgg ctgttacgcg tggcgatcgt
    10021 ggctactgac gggcttgtta aacagtgaac cgcccgcccc acgacccgca gcgcccgacc
    10081 gaaaggagcg cacgacccca tgcatcgaac aaaagaaaag gggggactgg aagggctaat
    10141 tcactcccaa cgaagacaag atatcataac ttcgtatagc atacattata cgaagttatc
    10201 gtctagctgg tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
    10261 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
    10321 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
    10381 agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg
    10441 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
    10501 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
    10561 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
    10621 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
    10681 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
    10741 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
    10801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
    10861 gggct
//
