LOCUS       sequence-336499-       11267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagBFP-tagged Lck kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR Lck-TagBFP
FEATURES             Location/Qualifiers
     source          1..11267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     51..73
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(111..129)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        197..301
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(302..370,371..1162)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(520..539)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1333..1921
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1822..1841
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2075..2092
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2162..2182)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2167..2496
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2347..2482
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          3630..3695
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3825..3845
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4270..4404
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4307..4326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4361..4380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4572..5205
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    5252..5377
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5874..6107
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6292..6336
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6621..6738
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6891..7298
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             8906..9604
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    9702..10290
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9755..9775)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10173..10184)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(10293..10309)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10294..10310)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             10400..10633
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(10658..10676)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(10659..10676)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(10966..10985)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(11199..11218)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
       61 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
      121 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
      181 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      241 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      301 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      361 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      421 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      481 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      541 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      601 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      661 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      721 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      781 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      841 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      901 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      961 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     1021 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     1081 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1141 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1201 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1261 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1321 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1381 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1441 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1501 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1561 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1621 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1681 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1741 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1801 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1861 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1921 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1981 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     2041 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     2101 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2161 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     2221 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     2281 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     2341 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     2401 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     2461 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     2521 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     2581 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     2641 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     2701 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     2761 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     2821 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     2881 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     2941 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     3001 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     3061 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     3121 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     3181 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     3241 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     3301 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     3361 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     3421 cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga gcgatgccga
     3481 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     3541 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     3601 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     3661 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     3721 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     3781 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     3841 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     3901 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     3961 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     4021 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     4081 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     4141 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     4201 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     4261 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     4321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     4381 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     4441 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     4501 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     4561 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     4621 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     4681 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     4741 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     4801 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     4861 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     4921 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     4981 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     5041 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     5101 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     5161 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     5221 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     5281 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     5341 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     5401 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     5461 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     5521 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     5581 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     5641 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     5701 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     5761 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     5821 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     5881 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     5941 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     6001 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     6061 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     6121 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     6181 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
     6241 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
     6301 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
     6361 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
     6421 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
     6481 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
     6541 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
     6601 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     6661 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     6721 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
     6781 aagcttgata tcgaattcct gcagccccga taaaataaaa gattttattt agtctccaga
     6841 aaaagggggg aatgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat
     6901 tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta
     6961 catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg
     7021 cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga
     7081 tggtccccag atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg
     7141 ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc
     7201 tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac aacccctcac
     7261 tcggcgcgcc agtcctccga cagactgagt cgcccggggg ggatctggag ctctcgagaa
     7321 ttctcacgcg tcaagtggag caaggcaggt ggacagtgga tccatgggct gtggctgcag
     7381 ctcacacccg gaagatgact ggatggaaaa catcgatgtg tgtgagaact gccattatcc
     7441 catagtccca ctggatggca agggcacgct gctcatccga aatggctctg aggtgcggga
     7501 cccactgatt acctacgaag gctccaatcc gccggcttcc ccactgcaag acaacctggt
     7561 tatcgctctg cacagctatg agccctctca cgacggagat ctgggctttg agaaggggga
     7621 acagctccgc atcctggagc agagcggcga gtggtggaag gcgcagtccc tgaccacggg
     7681 ccaggaaggc ttcatcccct tcaattttgt ggccaaagcg aacagcctgg agcccgaacc
     7741 ctggttcttc aagaacctga gccgcaagga cgcggagcgg cagctcctgg cgcccgggaa
     7801 cactcacggc tccttcctca tccgggagag cgagagcacc gcgggatcgt tttcactgtc
     7861 ggtccgggac ttcgaccaga accagggaga ggtggtgaaa cattacaaga tccgtaatct
     7921 ggacaacggt ggcttctaca tctcccctcg aatcactttt cccggcctgc atgaactggt
     7981 ccgccattac accaatgctt cagatgggct gtgcacacgg ttgagccgcc cctgccagac
     8041 ccagaagccc cagaagccgt ggtgggagga cgagtgggag gttcccaggg agacgctgaa
     8101 gctggtggag cggctggggg ctggacagtt cggggaggtg tggatggggt actacaacgg
     8161 gcacacgaag gtggcggtga agagcctgaa gcagggcagc atgtccccgg acgccttcct
     8221 ggccgaggcc aacctcatga agcagctgca acaccagcgg ctggttcggc tctacgctgt
     8281 ggtcacccag gagcccatct acatcatcac tgaatacatg gagaatggga gtctagtgga
     8341 ttttctcaag accccttcag gcatcaagtt gaccatcaac aaactcctgg acatggcagc
     8401 ccaaattgca gaaggcatgg cattcattga agagcggaat tatattcatc gtgaccttcg
     8461 ggctgccaac attctggtgt ctgacaccct gagctgcaag attgcagact ttggcctagc
     8521 acgcctcatt gaggacaacg agtacacagc cagggagggg gccaagtttc ccattaagtg
     8581 gacagcgcca gaagccatta actacgggac attcaccatc aagtcagatg tgtggtcttt
     8641 tgggatcctg ctgacggaaa ttgtcaccca cggccgcatc ccttacccag ggatgaccaa
     8701 cccggaggtg attcagaacc tggagcgagg ctaccgcatg gtgcgccctg acaactgtcc
     8761 agaggagctg taccaactca tgaggctgtg ctggaaggag cgcccagagg accggcccac
     8821 ctttgactac ctgcgcagtg tgctggagga cttcttcacg gccacagagg gccagtacca
     8881 gcctcagcct ggatccggag ctaccatgag cgagctgatt aaggagaaca tgcacatgaa
     8941 gctgtacatg gagggcaccg tggacaacca tcacttcaag tgcacatccg agggcgaagg
     9001 caagccctac gagggcaccc agaccatgag aatcaaggtg gtcgagggcg gccctctccc
     9061 cttcgccttc gacatcctgg ctactagctt cctctacggc agcaagacct tcatcaacca
     9121 cacccagggc atccccgact tcttcaagca gtccttccct gagggcttca catgggagag
     9181 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     9241 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcacatcca acggccctgt
     9301 gatgcagaag aaaacactcg gctgggaggc cttcaccgag acgctgtacc ccgctgacgg
     9361 cggcctggaa ggcagaaacg acatggccct gaagctcgtg ggcgggagcc atctgatcgc
     9421 aaacatcaag accacatata gatccaagaa acccgctaag aacctcaaga tgcctggcgt
     9481 ctactatgtg gactacagac tggaaagaat caaggaggcc aacaacgaga cctacgtcga
     9541 gcagcacgag gtggcagtgg ccagatactg cgacctccct agcaaactgg ggcacaagct
     9601 taatgcggcc gcaactccca cctgcaacat gcgtgactga ctgaggccgc gactctagag
     9661 tcgacctgca ggcatgcaag cttgatatca agcttatcga taatcaacct ctggattaca
     9721 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     9781 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     9841 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     9901 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     9961 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
    10021 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
    10081 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
    10141 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
    10201 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
    10261 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga gggaattaat
    10321 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
    10381 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
    10441 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
    10501 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
    10561 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
    10621 aaaatctcta gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta
    10681 tgtgtatgat acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca
    10741 agcctaattg tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg
    10801 ccgtcagagt cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc
    10861 cggaaatggt cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac
    10921 gcatcgtggc cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca
    10981 tcaccgatgg ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg
    11041 gtatggtggc aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat
    11101 tccttgcggc ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg
    11161 agtcgcataa gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca
    11221 tagttaagcc agccccgaca cccgccaaca cccgctgacg cgccctg
//
