LOCUS       sequence-336505-       15471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is 
            TagRFP-labeled..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15471)
  AUTHORS   Dine E, Reed EH, Toettcher JE
  TITLE     Positive feedback between the T cell kinase Zap70 and its substrate 
            LAT acts as a clustering-dependent signaling switch.
  JOURNAL   Cell Rep. 2021 Jun 22;35(12):109280. doi: 
            10.1016/j.celrep.2021.109280.
  PUBMED    34161759
REFERENCE   2  (bases 1 to 15471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-06-22"; volume: "35(12):109280. doi"; pages: " 
            10.1016/j.celrep.2021.109280"
COMMENT     Sequence Label: pHR LAT/Zap70 iLID-Drop
FEATURES             Location/Qualifiers
     source          1..15471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             219..263
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    548..665
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        818..1225
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             1291..2022
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             2098..2427
                     /codon_start=1
                     /product="SspB from Haemophilus influenzae, with the 
                     mutations Y11K and A15E to disrupt the natural dimerization
                     of the protein"
                     /label=SspB nano
                     /note="dimerizes with iLID at high affinity to create iLID 
                     nano (Guntas et al., 2015)"
                     /translation="SSPKRPKLLREYYDWLVDNSFTPYLVVDATYLGVNVPVEYVKDGQ
                     IVLNLSASATGNLQLTNDFIQFNARFKGVSRELYIPMGAALAIYARENGDGVMFEPEEI
                     YDELNI"
     CDS             4318..4374
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5080..5517
                     /codon_start=1
                     /product="improved light-inducible dimer when used with 
                     SspB nano or SspB micro (Guntas et al., 2015)"
                     /label=iLID
                     /translation="LATTLERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGR
                     NCRFLQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQ
                     YFIGVQLDGTERLHGAAEREAVCLIKKTAFQIAEAANDENYF"
     CDS             5937..5948
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             6208..7701
                     /codon_start=1
                     /product="N-terminal photolyase homology region of 
                     Arabidopsis thaliana cryptochrome 2"
                     /label=CRY2PHR
                     /note="binds to CIB1 upon exposure to blue light (Kennedy 
                     et al., 2010)"
                     /translation="MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQ
                     FYPGRASRWWMKQSLAHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLY
                     DPVSLVRDHTVKEKLVERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSI
                     ESVMLPPPWRLMPITAAAEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLN
                     EFIEKQLIDYAKNSKKVVGNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGE
                     ESADLFLRGIGLREYSRYICFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPL
                     VDAGMRELWATGWMHNRIRVIVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQY
                     ISGSIPDGHELDRLDNPALQGAKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKA
                     SGVELGTNYAKPIVDIDTARELLAKAISRTREAQIMIGAA"
     misc_feature    7833..8421
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7886..7906)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8304..8315)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(8424..8440)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8425..8441)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8531..8764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(8789..8807)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(8790..8807)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(9097..9116)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(9330..9349)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9449..9471
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9509..9527)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9595..9699
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9700..9768,9769..10560)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9918..9937)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10731..11319
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11220..11239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11473..11490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(11560..11580)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11565..11894
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11745..11880
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          13028..13093
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             13223..13243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    13668..13802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(13705..13724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     13759..13778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             13970..14603
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    14650..14775
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    join(15272..15471,1..34)
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
       61 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
      121 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
      181 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
      241 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
      301 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
      361 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
