LOCUS       sequence-336437-       10353 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG
            at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 
            170125.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Katrekar D, Xiang Y, Palmer N, Saha A, Meluzzi D, Mali P
  TITLE     Comprehensive interrogation of the ADAR2 deaminase domain for 
            engineering enhanced RNA editing activity and specificity.
  JOURNAL   Elife. 2022 Jan 19;11. pii: 75555. doi: 10.7554/eLife.75555.
  PUBMED    35044296
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2022-01-19"; volume: "11. pii: 75555. doi"; pages: " 
            10.7554/eLife.75555"
COMMENT     Sequence Label: MS2-adRNA (5'UAG)
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     133..150
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     LTR             176..409
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    481..1069
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             587..598
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     996..1016
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1085..2110)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(2111..2167)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(2177..2884)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2452..2471)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2721..2739
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      2881..2890
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(2909..3120)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     misc_RNA        complement(3184..3202)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(3225..3243)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(3248..3561)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     complement(3309..3329)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     misc_RNA        complement(3596..3614)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        complement(3637..3655)
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     promoter        complement(3658..3906)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(3716..3735)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3886..3906)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     misc_feature    3957..4074
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(4372..4416)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4601..4834
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    5327..5452
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             5499..5679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(5697..5895)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5722..5742)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5897..6276
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     6448..6467
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        6542..6646
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6647..6715,6716..7507)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6865..6884)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7678..8266
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8167..8186
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8420..8437
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8554..8575
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8590..8595,8596..8613,8614..8620)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8628..8644
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8633..8655
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8652..8668
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8652..8668
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    8717..8838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8728..8747)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8782..8801
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(8968..9342)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(9361..9408)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(9456..9785)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9470..9605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9524..9543)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     9770..9790
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(9799..10227)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9909..9930)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10121..10140
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    join(10273..10353,1..144)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
       61 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
      121 atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctgcta
      181 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
      241 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
      301 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
      361 caagcaaaaa gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt
      421 tcttttaaaa agtggctaag atctacagct gccttgtaag tcattggtct taaagtcgac
      481 gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga agacgcggaa
      541 gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg agggccgaag
      601 ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg agcagccaag
      661 gaaaggacga tgatttcccc gacaacacca cggaattgtc agtgcccaac agccgagccc
      721 ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata gggaggggga
      781 aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca gtgggggttg
      841 cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac aactcctcat
      901 aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata cgggaagcaa
      961 tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga gcaacatagt
     1021 taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg tcgacttaac
     1081 gcgtttattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
     1141 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
     1201 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
     1261 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
     1321 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
     1381 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
     1441 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
     1501 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
     1561 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
     1621 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
     1681 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
     1741 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
     1801 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
     1861 tgaactcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
     1921 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
     1981 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
     2041 cggacacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
     2101 gctttttcat cggtccagga ttctcttcga catctccggc ttgtttcagc agagagaagt
     2161 ttgttgcgcc ggatcccttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
     2221 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
     2281 tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag gtggtcttga
     2341 cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc tcgcccttca
     2401 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg gtcttcttct
     2461 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
     2521 cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc tcgaagttca
     2581 tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg gggatgtcgg
     2641 cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg atgtcccagg
     2701 cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg
     2761 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgt
     2821 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca
     2881 ccatggtggc agcgctctag aaccggtcct gtgttctggc ggcaagcccg ttgcgaaaaa
     2941 gaacgttcac ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc
     3001 ggagccagta cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg
