LOCUS       sequence-33647-e         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-G2
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      16..25
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(31..437)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    504..537
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(579..595)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(580..596)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    654..771
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cctcgccctt gctcaccatg gtggcgggat ccgggcgact cagtcaatcg gaggactggc
       61 gcgccgagtg aggggttgtg agctctttta tagagctcgg gaagcagaag cgcgcgaaca
      121 gaagcgagaa gcaggctgat tggttaattc aaataaggcg cagggtcatt tcaggtcctt
      181 gggggagcct ggaaacatct gatgggtctt aagaaactgc tgagggttgg gccatatctg
      241 gggaccatct gttcttggcc tcgggccggg gccgaaactg cggtgaccat ctgttcttgg
      301 ccccgggccg gggccgaaac tgctcaccgc agatatcctg tttggcccaa cgttagctat
      361 tttcatgtac ccgcccttga tctgaacttc tctattcttg gtttggtatt tttccatgcc
      421 ttgcaaaatg gcgttactgc agctagcttg ccaaacctac aggtggggtc tttcagcggc
      481 cgcttaagct tggaaccctt aatataactt cgtataatgt atgctatacg aagttattag
      541 gtccctcgac ctgctggaat ctcgtgaagc gagcttatcg ataccgtcga cctcgactag
      601 agcgggcccg gtactaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt
      661 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
      721 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
      781 aatactgcca tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat
      841 ggatctgtct ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc
      901 cctcggggtt gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt
      961 cactatag
//