      421 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
      481 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgccaaatg gcagtattca
      541 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
      601 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
      661 attttcgggt ttattacagg gacagcagag atccagtttg gatcgataag cttgatatcg
      721 aattcctgca gccccgataa aataaaagat tttatttagt ctccagaaaa aggggggaat
      781 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
      841 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
      901 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
      961 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
     1021 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
     1081 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
     1141 cgcgcttctg cttcccgagc tctataaaag agctcacaac ccctcactcg gcgcgccagt
     1201 cctccgacag actgagtcgc ccggggggga tctggagctc tcgagaattc tcacgcgtca
     1261 agtggagcaa ggcaggtgga cagtggatcc atggtgtcta agggcgaaga gctgattaag
     1321 gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca cttcaagtgc
     1381 acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat caaggtggtc
     1441 gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat gtacggcagc
     1501 agaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc cttccctgag
     1561 ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac cgctacccag
     1621 gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg ggtgaacttc
     1681 ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggccaa caccgagatg
     1741 ctgtaccccg ctgacggcgg cctggaaggc agaaccgaca tggccctgaa gctcgtgggc
     1801 gggggccacc tgatctgcaa cttcaagacc acatacagat ccaagaaacc cgctaagaac
     1861 ctcaagatgc ccggcgtcta ctatgtggac cacagactgg aaagaatcaa ggaggccgac
     1921 aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga cctccctagc
     1981 aaactggggc acaaacttaa tggcatggac gagctgtaca agtccggact cagatctcga
     2041 gctcaagctt cgaacgaatt cggcattgat ctgagcggcc tgaccctgca ggaattcagc
     2101 tccccgaaac gccctaagct gctgcgtgaa tattacgatt ggctggttga taacagcttt
     2161 accccatatc tggtggtgga tgccacatac ctgggcgtga acgtgcccgt ggagtatgtg
     2221 aaagacggtc agatcgtgct gaatctgtct gcaagtgcga ccggcaacct gcaactgaca
     2281 aatgatttta tccagttcaa cgcccgcttt aagggcgtgt ctcgtgaact gtatatcccg
     2341 atgggtgccg ctctggccat ttacgctcgc gagaacggcg atggtgtgat gttcgaacca
     2401 gaagaaatct atgacgagct gaatattggt agtgctggtg gtgaattcat gatgccagac
     2461 cccgcggcgc acctgccctt cttctacggc agcatctcgc gtgccgaggc cgaggagcac
     2521 ctgaagctgg cgggcatggc ggacgggctc ttcctgctgc gccagtgcct gcgctcgctg
     2581 ggcggctatg tgctgtcgct cgtgcacgat gtgcgcttcc accactttcc catcgagcgc
     2641 cagctcaacg gcacctacgc cattgccggc ggcaaagcgc actgtggacc ggcagagctc
     2701 tgcgagttct actcgcgcga ccccgacggg ctgccctgca acctgcgcaa gccgtgcaac
     2761 cggccgtcgg gcctcgagcc gcagccgggg gtcttcgact gcctgcgaga cgccatggtg
     2821 cgtgactacg tgcgccagac gtggaagctg gagggcgagg ccctggagca ggccatcatc
     2881 agccaggccc cgcaggtgga gaagctcatt gctacgacgg cccacgagcg gatgccctgg
     2941 taccacagca gcctgacgcg tgaggaggcc gagcgcaaac tttactctgg ggcgcagacc
     3001 gacggcaagt tcctgctgag gccgcggaag gagcagggca catacgccct gtccctcatc
     3061 tatgggaaga cggtgtacca ctacctcatc agccaagaca aggcgggcaa gtactgcatt
     3121 cccgagggca ccaagtttga cacgctctgg cagctggtgg agtatctgaa gctgaaggcg
     3181 gacgggctca tctactgcct gaaggaggcc tgccccaaca gcagtgccag caacgcctca
     3241 ggggctgctg ctcccacact cccagcccac ccatccacgt tgactcatcc tcagagacga
     3301 atcgacaccc tcaactcaga tggatacacc cctgagccag cacgcataac gtccccagac
     3361 aaaccgcggc cgatgcccat ggacacgagc gtgtatgaga gcccctacag cgacccagag
     3421 gagctcaagg acaagaagct cttcctgaag cgcgataacc tcctcatagc tgacattgaa
     3481 cttggctgcg gcaactttgg ctcagtgcgc cagggcgtgt accgcatgcg caagaagcag
     3541 atcgacgtgg ccatcaaggt gctgaagcag ggcacggaga aggcagacac ggaagagatg
     3601 atgcgcgagg cgcagatcat gcaccagctg gacaacccct acatcgtgcg gctcattggc
     3661 gtctgccagg ccgaggccct catgctggtc atggagatgg ctgggggcgg gccgctgcac
     3721 aagttcctgg tcggcaagag ggaggagatc cctgtgagca atgtggccga gctgctgcac
     3781 caggtgtcca tggggatgaa gtacctggag gagaagaact ttgtgcaccg tgacctggcg
     3841 gcccgcaacg tcctgctggt taaccggcac tacgccaaga tcagcgactt tggcctctcc
     3901 aaagcactgg gtgccgacga cagctactac actgcccgct cagcagggaa gtggccgctc
     3961 aagtggtacg cacccgaatg catcaacttc cgcaagttct ccagccgcag cgatgtctgg
     4021 agctatgggg tcaccatgtg ggaggccttg tcctacggcc agaagcccta caagaagatg
     4081 aaagggccgg aggtcatggc cttcatcgag cagggcaagc ggatggagtg cccaccagag
     4141 tgtccacccg aactgtacgc actcatgagt gactgctgga tctacaagtg ggaggatcgc
     4201 cccgacttcc tgaccgtgga gcagcgcatg cgagcctgtt actacagcct ggccagcaag
     4261 gtggaagggc ccccaggcag cacacagaag gctgaggctg cctgtgccgg aagcggagct
     4321 actaacttca gcctgctgaa gcaggctgga gacgtggagg agaaccctgg acctatggag
     4381 gaggccatcc tggtcccctg cgtgctgggg ctcctgctgc tgcccatcct ggccatgttg
     4441 atggcactgt gtgtgcactg ccacagactg ccaggctcct acgacagcac atcctcagat
     4501 agtttgtatc caaggggcat ccagttcaaa cggcctcaca cggttgcccc ctggccacct
     4561 gcctacccac ctgtcacctc ctacccaccc ctgagccagc cagacctgct ccccatccca
     4621 agatccccgc agccccttgg gggctcccac cggacgccat cttcccggcg ggattctgat
     4681 ggtgccaaca gtgtggcgag ctacgagaac gaggaaccag cctgtgagga tgcggatgag
     4741 gatgaggacg actatcacaa cccaggctac ctggtggtgc ttcctgacag caccccggcc
     4801 actagcactg ctgccccatc agctcctgca ctcagcaccc ctggcatccg agacagtgcc
     4861 ttctccatgg agtccattga tgattacgtg aacgttccgg agagcgggga gagcgcagaa
     4921 gcgtctctgg atggcagccg ggagtatgtg aatgtgtccc aggaactgca tcctggagcg
     4981 gctaagactg agcctgccgc cctgagttcc caggaggcag aggaagtgga ggaagagggg
     5041 gctccagatt acgagaatct gcaggagctg aacgagtttc tggcaaccac actggaacgg
     5101 atcgagaaaa atttcgtgat tactgatccg agactgcctg acaacccaat catttttgcg
