LOCUS       sequence-336485-        9836 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  2nd generation lentiviral transfer plasmid encoding a U6 promoter 
            expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter 
            expressing mNeonGreen.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9836)
  AUTHORS   Hamilton JR, Tsuchida CA, Nguyen DN, Shy BR, McGarrigle ER, Sandoval
            Espinoza CR, Carr D, Blaeschke F, Marson A, Doudna JA
  TITLE     Targeted delivery of CRISPR-Cas9 and transgenes enables complex 
            immune cell engineering.
  JOURNAL   Cell Rep. 2021 Jun 1;35(9):109207. doi: 
            10.1016/j.celrep.2021.109207.
  PUBMED    34077734
REFERENCE   2  (bases 1 to 9836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-1"; volume: "35(9):109207. doi"; pages: " 
            10.1016/j.celrep.2021.109207"
COMMENT     Sequence Label: pCAT003
FEATURES             Location/Qualifiers
     source          1..9836
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(115..135)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        120..449
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      300..435
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     362..381
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        497..544
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             563..937
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1067..1188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1104..1123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1158..1177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1237..1253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1237..1253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1250..1272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1261..1277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1285..1291,1292..1309,1310..1315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1330..1351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1468..1485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1639..2227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1719..1738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2398..3189,3190..3258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3021..3040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3259..3363)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3438..3457)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3623..4002
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4004..4202
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4157..4177
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4220..4400
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4447..4572
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5065..5298
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5483..5527
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5825..5942
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5993..6241
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5993..6013
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6164..6183
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        6392..7569
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6623..7560
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7517..7537
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7587..7596
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    8319..8907
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8372..8392)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8790..8801)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8979..9212
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(9238..9255)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9244..9468
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9514..9836,1..106)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9601..9620)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9811..9832
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
       61 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg
      121 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      181 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      241 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      301 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
      361 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
      421 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
      481 gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata
      541 cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc
      601 gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt
      661 cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt
      721 ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga
      781 gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc
      841 catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg
      901 caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc
      961 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     1021 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     1081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     1141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     1201 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1261 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1321 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1381 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1441 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1801 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2041 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     2581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     2641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     2701 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     2761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     2821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     2881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     2941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3001 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3301 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3361 gcgcacattt ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc
     3421 ctatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc
     3481 cctgcttgtg tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg
     3541 caaggcttga ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt
     3601 cgcgatgtac gggccagata ttgacattga ttattgacta gttattaata gtaatcaatt
     3661 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     3721 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     3781 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     3841 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     3901 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     3961 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     4021 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     4081 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     4141 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     4201 agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     4261 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     4321 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     4381 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     4441 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     4501 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     4561 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     4621 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     4681 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     4741 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     4801 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     4861 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     4921 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     4981 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     5041 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     5101 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     5161 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     5221 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     5281 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     5341 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     5401 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     5461 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     5521 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     5581 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     5641 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     5701 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     5761 attagtgaac ggatcggcac tgcgtgcgcc aattctgcag acaaatggca gtattcatcc
     5821 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     5881 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     5941 ttcgggttta ttacagggac agcagagatc cagtttggtt aattaaggta ccgagggcct
     6001 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattag
     6061 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     6121 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     6181 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     6241 cgagagtctc tcagctggta cagtttaaga gctatgctgg aaacagcata gcaagtttaa
     6301 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt tttttgaatt
     6361 cgctagctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     6421 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
     6481 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     6541 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     6601 cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt
     6661 tacgggttat ggcccttgcg tgccttgaat tacttccact ggctgcagta cgtgattctt
     6721 gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc
     6781 ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc
     6841 tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt
     6901 tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat
     6961 ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag
     7021 cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag
     7081 tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc
     7141 tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc
     7201 ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag
     7261 tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg
     7321 gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct
     7381 ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact
     7441 gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt
     7501 ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag
     7561 gtgtcgtgaa ccggttctag agcgctgcca ccatggtaag taagggcgaa gaggataata
     7621 tggcttcttt gcccgccaca cacgaactgc acatattcgg atcaataaac ggggtggatt
     7681 tcgatatggt cggccaggga acaggaaacc ctaatgacgg ctacgaagag ctcaatctca
     7741 aatctaccaa aggggactta caattctctc catggatcct agtgccacat attggatatg
     7801 gcttccatca ataccttcca tatccagacg gtatgagccc cttccaggct gcaatggtgg
     7861 acgggtctgg atatcaggtt cacagaacaa tgcaatttga agacggtgca tcccttacag
     7921 ttaattacag gtacacatac gaaggaagtc acatcaaagg ggaggcgcag gtgaagggca
     7981 ccgggttccc agctgatgga cctgtaatga caaattcact cacagccgcc gattggtgca
     8041 ggtcaaagaa gacctatcct aatgacaaaa caattatatc cacatttaaa tggtcttata
     8101 ccacaggaaa tggaaagaga tacagatcta cagctagaac aacttacaca tttgctaaac
     8161 caatggcagc caactacctt aagaatcaac caatgtatgt cttcagaaag actgaactaa
     8221 aacacagtaa aacagaactt aatttcaaag agtggcagaa ggccttcaca gacgttatgg
     8281 gtatggatga actgtacaag taaacgcgtt aagtcgacaa tcaacctctg gattacaaaa
     8341 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
     8401 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
     8461 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
     8521 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
     8581 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
     8641 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
     8701 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
     8761 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
     8821 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
     8881 ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc
     8941 tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca
     9001 acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc
     9061 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     9121 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     9181 acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct
     9241 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
     9301 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
     9361 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
     9421 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
     9481 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
     9541 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     9601 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     9661 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     9721 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     9781 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaaca
//
