LOCUS       sequence-336503-       11345 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagBFP-tagged Src kinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11345)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 11345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR Src-TagBFP
FEATURES             Location/Qualifiers
     source          1..11345
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             249..293
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    578..695
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        848..1255
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             2941..3639
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    3737..4325
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3790..3810)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4208..4219)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4328..4344)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4329..4345)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4435..4668
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4693..4711)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4694..4711)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5001..5020)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5234..5253)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5353..5375
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5413..5431)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5499..5603
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5604..5672,5673..6464)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5822..5841)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6635..7223
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7124..7143
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7377..7394
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7464..7484)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7469..7798
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7649..7784
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8932..8997
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9127..9147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9572..9706
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9609..9628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9663..9682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             9874..10507
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    10554..10679
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(11176..11345,1..64)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
       61 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
      121 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
      181 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
      241 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
      301 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
      361 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
      421 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
      481 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
      541 ctcgacggta tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
      601 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
      661 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
      721 atccagtttg gatcgataag cttgatatcg aattcctgca gccccgataa aataaaagat
      781 tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct
      841 agctgcagta acgccatttt gcaaggcatg gaaaaatacc aaaccaagaa tagagaagtt
      901 cagatcaagg gcgggtacat gaaaatagct aacgttgggc caaacaggat atctgcggtg
      961 agcagtttcg gccccggccc ggggccaaga acagatggtc accgcagttt cggccccggc
     1021 ccgaggccaa gaacagatgg tccccagata tggcccaacc ctcagcagtt tcttaagacc
     1081 catcagatgt ttccaggctc ccccaaggac ctgaaatgac cctgcgcctt atttgaatta
     1141 accaatcagc ctgcttctcg cttctgttcg cgcgcttctg cttcccgagc tctataaaag
     1201 agctcacaac ccctcactcg gcgcgccagt cctccgacag actgagtcgc ccggggggga
     1261 tctggagctc tcgagaattc tcacgcgtca agtggagcaa ggcaggtgga cagtggatcc
     1321 atgggcagca acaagagcaa gcccaaggac gccagccagc ggcgccgcag cctggagccc
     1381 tcggaaaacg tgcacggggc agggggcgcc ttcccggcct cacagacacc gagcaagccc
     1441 gcctccgccg acggccaccg cgggcccagc gccgccttcg tgccgcccgc ggccgagccc
     1501 aagctcttcg gaggcttcaa ctcctcggac accgtcacct ccccgcagag ggcggggcct
     1561 ctggcaggtg gggtgaccac ctttgtggcc ctctatgact atgagtcacg gacagagact
     1621 gacctgtcct tcaagaaagg ggagcggctg cagattgtca ataacacgga gggagactgg
     1681 tggctggcac actcgctgag cacgggacag accggttaca tccccagcaa ctatgtggcg
     1741 ccctccgact ccatccaggc tgaggagtgg tactttggca agatcactag acgggaatca
     1801 gagcggctgc tgctcaacgc cgagaacccg agagggacct tcctcgtgag ggagagtgag
     1861 accacaaaag gtgcctactg cctctctgta tccgacttcg acaatgccaa gggcctaaat
     1921 gtgaaacact acaagatccg caagctggac agcggcggtt tctacatcac ctcccgcacc
     1981 cagttcaaca gcctgcagca gctcgtggct tactactcca aacatgctga tggcctgtgt
     2041 caccgcctca ctaccgtatg tcccacatcc aagcctcaga cccagggatt ggccaaggat
     2101 gcgtgggaga tcccccggga gtccctgcgg ctggaggtca agctgggcca gggttgcttc
     2161 ggagaggtgt ggatggggac ctggaacggc accacgaggg ttgccatcaa gactctgaag
     2221 ccaggcacca tgtccccaga ggccttcctg caggaggccc aagtcatgaa gaaactgagg
     2281 cacgagaaac tggtgcagct gtatgctgtg gtgtcggaag aacccattta cattgtgaca
     2341 gagtacatga acaaggggag tctgctggac tttctcaagg gggaaacggg caaatatttg
     2401 cggctacccc agctggtgga catgtctgct cagatcgctt caggcatggc ctatgtggag
     2461 cggatgaact atgtgcaccg ggaccttcga gccgccaata tcctagtagg ggagaacctg
     2521 gtgtgcaaag tggccgactt tgggttggcc cggctcatag aagacaacga atacacagcc
     2581 cggcaaggtg ccaaattccc catcaagtgg accgcccctg aagctgctct gtacggcagg
     2641 ttcaccatca agtcggatgt gtggtccttt gggattctgc tgaccgagct caccactaag
     2701 ggaagagtgc cctatcctgg gatggtgaac cgtgaggttc tggaccaggt ggagcggggc
     2761 taccggatgc cttgtccccc cgagtgcccc gagtccctgc atgaccttat gtgccagtgc
     2821 tggcggaagg agcccgagga gcggcccacc ttcgagtacc tgcaggcctt cctggaagac
     2881 tactttacgt ccactgagcc acagtaccag cccggggaga acctaggatc cggagctacc
     2941 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3001 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3061 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3121 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3181 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     3241 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     3301 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     3361 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     3421 gccctgaagc tcgtgggcgg gagccatctg atcgcaaaca tcaagaccac atatagatcc
     3481 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     3541 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     3601 tactgcgacc tccctagcaa actggggcac aagcttaatg cggccgcaac tcccacctgc
     3661 aacatgcgtg actgactgag gccgcgactc tagagtcgac ctgcaggcat gcaagcttga
     3721 tatcaagctt atcgataatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3781 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3841 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3901 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3961 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     4021 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     4081 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc
     4141 ctttccttgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     4201 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     4261 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     4321 cccgcatcga taccgtcgac ctcgagggaa ttaattcgag ctcggtacct ttaagaccaa
