LOCUS       sequence-336498-       10850 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagBFP-tagged Src variant lacking SH2 and SH3 domains..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR Src deltaSH2/3-TagBFP
FEATURES             Location/Qualifiers
     source          1..10850
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(20..37)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(191..779)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(271..290)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(950..1741,1742..1810))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1573..1592
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1811..1915)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     1983..2001
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2039..2061)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2161..2180
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2394..2413
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        2703..2721
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2703..2720
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     LTR             2746..2979
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     3069..3085
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3070..3086
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3089..3677
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3195..3206
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     3604..3624
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3775..4473)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     promoter        complement(5664..6071)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     misc_feature    6224..6341
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(6626..6670)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6855..7088
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7585..7710
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7757..8390
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     polyA_signal    8558..8692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8582..8601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     8636..8655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(9117..9137)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     intron          9267..9332
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     promoter        complement(10466..10795)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10480..10615
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10780..10800
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
       61 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
      121 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
      181 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
      241 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
      301 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
      361 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
      421 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
      481 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
      541 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
      601 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
      661 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
      721 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
      781 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
      841 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
      901 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
      961 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     1021 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     1081 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     1141 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     1201 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     1261 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     1321 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     1381 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     1441 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     1501 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     1561 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     1621 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     1681 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     1741 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     1801 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     1861 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     1921 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     1981 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     2041 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     2101 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     2161 cggcatcaga gcagattgta ctgagagtgc accattcgac gctctccctt atgcgactcc
     2221 tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat
     2281 ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc
     2341 acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg
