LOCUS       sequence-336438-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 D66V mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_D66V_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(80..100)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        85..414
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      265..400
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        462..509
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             528..902
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1032..1153
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1069..1088)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1123..1142
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1202..1218)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1202..1218)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1215..1237)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1226..1242
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1250..1256,1257..1274,1275..1280))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1295..1316
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1433..1450)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1604..2192)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1684..1703)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2363..3154,3155..3223))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2986..3005
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3224..3328)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3403..3422)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3594..3973
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3975..4173
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4128..4148
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4191..4371
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4418..4543
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5036..5269
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5454..5498
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5796..5913
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6049..7227
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6280..7218
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7175..7195
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7244..7263
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7244..7262
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7326..8348
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7326..8348
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8358..8414
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9968..10001)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10026..10206
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10232..10249)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10238..10462
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10508..10865,1..71)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10595..10614)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10805..10826
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
       61 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
      121 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
      181 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
      241 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
      301 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
      361 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
      421 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
      481 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
      541 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
      601 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
      661 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
      721 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
      781 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
      841 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
      901 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
      961 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     1021 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     1081 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     1141 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     1201 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     1261 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     1321 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     1381 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     1441 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     1501 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     1561 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     1621 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     1681 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     1741 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     1801 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     1861 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     1921 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     1981 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     2041 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     2101 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     2161 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     2221 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     2281 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     2341 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     2401 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     2461 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     2521 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     2581 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     2641 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     2701 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     2761 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     2821 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     2881 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     2941 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     3001 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     3061 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     3121 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     3181 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     3241 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     3301 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
     3361 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
     3421 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
     3481 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
     3541 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
     3601 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     3661 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     3721 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     3781 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     3841 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     3901 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     3961 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
     4021 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
     4081 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
     4141 taggcgtgta cggtgggagg tctatataag cagcgcgttt tgcctgtact gggtctctct
     4201 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4261 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4321 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc
     4381 gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct
     4441 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
     4501 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
     4561 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
     4621 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
     4681 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
     4741 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
     4801 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
     4861 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
     4921 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     4981 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     5041 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc
     5101 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     5161 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     5221 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     5281 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     5341 aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca
     5401 attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg
     5461 aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa
     5521 attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa
     5581 tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt
     5641 ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag
     5701 gtggagagag agacagagac agatccattc gattagtgaa cggatcggca ctgcgtgcgc
     5761 caattctgca gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     5821 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     5881 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
     5941 ccagtttggt taattaatcg ataagctttg caaagatgga taaagtttta aacagagagg
     6001 aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc
     6061 cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc
     6121 aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac
     6181 tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg
     6241 aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc
     6301 cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg
     6361 ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg
     6421 ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg
     6481 ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct
     6541 ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt
     6601 gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac
     6661 ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg
     6721 agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg
     6781 ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg
     6841 gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg
     6901 ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc
     6961 gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc
     7021 ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac
     7081 actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa
     7141 tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt
     7201 ttttttcttc catttcaggt gtcgtgagga attagcttgg tactaatacg actcactata
     7261 gggagaccca agctggctag gtaagcttga tatcgaattc ctgcagcccg ggggatcccg
     7321 ccaccatgaa aaagcctgaa ctcaccgcga cgtctgtcga gaagtttctg atcgaaaagt
     7381 tcgacagcgt ctccgacctg atgcagctct cggagggcga agaatctcgt gctttcagct
     7441 tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag ctgcgccgat ggtttctaca
     7501 aagatcgtta tgtttatcgg cactttgcat cggccgcgct cccgattccg gaagtgcttg
     7561 acattgggga attcagcgag agcctgacct attgcatctc ccgccgtgca cagggtgtca
     7621 cgttgcaaga cctgcctgaa accgaactgc ccgctgttct gcagccggtc gcggaggcca
     7681 tggatgcgat cgctgcggcc gatcttagcc agacgagcgg gttcggccca ttcggaccgc
     7741 aaggaatcgg tcaatacact acatggcgtg atttcatatg cgcgattgct gatccccatg
     7801 tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc gtccgtcgcg caggctctcg
     7861 atgagctgat gctttgggcc gaggactgcc ccgaagtccg gcacctcgtg cacgcggatt
     7921 tcggctccaa caatgtcctg acggacaatg gccgcataac agcggtcatt gactggagcg
     7981 aggcgatgtt cggggattcc caatacgagg tcgccaacat cttcttctgg aggccgtggt
     8041 tggcttgtat ggagcagcag acgcgctact tcgagcggag gcatccggag cttgcaggat
     8101 cgccgcggct ccgggcgtat atgctccgca ttggtcttga ccaactctat cagagcttgg
     8161 ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg atgcgacgca atcgtccgat
     8221 ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag aagcgcggcc gtctggaccg
     8281 atggctgtgt agaagtactc gccgatagtg gaaaccgacg ccccagcact cgtccgaggg
     8341 caaaggaagg atccggcgca acaaacttct ctctgctgaa acaagccgga gatgtcgaag
     8401 agaatcctgg accgatgcgt atccccgtag atgccagcac gagccgccgc ttcacgccgc
     8461 cttccaccgc gctgagccca ggcaagatga gcgaggcgtt gccgctgggc gccccggacg
     8521 ccggcgctgc cctggccggc aagctgagga gcggcgaccg cagcatggtg gaggtgctgg
     8581 ccgaccaccc gggcgagctg gtgcgcaccg tcagccccaa cttcctctgc tccgtgctgc
     8641 ctacgcactg gcgctgcaac aagaccctgc ccatcgcttt caaggtggtg gccctagggg
     8701 atgttccaga tggcactctg gtcactgtga tggctggcaa tgatgaaaac tactcggctg
     8761 agctgagaaa tgctaccgca gccatgaaga accaggttgc aagatttaat gacctcaggt
     8821 ttgtcggtcg aagtggaaga gggaaaagct tcactctgac catcactgtc ttcacaaacc
     8881 caccgcaagt cgccacctac cacagagcca tcaaaatcac agtggatggg ccccgagaac
     8941 ctcgaagaca tcggcagaaa ctagatgatc agaccaagcc cgggagcttg tccttttccg
     9001 agcggctcag tgaactggag cagctgcggc gcacagccat gagggtcagc ccacaccacc
     9061 cagcccccac gcccaaccct cgtgcctccc tgaaccactc cactgccttt aaccctcagc
     9121 ctcagagtca gatgcaggat acaaggcaga tccaaccatc cccaccgtgg tcctacgatc
     9181 agtcctacca atacctggga tccattgcct ctccttctgt gcacccagca acgcccattt
     9241 cacctggacg tgccagcggc atgacaaccc tctctgcaga actttccagt cgactctcaa
     9301 cggcacccga cctgacagcg ttcagcgacc cgcgccagtt ccccgcgctg ccctccatct
     9361 ccgacccccg catgcactat ccaggcgcct tcacctactc cccgacgccg gtcacctcgg
     9421 gcatcggcat cggcatgtcg gccatgggct cggccacgcg ctaccacacc tacctgccgc
     9481 cgccctaccc cggctcgtcg caagcgcagg gaggcccgtt ccaagccagc tcgccctcct
     9541 accacctgta ctacggcgcc tcggccggct cctaccagtt ctccatggtg ggcggcgagc
     9601 gctcgccgcc gcgcatcctg ccgccctgca ccaacgcctc caccggctcc gcgctgctca
     9661 accccagcct cccgaaccag agcgacgtgg tggaggccga gggcagccac agcaactccc
     9721 ccaccaacat ggcgccctcc gcgcgcctgg aggaggccgt gtggaggccc tactgactag
     9781 ctaagttaga actatttcct ggctgttacg cgccggacca tggactactg acgggcttgt
     9841 taaacagtga accgcccgcc ccacgacccg cagcgcccga ccgaaaggag cgcacgaccc
     9901 catgcatcga acaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     9961 agatatcata acttcgtata gcatacatta tacgaagtta tcgtctagct ggtccggact
    10021 gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc taactaggga
    10081 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
    10141 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
    10201 ctagcagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca
    10261 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
    10321 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
    10381 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
    10441 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg
    10501 tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
    10561 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
    10621 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
    10681 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
    10741 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
    10801 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
    10861 gattt
//
