LOCUS       sequence-33649-e         601 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 601)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iV2
FEATURES             Location/Qualifiers
     source          1..601
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    30..582
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    32..599
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(192..211)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     421..438
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 ctcgcccttg ctcaccatgg ttgtggccat attatcatcg tgtttttcaa aggaaaacca
       61 cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc
      121 atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca
      181 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
      241 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
      301 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
      361 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
      421 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
      481 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
      541 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
      601 a
//