     4381 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     4441 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     4501 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     4561 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     4621 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagc atctagaatt
     4681 aattccgtgt attctatagt gtcacctaaa tcgtatgtgt atgatacata aggttatgta
     4741 ttaattgtag ccgcgttcta acgacaatat gtacaagcct aattgtgtag catctggctt
     4801 actgaagcag accctatcat ctctctcgta aactgccgtc agagtcggtt tggttggacg
     4861 aaccttctga gtttctggta acgccgtccc gcacccggaa atggtcagcg aaccaatcag
     4921 cagggtcatc gctagccaga tcctctacgc cggacgcatc gtggccggca tcaccggcgc
     4981 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg
     5041 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg
     5101 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg
     5161 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgaat
     5221 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc
     5281 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     5341 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     5401 cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
     5461 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     5521 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5581 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5641 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5701 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5761 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5821 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5881 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5941 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     6001 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     6061 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     6121 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     6181 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     6241 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     6301 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     6361 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     6421 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     6481 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6541 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6601 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6661 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6721 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6781 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6841 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6901 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6961 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7021 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7081 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7141 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7201 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7261 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7321 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7381 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     7441 ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg
     7501 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg
     7561 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     7621 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     7681 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     7741 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     7801 gcttggacac aagacaggct tgcgagatat gtttgagaat accactttat cccgcgtcag
     7861 ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat actggttttt agtgcgccag
     7921 atctctataa tctcgcgcaa cctattttcc cctcgaacac tttttaagcc gtagataaac
     7981 aggctgggac acttcacatg agcgaaaaat acatcgtcac ctgggacatg ttgcagatcc
     8041 atgcacgtaa actcgcaagc cgactgatgc cttctgaaca atggaaaggc attattgccg
     8101 taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt
     8161 cgataccgtt tgtatttcca gctacgatca cgacaaccag cgcgagctta aagtgctgaa
     8221 acgcgcagaa ggcgatggcg aaggcttcat cgttattgat gacctggtgg ataccggtgg
     8281 tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa
     8341 accggctggt cgtccgctgg ttgatgacta tgttgttgat atcccgcaag atacctggat
     8401 tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt
     8461 tcaacgcctg gcactgccgg gcgttgttct ttttaacttc aggcgggtta caatagtttc
     8521 cagtaagtat tctggaggct gcatccatga cacaggcaaa cctgagcgaa accctgttca
     8581 aaccccgctt taaacatcct gaaacctcga cgctagtccg ccgctttaat cacggcgcac
     8641 aaccgcctgt gcagtcggcc cttgatggta aaaccatccc tcactggtat cgcatgatta
     8701 accgtctgat gtggatctgg cgcggcattg acccacgcga aatcctcgac gtccaggcac
     8761 gtattgtgat gagcgatgcc gaacgtaccg acgatgattt atacgatacg gtgattggct
     8821 accgtggcgg caactggatt tatgagtggg ccccggatct ttgtgaagga accttacttc
     8881 tgtggtgtga cataattgga caaactacct acagagattt aaagctctaa ggtaaatata
     8941 aaatttttaa gtgtataatg tgttaaacta ctgattctaa ttgtttgtgt attttagatt
     9001 ccaacctatg gaactgatga atgggagcag tggtggaatg cctttaatga ggaaaacctg
     9061 ttttgctcag aagaaatgcc atctagtgat gatgaggcta ctgctgactc tcaacattct
     9121 actcctccaa aaaagaagag aaaggtagaa gaccccaagg actttccttc agaattgcta
     9181 agttttttga gtcatgctgt gtttagtaat agaactcttg cttgctttgc tatttacacc
     9241 acaaaggaaa aagctgcact gctatacaag aaaattatgg aaaaatattc tgtaaccttt
     9301 ataagtaggc ataacagtta taatcataac atactgtttt ttcttactcc acacaggcat
     9361 agagtgtctg ctattaataa ctatgctcaa aaattgtgta cctttagctt tttaatttgt
     9421 aaaggggtta ataaggaata tttgatgtat agtgccttga ctagagatca taatcagcca
     9481 taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc ccctgaacct
     9541 gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt ataatggtta
     9601 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     9661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca
     9721 agctaaccaa aatcatccca aacttcccac cccataccct attaccactg ccaattacct
     9781 gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg
     9841 tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa
     9901 gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga
     9961 actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag
    10021 taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac
    10081 accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg
    10141 acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact
    10201 gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc
    10261 tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc
    10321 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    10381 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
    10441 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
    10501 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
    10561 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
    10621 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
    10681 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
    10741 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
    10801 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
    10861 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
    10921 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
    10981 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc
    11041 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
    11101 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
    11161 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
    11221 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
    11281 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
    11341 ctggg
//
