LOCUS       sequence-336456-        9140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Alpha1 Basic cloning vector GB2.0 compatible P[acman]-derived 
            backbone. Allows for copy induction from low to high using AutoFOS 
            or similar. Requires EPI300 bacterial strain (DH10B Derived) or 
            similar. uses blue-white screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9140)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 9140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: P[acman]-Alpha1
FEATURES             Location/Qualifiers
     source          1..9140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(348..1319)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1319..2494)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    2820..3070
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3073..3828)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      3919..4138
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4225..4860
                     /label=oriV
                     /note="incP origin of replication"
     primer_bind     complement(6086..6105)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             6097..6912
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             6097..6912
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(6170..6189)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     protein_bind    7043..7112
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(7327..7357,7358..7559)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        7590..7637
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7691..7709
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7691..7708
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    7716..7772
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7774..7793)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7775..7793)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7800..7817)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7800..7816)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7809..7831)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(8025..8044)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(8081..8635,8636..8666)
                     /label=P element 5' end
     misc_feature    join(8942..9140,1..275)
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 gactaataat cagaccgacg atacgagtgg gaccatggtc ccagactaat aatcagaccg
       61 acgatacgag tgggaccgtg gtcccagtct gattatcaga ccgacgatac gagtgggacc
      121 gtggtcccag actaataatc agaccgacga tacgagtggg accgtggtcc cagactaata
      181 atcagaccga cgatacgagt gggaccgtgg tcccagtctg attatcagac cgacgataca
      241 agtggaacag tgggcccaga gagaatattc aggccagtta tgctttctgg cctgtaacaa
      301 aggacattaa gtaaagacag ataaacgtag actaaaacgt ggtcgcatca gggtgctggc
      361 ttttcaagtt ccttaagaat ggcctcaatt ttctctatac actcagttgg aacacgcgac
      421 ctgtccaggt taagcaccat tttatcgccc ttatacaata ctgtcgctcc aggagcaaac
      481 tgatgtcgtg agcttaaact agttcttgat gcagatgacg ttttaagcac agaagttaaa
      541 agagtgataa cttcttcagc ttcaaatatc accccagctt ttttctgctc atgaaggtta
      601 gatgcctgct gcttaagtaa ttcctcttta tctgtaaagg ctttttgaag tgcatcacct
      661 gaccgggcag atagttcacc ggggtgagaa aaaagagcaa caactgattt aggcaatttg
      721 gcggtgttga tacagcgggt aataatctta cgtgaaatat tttccgcatc agccagcgca
      781 gaaatatttc cagcaaattc attctgcaat cggcttgcat aacgctgacc acgttcataa