     2401 tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt
     2461 ccggcgtaga ggatctggct agcgatgacc ctgctgattg gttcgctgac catttccggg
     2521 tgcgggacgg cgttaccaga aactcagaag gttcgtccaa ccaaaccgac tctgacggca
     2581 gtttacgaga gagatgatag ggtctgcttc agtaagccag atgctacaca attaggcttg
     2641 tacatattgt cgttagaacg cggctacaat taatacataa ccttatgtat catacacata
     2701 cgatttaggt gacactatag aatacacgga attaattcta gatgctgcta gagattttcc
     2761 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     2821 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     2881 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caagcaaaaa
     2941 gcagatcttg tcttcgttgg gagtgaatta gcccttccag tccccccttt tcttttaaaa
     3001 agtggctaag atctacagct gccttgtaag tcattggtct taaaggtacc gagctcgaat
     3061 taattccctc gaggtcgacg gtatcgatgc ggggaggcgg cccaaaggga gatccgactc
     3121 gtctgagggc gaaggcgaag acgcggaaga ggccgcagag ccggcagcag gccgcgggaa
     3181 ggaaggtccg ctggattgag ggccgaaggg acgtagcaga aggacgtccc gcgcagaatc
     3241 caggtggcaa cacaggcgag cagccaagga aaggacgatg atttccccga caacaccacg
     3301 gaattgtcag tgcccaacag ccgagcccct gtccagcagc gggcaaggca ggcggcgatg
     3361 agttccgccg tggcaatagg gagggggaaa gcgaaagtcc cggaaaggag ctgacaggtg
     3421 gtggcaatgc cccaaccagt gggggttgcg tcagcaaaca cagtgcacac cacgccacgt
     3481 tgcctgacaa cgggccacaa ctcctcataa agagacagca accaggattt atacaaggag
     3541 gagaaaatga aagccatacg ggaagcaata gcatgataca aaggcattaa agcagcgtat
     3601 ccacatagcg taaaaggagc aacatagtta agaataccag tcaatctttc acaaattttg
     3661 taatccagag gttgattatc gataagcttg atatcaagct tgcatgcctg caggtcgact
     3721 ctagagtcgc ggcctcagtc agtcacgcat gttgcaggtg ggagttgcgg ccgcattaag
     3781 cttgtgcccc agtttgctag ggaggtcgca gtatctggcc actgccacct cgtgctgctc
     3841 gacgtaggtc tcgttgttgg cctccttgat tctttccagt ctgtagtcca catagtagac
     3901 gccaggcatc ttgaggttct tagcgggttt cttggatcta tatgtggtct tgatgtttgc
     3961 gatcagatgg ctcccgccca cgagcttcag ggccatgtcg tttctgcctt ccaggccgcc
     4021 gtcagcgggg tacagcgtct cggtgaaggc ctcccagccg agtgttttct tctgcatcac
     4081 agggccgttg gatgtgaagt tcacccctct gatcttgacg ttgtagatga ggcagccgtc
     4141 ctggaggctg gtgtcctggg tagcggtcag cacgcccccg tcttcgtatg tggtgactct
     4201 ctcccatgtg aagccctcag ggaaggactg cttgaagaag tcggggatgc cctgggtgtg
     4261 gttgatgaag gtcttgctgc cgtagaggaa gctagtagcc aggatgtcga aggcgaaggg
     4321 gagagggccg ccctcgacca ccttgattct catggtctgg gtgccctcgt agggcttgcc
     4381 ttcgccctcg gatgtgcact tgaagtgatg gttgtccacg gtgccctcca tgtacagctt
     4441 catgtgcatg ttctccttaa tcagctcgct catggtagct ccggatccta ggttctcccc
     4501 gggctggtac tgtggctcag tggacgtaaa gtagtcttcc aggaaggcct gcaggtactc
     4561 gaaggtgggc cgctcctcgg gctccttccg ccagcactgg cacataaggt catgcaggga
     4621 ctcggggcac tcggggggac aaggcatccg gtagccccgc tccacctggt ccagaacctc
     4681 acggttcacc atcccaggat agggcactct tcccttagtg gtgagctcgg tcagcagaat
     4741 cccaaaggac cacacatccg acttgatggt gaacctgccg tacagagcag cttcaggggc
     4801 ggtccacttg atggggaatt tggcaccttg ccgggctgtg tattcgttgt cttctatgag
     4861 ccgggccaac ccaaagtcgg ccactttgca caccaggttc tcccctacta ggatattggc
     4921 ggctcgaagg tcccggtgca catagttcat ccgctccaca taggccatgc ctgaagcgat
     4981 ctgagcagac atgtccacca gctggggtag ccgcaaatat ttgcccgttt cccccttgag
     5041 aaagtccagc agactcccct tgttcatgta ctctgtcaca atgtaaatgg gttcttccga
     5101 caccacagca tacagctgca ccagtttctc gtgcctcagt ttcttcatga cttgggcctc
     5161 ctgcaggaag gcctctgggg acatggtgcc tggcttcaga gtcttgatgg caaccctcgt
     5221 ggtgccgttc caggtcccca tccacacctc tccgaagcaa ccctggccca gcttgacctc
     5281 cagccgcagg gactcccggg ggatctccca cgcatccttg gccaatccct gggtctgagg
     5341 cttggatgtg ggtgccagag gccccgccct ctgcggggag gtgacggtgt ccgaggagtt
     5401 gaagcctccg aagagcttgg gctcggccgc gggcggcacg aaggcggcgc tgggcccgcg
     5461 gtggccgtcg gcggaggcgg gcttgctcgg tgtctgtgag gccgggaagg cgccccctgc
     5521 cccgtgcacg ttttccgagg gctccaggct gcggcgccgc tggctggcgt ccttgggctt
     5581 gctcttgttg ctgcccatgg atccactgtc cacctgcctt gctccacttg acgcgtgaga
     5641 attctcgaga gctccagatc ccccccgggc gactcagtct gtcggaggac tggcgcgccg
     5701 agtgaggggt tgtgagctct tttatagagc tcgggaagca gaagcgcgcg aacagaagcg
     5761 agaagcaggc tgattggtta attcaaataa ggcgcagggt catttcaggt ccttggggga
     5821 gcctggaaac atctgatggg tcttaagaaa ctgctgaggg ttgggccata tctggggacc
     5881 atctgttctt ggcctcgggc cggggccgaa actgcggtga ccatctgttc ttggccccgg
     5941 gccggggccg aaactgctca ccgcagatat cctgtttggc ccaacgttag ctattttcat
     6001 gtacccgccc ttgatctgaa cttctctatt cttggtttgg tatttttcca tgccttgcaa
     6061 aatggcgtta ctgcagctag cttgccaaac ctacaggtgg ggtctttcat tccccccttt
     6121 ttctggagac taaataaaat cttttatttt atcggggctg caggaattcg atatcaagct
     6181 tatcgatcca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
     6241 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
     6301 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
     6361 tttggcgata ccgtcgagat ccgttcacta atcgaatgga tctgtctctg tctctctctc
     6421 caccttcttc ttctattcct tcgggcctgt cgggtcccct cggggttggg aggtgggtct
     6481 gaaacgataa tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa
