LOCUS       sequence-33646-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Cross FR, Pecani K
  TITLE     Efficient and rapid exact gene replacement without selection.
  JOURNAL   Yeast. 2011 Feb . 28(2):167-79.
  PUBMED    21246629
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast. 2011
            Feb . 28(2):167-79."
COMMENT     Sequence Label: FC513
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(45..61)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(45..61)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
       61 atctcgagat agtttttata caatacatat atatatatat atatatatat ttacaagaaa
      121 agcatggctt atgtattata cttgcttatg taccaatata tacatatatg gccttattag
      181 ggcaacaaac gccaaaaata aaaacgaacc taaatgagaa aaaaagagtt aacaaaaatt
      241 tggaaaacaa caagaactca aaaaaaaaaa ttggtggaag gaccaagtcc tcttgaaaga
      301 aaatttaata ctgcttttcc agaatatcat attggaaaaa atattcaagc cagcacatca
      361 gctacagtgg aaaatagccc agatcaaata gaactatcct tcgcatatga gcggtgaatt
      421 agcaaattac aaaagacttg agaaagtcgg tgaaggtaca tacggtgttg tttataaagc
      481 gttagactta agacctggcc aaggtcaaag agtagtcgca ttgaagaaaa taagactaga
      541 gagtgaagac gagggtgttc ccagtacagc catctgatag atctcattat tgaaggaatt
      601 aaaagacgat aatattgtca gattatacga tattgttcac tctgatgcac acaagctata
      661 tcttgttttt gagttcctcg atttggacct gaaaagatat atggagggta ttccaaagga
      721 ccaaccgtta ggagctgata ttgttaagaa gtttatgatg caactttgta agggtattgc
      781 atactgccac tcacaccgta ttctgcatcg tgatttaaaa ccgcagaact tattgattaa
      841 caaagatggg aatctaaaac taggtga
//
