LOCUS       sequence-336502-       11351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagBFP-tagged Fyn kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11351)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 11351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR Fyn-TagBFP
FEATURES             Location/Qualifiers
     source          1..11351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          1234..1299
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1429..1449
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    1874..2008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1911..1930)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1965..1984
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2176..2809
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2856..2981
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3478..3711
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3896..3940
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4225..4342
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4495..4902
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             6594..7292
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    7390..7978
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7443..7463)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7861..7872)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7981..7997)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7982..7998)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8088..8321
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8346..8364)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8347..8364)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(8654..8673)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8887..8906)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9006..9028
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9066..9084)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9152..9256
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9257..9325,9326..10117)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9475..9494)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10288..10876
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10777..10796
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11030..11047
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11117..11137)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        join(11122..11351,1..100)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      join(11302..11351,1..86)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa
       61 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagcttggac acaagacagg
      121 cttgcgagat atgtttgaga ataccacttt atcccgcgtc agggagaggc agtgcgtaaa
      181 aagacgcgga ctcatgtgaa atactggttt ttagtgcgcc agatctctat aatctcgcgc
      241 aacctatttt cccctcgaac actttttaag ccgtagataa acaggctggg acacttcaca
      301 tgagcgaaaa atacatcgtc acctgggaca tgttgcagat ccatgcacgt aaactcgcaa
      361 gccgactgat gccttctgaa caatggaaag gcattattgc cgtaagccgt ggcggtctgt
      421 accgggtgcg ttactggcgc gtgaactggg tattcgtcat gtcgataccg tttgtatttc
      481 cagctacgat cacgacaacc agcgcgagct taaagtgctg aaacgcgcag aaggcgatgg
      541 cgaaggcttc atcgttattg atgacctggt ggataccggt ggtactgcgg ttgcgattcg
      601 tgaaatgtat ccaaaagcgc actttgtcac catcttcgca aaaccggctg gtcgtccgct
      661 ggttgatgac tatgttgttg atatcccgca agatacctgg attgaacagc cgtgggatat
      721 gggcgtcgta ttcgtcccgc caatctccgg tcgctaatct tttcaacgcc tggcactgcc
      781 gggcgttgtt ctttttaact tcaggcgggt tacaatagtt tccagtaagt attctggagg
      841 ctgcatccat gacacaggca aacctgagcg aaaccctgtt caaaccccgc tttaaacatc
      901 ctgaaacctc gacgctagtc cgccgcttta atcacggcgc acaaccgcct gtgcagtcgg
      961 cccttgatgg taaaaccatc cctcactggt atcgcatgat taaccgtctg atgtggatct
     1021 ggcgcggcat tgacccacgc gaaatcctcg acgtccaggc acgtattgtg atgagcgatg
     1081 ccgaacgtac cgacgatgat ttatacgata cggtgattgg ctaccgtggc ggcaactgga
     1141 tttatgagtg ggccccggat ctttgtgaag gaaccttact tctgtggtgt gacataattg
     1201 gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt aagtgtataa
     1261 tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta tggaactgat
     1321 gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc agaagaaatg
     1381 ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc aaaaaagaag
     1441 agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt gagtcatgct
     1501 gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga aaaagctgca
     1561 ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag gcataacagt
     1621 tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc tgctattaat
     1681 aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt taataaggaa
     1741 tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt
     1801 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
     1861 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     1921 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     1981 catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc aaaatcatcc
     2041 caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc atttactcta
     2101 aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt aaatatgtac
     2161 tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat atccttgatc
     2221 tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca ccagggccag
     2281 gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt gagccagata
     2341 aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg agcctgcatg
     2401 ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc ctagcatttc
     2461 atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat cgagcttgct
     2521 acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg actggggagt
     2581 ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg gtctctctgg
     2641 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     2701 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     2761 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga
     2821 acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg actcggcttg
     2881 ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca aaaattttga
     2941 ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag cgggggagaa
     3001 ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa tataaattaa
     3061 aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct ggcctgttag
     3121 aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt cagacaggat
     3181 cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg catcaaagga
     3241 tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa aacaaaagta
     3301 agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag gagatatgag
     3361 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     3421 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     3481 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     3541 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     3601 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     3661 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     3721 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     3781 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     3841 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     3901 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     3961 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     4021 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     4081 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     4141 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     4201 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     4261 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4321 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cagtttggat