     5161 agcgattcct tcctgcagct gacagaatat tctcgggaag agatcctggg gcgcaattgc
     5221 cgttttctgc agggacccga gacagaccgt gccactgttc ggaaaatcag agatgctatt
     5281 gacaaccaga ctgaagtgac cgttcagctg atcaattata ccaagagcgg caagaagttc
     5341 tggaacgtgt tccacctgca gccgatgcgc gattataagg gcgacgtcca gtacttcatt
     5401 ggcgtgcagc tggatggcac cgaacgtctt catggcgccg ctgagcgtga ggcggtctgc
     5461 ctgatcaaaa agacagcctt tcagattgct gaggcagcga acgacgaaaa ttactttggt
     5521 tcagggagcg gatccggctc agggagtgcg gccgcaatgg cctcaaacga ttatacccaa
     5581 caagcaaccc aaagctatgg ggcctacccc acccagcccg ggcagggcta ttcccagcag
     5641 agcagtcagc cctacggaca gcagagttac agtggttata gccagtccac ggacacttca
     5701 ggctatggcc agagcagcta ttcttcttat ggccagagcc agaacacagg ctatggaact
     5761 cagtcaactc cccagggata tggctcgact ggcggctatg gcagtagcca gagctcccaa
     5821 tcgtcttacg ggcagcagtc ctcctatcct ggctatggcc agcagccagc tcccagcagc
     5881 acctcgggaa gttacggtag cagttctcag agcagcagct atgggcagcc ccagagtggg
     5941 agctacagcc agcagcctag ctatggtgga cagcagcaaa gctatggaca gcagcaaagc
     6001 tataatcccc ctcagggcta tggacagcag aaccagtaca acagcagcag tggtggtgga
     6061 ggtggaggtg gaggtggagg taactatggc caagatcaat cctccatgag tagtggtggt
     6121 ggcagtggtg gcggttatgg caatcaagac cagagtggtg gaggtggcag cggtggctat
     6181 ggacagcagg accgtggagg tggaggaatg aagatggaca aaaagactat agtttggttt
     6241 agaagagacc taaggattga ggataatcct gcattagcag cagctgctca cgaaggatct
     6301 gtttttcctg tcttcatttg gtgtcctgaa gaagaaggac agttttatcc tggaagagct
     6361 tcaagatggt ggatgaaaca atcacttgct cacttatctc aatccttgaa ggctcttgga
     6421 tctgacctca ctttaatcaa aacccacaac acgatttcag cgatcttgga ttgtatccgc
     6481 gttaccggtg ctacaaaagt cgtctttaac cacctctatg atcctgtttc gttagttcgg
     6541 gaccataccg taaaggagaa gctggtggaa cgtgggatct ctgtgcaaag ctacaatgga
     6601 gatctattgt atgaaccgtg ggagatatac tgcgaaaagg gcaaaccttt tacgagtttc
     6661 aattcttact ggaagaaatg cttagatatg tcgattgaat ccgttatgct tcctcctcct
     6721 tggcggttga tgccaataac tgcagcggct gaagcgattt gggcgtgttc gattgaagaa
     6781 ctagggctgg agaatgaggc cgagaaaccg agcaatgcgt tgttaactag agcttggtct
     6841 ccaggatgga gcaatgctga taagttacta aatgagttca tcgagaagca gttgatagat
     6901 tatgcaaaga acagcaagaa agttgttggg aattctactt cactactttc tccgtatctc
     6961 catttcgggg aaataagcgt cagacacgtt ttccagtgtg cccggatgaa acaaattata
     7021 tgggcaagag ataagaacag tgaaggagaa gaaagtgcag atctttttct taggggaatc
     7081 ggtttaagag agtattctcg gtatatatgt ttcaacttcc cgtttactca cgagcaatcg
     7141 ttgttgagtc atcttcggtt tttcccttgg gatgctgatg ttgataagtt caaggcctgg
     7201 agacaaggca ggaccggtta tccgttggtg gatgccggaa tgagagagct ttgggctacc
     7261 ggatggatgc ataacagaat aagagtgatt gtttcaagct ttgctgtgaa gtttcttctc
     7321 cttccatgga aatggggaat gaagtatttc tgggatacac ttttggatgc tgatttggaa
     7381 tgtgacatcc ttggctggca gtatatctct gggagtatcc ccgatggcca cgagcttgat
     7441 cgcttggaca atcccgcgtt acaaggcgcc aaatatgacc cagaaggtga gtacataagg
     7501 caatggcttc ccgagcttgc gagattgcca actgaatgga tccatcatcc atgggacgct
     7561 cctttaaccg tactcaaagc ttctggtgtg gaactcggaa caaactatgc gaaacccatt
     7621 gtagacatcg acacagctcg tgagctacta gctaaagcta tttcaagaac ccgtgaagca
     7681 cagatcatga tcggagcagc agcccgggat ccaccggtcg ccacctagcc tgcaggcggc
     7741 cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga gtcgacctgc
     7801 aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg
     7861 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     7921 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     7981 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     8041 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     8101 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     8161 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     8221 cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg
     8281 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     8341 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     8401 ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa ttcgagctcg
     8461 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8521 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8581 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8641 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8701 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8761 agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga
     8821 tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac aagcctaatt
     8881 gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact gccgtcagag
     8941 tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg
     9001 tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg
     9061 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     9121 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     9181 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     9241 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     9301 agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     9361 cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
     9421 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
     9481 tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat
     9541 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
     9601 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     9661 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     9721 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     9781 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     9841 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     9901 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     9961 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
    10021 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
    10081 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
    10141 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
    10201 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