      841 gcacttgttg ggcgataatc gttacccaat ctggataatg cagccatctg ctcatcatcc
      901 agctcgccaa ccagaacacg ataatcactt tcggtaagtg cagcagcttt acgacggcga
      961 ctcccatcgg caatttctat gacaccagat actcttcgac cgaacgccgg tgtctgttga
     1021 ccagtcagta gaaaagaagg gatgagatca tccagtgcgt cctcagtaag cagctcctgg
     1081 tcacgttcat tacctgacca tacccgagag gtcttttcaa cactatcacc ccggagcact
     1141 tcaagagtaa acttcacatc ccgaccacat acaggcaaag taatggcatt accgcgagcc
     1201 attactccta cgcgcgcaat taacgaatcc accatcgggg cagctggtgt cgataacgaa
     1261 gtatcttcaa ccggttgagt attgagcgta tgttttggaa taacaggcgc acgcttcatt
     1321 atctaatctc ccagcgtggt ttaatcagac gatcgaaaat ttcattgcag acaggttccc
     1381 aaatagaaag agcatttctc caggcaccag ttgaagagcg ttgatcaatg gcctgttcaa
     1441 aaacagttct catccggatc tgacctttac caacttcatc cgtttcacgt acaacatttt
     1501 ttagaaccat gcttccccag gcatcccgaa tttgctcctc catccacggg gactgagagc
     1561 cattactatt gctgtatttg gtaagcaaaa tacgtacatc aggctcgaac cctttaagat
     1621 caacgttctt gagcagatca cgaagcatat cgaaaaactg cagtgcggag gtgtagtcaa
     1681 acaactcagc aggcgtggga acaatcagca catcagcagc acatacgaca ttaatcgtgc
     1741 cgatacccag gttaggcgcg ctgtcaataa ctatgacatc atagtcatga gcaacagttt
     1801 caatggccag tcggagcatc aggtgtggat cggtgggcag tttaccttca tcaaatttgc
     1861 ccattaactc agtttcaata cggtgcagag ccagacagga aggaataatg tcaagccccg
     1921 gccagcaagt gggctttatt gcataagtga catcgtcctt ttccccaaga tagaaaggca
     1981 ggagagtatc ttctgcatga atatgaagat ctggtaccca tccgtgatac attgaggctg
     2041 ttccctgggg gtcgttacct tccacgagca aaacacgtag ccccttcaga gccagatcct
     2101 gagcaagatg aacagaaact gaggttttgt aaacgccacc tttatgggca gcaaccccga
     2161 tcaccggtgg aaatacatct tcagcacgtc gcaatcgcgt accaaacaca tcacgcatat
     2221 gattaatttg ttcaattgta taaccaacac gttgctcaac ccgtcctcga atttccatat
     2281 ccgggtgcgg tagtcgccct gctttctcgg catctctgat agcctgagaa gaaaccccaa
     2341 ctaaatccgc tgcttcacct attctccagc gccgggttat tttcctcgct tccgggctgt
     2401 catcattaaa ctgtgcaatg gcgatagcct tcgtcatttc atgaccagcg tttatgcact
     2461 ggttaagtgt ttccatgagt ttcattctga acatccttta atcattgctt tgcgtttttt
     2521 tattaaatct tgcaatttac tgcaaagcaa caacaaaatc gcaaagtcat caaaaaaccg
     2581 caaagttgtt taaaataaga gcaacactac aaaaggagat aagaagagca catacctcag
     2641 tcacttatta tcactagcgc tcgccgcagc cgtgtaaccg agcatagcga gcgaactggc
     2701 gaggaagcaa agaagaactg ttctgtcaga tagctcttac gctcagcgca agaagaaata
     2761 tccaccgtgg gaaaaactcc aggtagaggt acacacgcgg atagccaatt cagagtaata
     2821 aactgtgata atcaaccctc atcaatgatg acgaactaac ccccgatatc aggtcacatg
     2881 acgaagggaa agagaaggaa atcaactgtg acaaactgcc ctcaaatttg gcttccttaa
     2941 aaattacagt tcaaaaagta tgagaaaatc catgcaggct gaaggaaaca gcaaaactgt
     3001 gacaaattac cctcagtagg tcagaacaaa tgtgacgaac caccctcaaa tctgtgacag
     3061 ataaccctca gactatcctg tcgtcatgga agtgatatcg cggaaggaaa atacgatatg
     3121 agtcgtctgg cggcctttct ttttctcaat gtatgagagg cgcattggag ttctgctgtt
     3181 gatctcatta acacagacct gcaggaagcg gcggcggaag tcaggcatac gctggtaact
     3241 ttgaggcagc tggtaacgct ctatgatcca gtcgattttc agagacacga tgcctgagcc
     3301 atccggctta cgatactgac acagggattc gtataaacgc atggcatacg gattggtgat
     3361 ttcttttgtt tcactaagcc gaaactgcgt aaaccggttc tgtaacccga taaagaaggg
     3421 aatgagatat gggttgatat gtacactgta aagccctctg gatggactgt gcgcacgttt
     3481 gataaaccaa ggaaaagatt catagccttt ttcatcgccg gcatcctctt cagggcgata
     3541 aaaaaccact tccttccccg cgaaactctt caatgcctgc cgtatatcct tactggcttc