     3061 atcaattgcc gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc
     3121 actgacgggc accggagcca attcccacgc ctttcaagac ctagctagcg aattcaaaaa
     3181 aagacatggg tgatcctcat gttgcagact cgtgctgggc aaagacatgg gtgatcctca
     3241 tgttccaaac aaggcttttc tccaagggat atttatagtc tcaaaacaca caattacttt
     3301 acagttaggg tgagtttcct tttgtgctgt tttttaaaat aataatttag tatttgtatc
     3361 tcttatagaa atccaagcct atcatgtaaa atgtagctag tattaaaaag aacagattat
     3421 ctgtctttta tcgcacatta agcctctata gttactagga aatattatat gcaaattaac
     3481 cggggcaggg gagtagccga gcttctccca caagtctgtg cgagggggcc ggcgcgggcc
     3541 tagagatggc ggcgtcggat ctatctgaga ctgggcgact cagtacgaaa aaaagacatg
     3601 ggtgatcctc atgttgcaga ctcgtgctgg gcaaagacat gggtgatcct catgttcggt
     3661 gtttcgtcct ttccacaaga tatataaagc caagaaatcg aaatactttc aagttacggt
     3721 aagcatatga tagtccattt taaaacataa ttttaaaact gcaaactacc caagaaatta
     3781 ttactttcta cgtcacgtat tttgtactaa tatctttgtg tttacagtca aattaattct
     3841 aattatctct ctaacagcct tgtatcgtat atgcaaatat gaaggaatca tgggaaatag
     3901 gccctcggta ccttaattaa ccaaactgga tctctgctgt ccctgtaata aacccgaaaa
     3961 ttttgaattt ttgtaatttg tttttgtaat tctttagttt gtatgtctgt tgctattatg
     4021 tctactattc tttcccctgc actgtacccc ccaatccccc cttttctttt aaaattgtgg
     4081 atgaatactg ccatttgtct gcagaattgg cgcacgcagt gccgatccgt tcactaatcg
     4141 aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg
     4201 tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct
     4261 attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat
     4321 tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc
     4381 ccatttatct aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc
     4441 aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt
     4501 cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc
     4561 agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag
     4621 gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg
     4681 caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc
     4741 agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc
     4801 tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg
     4861 caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt
     4921 atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat
     4981 cagcggccgc ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta
     5041 aagcttcctt ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg
     5101 tattatataa tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca
     5161 gtatttgtct acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta
     5221 gctccctgct tgcccatact atatgtttta atttatattt tttctttccc cctggcctta
     5281 accgaatttt ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc
     5341 catctctctc cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc
     5401 cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg
     5461 gtttcccttt cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga
     5521 ctaaaagggt ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac
     5581 ttgaagcact caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga
     5641 gagctcccag gctcagatct ggtctaacca gagagaccca gtacaggcaa aacgcgctgc
     5701 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     5761 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     5821 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     5881 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5941 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     6001 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     6061 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     6121 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     6181 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     6241 ttgattacta ttaataacta gtcaataatc aatgtcaacg cgtatatctg gcccgtacat
     6301 cgcgaagcag cgcaaaacgc ctaaccctaa gcagattctt catgcaattg tcggtcaagc
     6361 cttgccttgt tgtagcttaa attttgctcg cgcactactc agcgacctcc aacacacaag
     6421 cagggagcag atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     6481 ataggggatc gggagatctc ccgatccgtc gacgtcaggt ggcacttttc ggggaaatgt
     6541 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6601 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6661 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6721 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6781 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6841 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6901 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6961 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     7021 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     7081 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     7141 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     7201 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     7261 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     7321 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     7381 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     7441 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7501 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7561 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7621 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7681 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7741 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7801 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7861 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7921 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7981 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     8041 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     8101 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     8161 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     8221 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     8281 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     8341 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     8401 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     8461 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8521 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8581 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8641 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctctagc tagaggtcga
     8701 cggtatacag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     8761 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     8821 agctgcaata aacaagttgg ggtgggcgaa gaactccagc atgagatccc cgcgctggag
     8881 gatcatccag ccggcgtccc ggaaaacgat tccgaagccc aacctttcat agaaggcggc
     8941 ggtggaatcg aaatctcgta gcacgtgtca gtcctgctcc tcggccacga agtgcacgca
     9001 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
     9061 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
     9121 cagctcgtcc aggccgcgca cccacaccca ggccagggtg ttgtccggca ccacctggtc
     9181 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
     9241 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
     9301 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt
     9361 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac gtgctgatca
     9421 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     9481 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     9541 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     9601 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     9661 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     9721 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     9781 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     9841 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     9901 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     9961 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    10021 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    10081 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    10141 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    10201 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
    10261 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
    10321 cctccccctt gctgtcctgc cccaccccac ccc
//