     4381 cgataagctt gatatcgaat tcctgcagcc ccgataaaat aaaagatttt atttagtctc
     4441 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     4501 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     4561 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     4621 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     4681 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     4741 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     4801 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     4861 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggggggatct ggagctctcg
     4921 agaattctca cgcgtcaagt ggagcaaggc aggtggacag tggatccatg ggctgtgtgc
     4981 aatgtaagga taaagaagca acaaaactga cggaggagag ggacggcagc ctgaaccaga
     5041 gctctgggta ccgctatggc acagacccca cccctcagca ctaccccagc ttcggtgtga
     5101 cctccatccc caactacaac aacttccacg cagccggggg ccaaggactc accgtctttg
     5161 gaggtgtgaa ctcttcgtct catacgggga ccttgcgtac gagaggagga acaggagtga
     5221 cactctttgt ggccctttat gactatgaag cacggacaga agatgacctg agttttcaca
     5281 aaggagaaaa atttcaaata ttgaacagct cggaaggaga ttggtgggaa gcccgctcct
     5341 tgacaactgg agagacaggt tacattccca gcaattatgt ggctccagtt gactctatcc
     5401 aggcagaaga gtggtacttt ggaaaacttg gccgaaaaga tgctgagcga cagctattgt
     5461 cctttggaaa cccaagaggt acctttctta tccgcgagag tgaaaccacc aaaggtgcct
     5521 attcactttc tatccgtgat tgggatgata tgaaaggaga ccatgtcaaa cattataaaa
     5581 ttcgcaaact tgacaatggt ggatactaca ttaccacccg ggcccagttt gaaacacttc
     5641 agcagcttgt acaacattac tcagagagag ctgcaggtct ctgctgccgc ctagtagttc
     5701 cctgtcacaa agggatgcca aggcttaccg atctgtctgt caaaaccaaa gatgtctggg
     5761 aaatccctcg agaatccctg cagttgatca agagactggg aaatgggcag tttggggaag
     5821 tatggatggg tacctggaat ggaaacacaa aagtagccat aaagactctt aaaccaggca
     5881 caatgtcccc cgaatcattc cttgaggaag cgcagatcat gaagaagctg aagcacgaca
     5941 agctggtcca gctctatgca gtggtgtctg aggagcccat ctacatcgtc accgagtata
     6001 tgaacaaagg aagtttactg gatttcttaa aagatggaga aggaagagct ctgaaattac
     6061 caaatcttgt ggacatggca gcacaggtgg ctgcaggaat ggcttacatc gagcgcatga
     6121 attatatcca tagagatctg cgatcagcaa acattctagt ggggaatgga ctcatatgca
     6181 agattgctga cttcggattg gcccgattga tagaagacaa tgagtacaca gcaagacaag
     6241 gtgcaaagtt ccccatcaag tggacggccc ccgaggcagc cctgtacggg aggttcacaa
     6301 tcaagtctga cgtgtggtct tttggaatct tactcacaga gctggtcacc aaaggaagag
     6361 tgccataccc aggcatgaac aaccgggagg tgctggagca ggtggagcga ggctacagga
     6421 tgccctgccc gcaggactgc cccatctctc tgcatgagct catgatccac tgctggaaaa
     6481 aggaccctga agaacgcccc acttttgagt acttgcagag cttcctggaa gactacttta
     6541 ccgcgacaga gccccagtac caacctggtg aaaacctggg atccggagct accatgagcg
     6601 agctgattaa ggagaacatg cacatgaagc tgtacatgga gggcaccgtg gacaaccatc
     6661 acttcaagtg cacatccgag ggcgaaggca agccctacga gggcacccag accatgagaa
     6721 tcaaggtggt cgagggcggc cctctcccct tcgccttcga catcctggct actagcttcc
     6781 tctacggcag caagaccttc atcaaccaca cccagggcat ccccgacttc ttcaagcagt
     6841 ccttccctga gggcttcaca tgggagagag tcaccacata cgaagacggg ggcgtgctga
     6901 ccgctaccca ggacaccagc ctccaggacg gctgcctcat ctacaacgtc aagatcagag
     6961 gggtgaactt cacatccaac ggccctgtga tgcagaagaa aacactcggc tgggaggcct
     7021 tcaccgagac gctgtacccc gctgacggcg gcctggaagg cagaaacgac atggccctga
     7081 agctcgtggg cgggagccat ctgatcgcaa acatcaagac cacatataga tccaagaaac
     7141 ccgctaagaa cctcaagatg cctggcgtct actatgtgga ctacagactg gaaagaatca
     7201 aggaggccaa caacgagacc tacgtcgagc agcacgaggt ggcagtggcc agatactgcg
     7261 acctccctag caaactgggg cacaagctta atgcggccgc aactcccacc tgcaacatgc
     7321 gtgactgact gaggccgcga ctctagagtc gacctgcagg catgcaagct tgatatcaag
     7381 cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac
     7441 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt
     7501 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat
     7561 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca
     7621 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc
     7681 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg
     7741 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct
     7801 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct
     7861 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt
     7921 ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat
     7981 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
     8041 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     8101 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     8161 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     8221 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     8281 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
     8341 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
     8401 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
     8461 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
     8521 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
     8581 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
     8641 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
     8701 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
     8761 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
     8821 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
     8881 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
     8941 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
     9001 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
     9061 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     9121 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     9181 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     9241 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     9301 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     9361 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     9421 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9481 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9541 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9601 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9661 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9721 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9781 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9841 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9901 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9961 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
    10021 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
    10081 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
    10141 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
    10201 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
    10261 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
    10321 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
    10381 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
    10441 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
    10501 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
    10561 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
    10621 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
    10681 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
    10741 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
    10801 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
    10861 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
    10921 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
    10981 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
    11041 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
    11101 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
    11161 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
    11221 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
    11281 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    11341 ccccatggct g
//