     3601 cgcagaggtc aatccgaata tttcagcata tttagcaaca tggatctcgc agataccgtc
     3661 atgttcctgt agggtgccat cagattttct gatctggtca acgaacagat acagcatacg
     3721 tttttgatcc cgggagagac tatatgccgc ctcagtgagg tcgtttgact ggacgattcg
     3781 cgggctattt ttacgtttct tgtgattgat aaccgctgtt tccgccatga cagatccatg
     3841 tgaagtgtga caagttttta gattgtcaca ctaaataaaa aagagtcaat aagcagggat
     3901 aactttgtga aaaaacagct tcttctgagg gcaatttgtc acagggttaa gggcaatttg
     3961 tcacagacag gactgtcatt tgagggtgat ttgtcacact gaaagggcaa tttgtcacaa
     4021 caccttctct agaaccagca tggataaagg cctacaaggc gctctaaaaa agaagatcta
     4081 aaaactataa aaaaaataat tataaaaata tccccgtgga taagtggata accccaaggg
     4141 aagttttttc aggcatcgtg tgtaagcaga atatataagt gctgttccct ggtgcttcct
     4201 cgctcactcg aaattcccct ttccgacgct caccgggctg gttgccctcg ccgctgggct
     4261 ggcggccgtc tatggccctg caaacgcgcc agaaacgccg tcgaagccgt gtgcgagaca
     4321 ccgcggccgg ccgccggcgt tgtggatacc tcgcggaaaa cttggccctc actgacagat
     4381 gaggggcgga cgttgacact tgaggggccg actcacccgg cgcggcgttg acagatgagg
     4441 ggcaggctcg atttcggccg gcgacgtgga gctggccagc ctcgcaaatc tgcgaaaacg
     4501 cctgatttta cgcgagtttc ccacagatga tgtggacaag cctggggata agtgccctgc
     4561 ggtattgaca cttgaggggc gcgactactg acagatgagg ggcgcgatcc ttgacacttg
     4621 aggggcagag tgctgacaga tgaggggcgc acctattgac atttgagggg ctgtccacag
     4681 gcagaaaatc cagcatttgc aagggtttcc gcccgttttt cggccaccgc taacctgtct
     4741 tttaacctgc ttttaaacca atatttataa accttgtttt taaccagggc tgcgccctgt
     4801 gcgcgtgacc gcgcacgccg aaggggggtg cccccccttc tcgaaccctc ccggcccgct
     4861 aacgcgggcc tcccatcccc ccaggggctg cgcccctcgg ccgcgaacgg cctcacccca
     4921 aaaatggcag gggatctcga gggcttcgcc ctgtcgctcg actgcggcga gcactactgg
     4981 ctgtaaaagg acagaccaca tcatggttct gtgttcatta ggttgttctg tccattgctg
     5041 acataatccg ctccacttca acgtaacacc gcacgaagat ttctattgtt cctgaaggca
     5101 tattcaaatc gttttcgtta ccgcttgcag gcatcatgac agaacactac ttcctataaa
     5161 cgctacacag gctcctgaga ttaataatgc ggatctctac gataatggga gattttcccg
     5221 actgtttcgt tcgcttctca gtggataaca gccagcttct ctgtttaaca gacaaaaaca
     5281 gcatatccac tcagttccac atttccatat aaaggccaag gcatttattc tcaggataat
     5341 tgtttcagca tcgcaaccgc atcagactcc ggcatcgcaa actgcacccg gtgccgggca
     5401 gccacatcca gcgcaaaaac cttcgtgtag acttccgttg aactgatgga cttatgtccc
     5461 atcaggcttt gcagaacttt cagcggtata ccggcataca gcatgtgcat cgcataggaa
     5521 tggcggaacg tatgtggtgt gaccggaaca gagaacgtca caccgtcagc agcagcggcg
     5581 gcaaccgcct ccccaatcca ggtcctgacc gttctgtccg tcacttccca gatccgcgct
     5641 ttctctgtcc ttcctgtgcg acggttacgc cgctccatga gcttatcgcg aataaatacc
     5701 tgtgacggaa gatcacttcg cagaataaat aaatcctggt gtccctgttg ataccgggaa
     5761 gccctgggcc aacttttggc gaaaatgaga gtgagctgtt ggatggggat cgcctgaatc
     5821 gccccatcat ccagccagaa agtgagggag ccacggttga tgagagcttt gttgtaggtg
     5881 gaccagttgg tgattttgaa cttttgcttt gccacggaac ggtctgcgtt gtcgggaaga
     5941 tgcgtgatct gatccttcaa ctcagcaaaa gttcgattta ttcaacaaag ccacgttgtg
     6001 tctcaaaatc tctggtgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     6061 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     6121 tcttgctcaa ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg
     6181 gctcgcgata atgtcgggca atcaggtgcg acaatctacc gattgtatgg gaagcccgat
     6241 gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag
     6301 atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc
     6361 cgtactcctg atgatgcatg gttactcacc actgcgatcc cagggaaaac agcattccag
     6421 gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg
     6481 cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcga
     6541 ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac
     6601 gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc
     6661 tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag
     6721 gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat
     6781 cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt
     6841 caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat
     6901 gagtttttct aatcactaga ccaatgttac acatatatac tttagattga tttaaaactt
     6961 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gtgttgaatt
     7021 ggtcgacgat gtaggtcact gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg
     7081 gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt
     7141 ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc
     7201 tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc
     7261 ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgatcacg atgagaatgg
     7321 ccagaccatg atgaaataac ataaggtggt cccgtcggca agagacatcc acttaacgta
     7381 tgcttgcaat aagtgcgagt gaaaggaata gtattctgag tgtcgtattg agtctgagtg
     7441 agacagcgat atgattgttg attaaccctt agcatgtccg tggggtttga attaactcat
     7501 aatattaatt agacgaaatt atttttaaag ttttattttt aataatttgc gagtacgcat
     7561 aacgaattcg tctcaggaga gagacccctg ttgacaatta atcatcggca tagtatatcg
     7621 gcatagtata atacgacaag gtgaggaact aaacccagga ggcagatcat gaccatgatt
     7681 acgccaagct atttaggtga cactatagaa tactcaagct tgcatgcctg caggtcgact
     7741 ctagaggatc cccgggtacc gagctcgaat tcgccctata gtgagtcgta ttacaattca
     7801 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     7861 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     7921 ccttcccaac agttgcgcag cctgaatggc gaatgacgcc tgatgcggta ttttctcctt
     7981 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     8041 gccgcatagg gtctctcgct gtcatgagac gaattctgac gctgctgctc taaacgacgc
     8101 atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt aaacaatgaa
     8161 caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta ctattcaaac
     8221 ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg gttcaggctc
     8281 tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg tctgaccttt
     8341 tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt gggagttttc
     8401 accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac acttcggcac
     8461 gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt tcattttttt
     8521 ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca acctttcctc
     8581 tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg gctatcgacg
     8641 ggaccacctt atgttatttc atcatgggcc agacccacgt agtccagact agtggatctc
     8701 gacagcgaca cacttgcatc ggatgcagcc cggttaacgt gccggcacgg cctgggtaac
     8761 caggtatttt gtccacataa ccgtgcgcaa aatgttgtgg ataagcagga cacagcagca
     8821 atccacagca ggcatacaac cgcacaccga ggttactccg ttctacaggt tacgacgaca
     8881 tgtcaatact tgcccttgac aggcattgat ggaatcgtag tctcacgctg atagtctgat
     8941 cgacaataca agtgggaccg tggtcccaga ccgataatca gaccgacaac acgagtggga
     9001 tcgtggtccc agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagactaa
     9061 taatcagacc gacgatacga gtgggaccgt ggttccagac taataatcag accgacgata
     9121 cgagtgggac cgtggtccca
//
