LOCUS       sequence-336517-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express 3xFLAG-Kctd19 under the mouse Clgn promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Oura S, Koyano T, Kodera C, Horisawa-Takada Y, Matsuyama M, Ishiguro
            KI, Ikawa M
  TITLE     KCTD19 and its associated protein ZFP541 are independently essential
            for meiosis in male mice.
  JOURNAL   PLoS Genet. 2021 May 7;17(5):e1009412. doi: 
            10.1371/journal.pgen.1009412. eCollection 2021 May.
  PUBMED    33961623
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2021-05-7"; volume: "17(5):e1009412. doi"; pages: " 
            10.1371/journal.pgen.1009412. eCollection 2021 May"
COMMENT     Sequence Label: pClgn1.1_3xFLAG-Kctd19
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      155..164
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             164..229
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atcgctctgg ggagacgccg ggcaccaagt agcggccagt cggacacctg agctgccgct
       61 gctggacaca agcccgcagg acaggtgtgt agaccgcggg ccgccttcct gcggcttgtt
      121 ctctggggac cgggtgagaa atcggatctc tagagccgcc atggactaca aagaccatga
      181 cggtgattat aaagatcatg acattgatta caaggatgac gatgacaagg ggggcggggc
      241 tagcggatct gaggagcctg gcgggctcca tgagtcagca gaggatttgt ttcacttcaa
      301 tgtcgggggc tggcatttct cagttcccag gagcaaactt gctcagttcc cagactcgct
      361 gttgtggaaa gaggcttcag ccctgacctc ttcagagaac cagcggctct tcattgacag
      421 agacggctcc acgtttaggc atgtgcacta ttacctctac acgtccaagc tctccttctc
      481 cagttgtgca gaactgaact tgctctatga gcaagccctg ggtttgcagc tgatgccctt
      541 gcttcagacc ctagataacc tgaaggaagg gagacaccac ctccgtgtgc ggcctgccga
      601 cattcctgtg gctgagagag catccctgaa ctactggcgg acatggaagt gtatcagcaa
      661 gccctcggat tttccaataa aaagccccgc cttcacaggt cttcatgaca aggccccttt
      721 gggactcatg gacacaccgc tgttggacac agaagaggaa gtgcactact gcttcttacc
      781 cctcgacttg gtggccaagt acccaagcct agtgaccgaa gacaatctgc tgtggctggc
      841 tgaaacggtg gccctgatcg agtgcgaatg cagcgagttc cgcttcattg tgaa
//
