LOCUS       sequence-33645-e         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Cross FR, Pecani K
  TITLE     Efficient and rapid exact gene replacement without selection.
  JOURNAL   Yeast. 2011 Feb . 28(2):167-79.
  PUBMED    21246629
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2011
            Feb . 28(2):167-79."
COMMENT     Sequence Label: FC512
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(47..63)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(47..63)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc cactagttct
       61 agatctcgag atagttttta tacaatacat atatatatat atatatatat atttacaaga
      121 aaagcatggc ttatgtatta tacttgctta tgtaccaata tatacatata tggccttatt
      181 agggcaacaa acgccaaaaa taaaaacgaa cctaaatgag aaaaaaagag ttaacaaaaa
      241 tttggaaaac aacaagaact caaaaaaaaa aattggtgga aggaccaagt cctcttgaaa
      301 gaaaatttaa tactgctttt ccagaatatc atattggaaa aaatattcaa gccagcacat
      361 cagctacagt ggaaaatagc ccagatcaaa tagaactatc cttcgcatat gagcggtgaa
      421 ttagcaaatt acaaaagact tgagaaagtc ggtgaaggta catacggtgt tgtttataaa
      481 gcgttagact taagacctgg ccaaggtcaa agagtagtcg cattgaagaa aataagacta
      541 gagagtgaag acgagggtgt tcccagtaca gccatcagag aaatctcatt attgaaggaa
      601 ttaaaagacg ataatattgt cagattatac gatattgttc actctgatgc acacaagcta
      661 tatcttgttt ttgagttcct cgatttggac ctgaaaagat atatggaggg tattccaaag
      721 gaccaaccgt taggagctga tattgttaag aagtttatga tgcaactttg taagggtatt
      781 gcatactgcc actcacaccg tattctgcat cgtgatttaa aaccgcagaa cttattgatt
      841 aacaaagatg ggaatctaaa actaggtgat tttggcttag cgggcgcctt tggtgttccg
      901 ttgagagctt acacacatga aattgttact ctatggtata gagctcc
//