     6541 ctattcttaa acctaccaag cctcctacta tcattatgaa taattttata taccacagcc
     6601 aatttgttat gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt
     6661 gttcattctt ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt
     6721 aattgttaat ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag
     6781 atttattact ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc
     6841 aaccccaaat ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg
     6901 cctggagctg cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag
     6961 ccctcagcaa attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg
     7021 tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca
     7081 aagctcctat tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg
     7141 gtgctactcc taatggttca atttttacta ctttatattt atataattca cttctccaat
     7201 tgtccctcat atctcctcct ccaggtctga agatcagcgg ccggccgctt gctgtgcggt
     7261 ggtcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     7321 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     7381 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     7441 tgtttctaac aggccaggat taactgcgaa tcgttctagc tccctgcttg cccatactat
     7501 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     7561 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7621 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7681 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7741 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7801 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7861 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7921 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7981 tcgccactcc ccagtcccgc ccaggccacg cctccctgga aagtccccag cggaaagtcc
     8041 cttgtagcaa gctcgatatc agcagttctt gaagtactcc ggatgcagct ctcgggccac
     8101 gtgatgaaat gctaggcggc tgtcaaacct ccactctaac acttctctct ccgggtcatc
     8161 catcccatgc aggctcacag ggtgtaacaa gctggtgttc tctcctttat tggcctcttc
     8221 taccttatct ggctcaactg gtactagctt gtagcaccat ccaaaggtca gtggatatct
     8281 gacccctggc cctggtgtgt agttctgcta atcagggaag tagccttgtg tgtggtagat
     8341 ccacagatca aggatatctt gtcttctttg ggagtgaatt agcccttcca actactaagt
     8401 ttgtagtaca tatttaacaa atacaatttc tttaaaatga aaataattca gaggaatcac
     8461 aggtttagag taaatgaaac cacaggtaat tggcagtggt aatagggtat ggggtgggaa
     8521 gtttgggatg attttggtta gcttgagtta tccagttgat ccagacatga taagatacat
     8581 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     8641 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     8701 caattgcatt cattttatgt ttcaggttca gggggaggtg tgggaggttt tttaaagcaa
     8761 gtaaaacctc tacaaatgtg gtatggctga ttatgatctc tagtcaaggc actatacatc
     8821 aaatattcct tattaacccc tttacaaatt aaaaagctaa aggtacacaa tttttgagca
     8881 tagttattaa tagcagacac tctatgcctg tgtggagtaa gaaaaaacag tatgttatga
     8941 ttataactgt tatgcctact tataaaggtt acagaatatt tttccataat tttcttgtat
     9001 agcagtgcag ctttttcctt tgtggtgtaa atagcaaagc aagcaagagt tctattacta
     9061 aacacagcat gactcaaaaa acttagcaat tctgaaggaa agtccttggg gtcttctacc
     9121 tttctcttct tttttggagg agtagaatgt tgagagtcag cagtagcctc atcatcacta
     9181 gatggcattt cttctgagca aaacaggttt tcctcattaa aggcattcca ccactgctcc
     9241 cattcatcag ttccataggt tggaatctaa aatacacaaa caattagaat cagtagttta
     9301 acacattata cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt
     9361 ttgtccaatt atgtcacacc acagaagtaa ggttccttca caaagatccg gggcccactc
     9421 ataaatccag ttgccgccac ggtagccaat caccgtatcg tataaatcat cgtcggtacg
     9481 ttcggcatcg ctcatcacaa tacgtgcctg gacgtcgagg atttcgcgtg ggtcaatgcc
     9541 gcgccagatc cacatcagac ggttaatcat gcgataccag tgagggatgg ttttaccatc
     9601 aagggccgac tgcacaggcg gttgtgcgcc gtgattaaag cggcggacta gcgtcgaggt
     9661 ttcaggatgt ttaaagcggg gtttgaacag ggtttcgctc aggtttgcct gtgtcatgga
     9721 tgcagcctcc agaatactta ctggaaacta ttgtaacccg cctgaagtta aaaagaacaa
     9781 cgcccggcag tgccaggcgt tgaaaagatt agcgaccgga gattggcggg acgaatacga
     9841 cgcccatatc ccacggctgt tcaatccagg tatcttgcgg gatatcaaca acatagtcat
     9901 caaccagcgg acgaccagcc ggttttgcga agatggtgac aaagtgcgct tttggataca
     9961 tttcacgaat cgcaaccgca gtaccaccgg tatccaccag gtcatcaata acgatgaagc
    10021 cttcgccatc gccttctgcg cgtttcagca ctttaagctc gcgctggttg tcgtgatcgt
    10081 agctggaaat acaaacggta tcgacatgac gaatacccag ttcacgcgcc agtaacgcac
    10141 ccggtacaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
    10201 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
    10261 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat
    10321 aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc
    10381 gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc
    10441 gcaagcctgt cttgtgtcca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
    10501 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
    10561 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
    10621 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
    10681 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
    10741 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
    10801 acagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
//