    10261 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
    10321 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
    10381 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
    10441 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
    10501 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
    10561 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
    10621 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
    10681 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    10741 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    10801 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    10861 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
    10921 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    10981 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    11041 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    11101 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    11161 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    11221 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    11281 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    11341 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    11401 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    11461 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
    11521 cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt cagttagggt
    11581 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt
    11641 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc
    11701 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc
    11761 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
    11821 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
    11881 taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt gagaatacca
    11941 ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg tgaaatactg
    12001 gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc gaacactttt
    12061 taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat cgtcacctgg
    12121 gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc tgaacaatgg
    12181 aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac
    12241 tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac aaccagcgcg
    12301 agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt attgatgacc
    12361 tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg
    12421 tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt gttgatatcc
    12481 cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc ccgccaatct
    12541 ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt aacttcaggc
    12601 gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca ggcaaacctg
    12661 agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct agtccgccgc
    12721 tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac catccctcac
    12781 tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc
    12841 ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga tgatttatac
    12901 gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc ggatctttgt
    12961 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
    13021 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
    13081 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
    13141 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
    13201 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
    13261 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
    13321 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
    13381 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
    13441 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
    13501 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
    13561 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
    13621 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
    13681 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    13741 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    13801 tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca taccctatta
    13861 ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    13921 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt ggaagggcta
    13981 attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac acaaggctac
    14041 ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact gacctttgga
    14101 tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa taaaggagag
    14161 aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga gagagaagtg
    14221 ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga gctgcatccg
    14281 gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc tggggacttt
    14341 ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc ctgcatataa
    14401 gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
    14461 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
    14521 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
    14581 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
    14641 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
    14701 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
    14761 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
    14821 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
    14881 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
    14941 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
    15001 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
    15061 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccggc
    15121 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
    15181 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
    15241 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
    15301 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
    15361 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
    15421 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg g
//
