LOCUS       sequence-33652-e         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-V2
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(21..427)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    494..527
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(569..585)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(570..586)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    644..761
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gctcaccatg gtggcgggat ccgggcgact cagtcaatcg gaggactggc gcgccgagtg
       61 aggggttgtg agctctttta tagagctcgg gaagcagaag cgcgcgaaca gaagcgagaa
      121 gcaggctgat tggttaattc aaataaggcg cagggtcatt tcaggtcctt gggggagcct
      181 ggaaacatct gatgggtctt aagaaactgc tgagggttgg gccatatctg gggaccatct
      241 gttcttggcc tcgggccggg gccgaaactg cggtgaccat ctgttcttgg ccccgggccg
      301 gggccgaaac tgctcaccgc agatatcctg tttggcccaa cgttagctat tttcatgtac
      361 ccgcccttga tctgaacttc tctattcttg gtttggtatt tttccatgcc ttgcaaaatg
      421 gcgttactgc agctagcttg ccaaacctac aggtggggtc tttcagcggc cgcttaagct
      481 tggaaccctt aatataactt cgtataatgt atgctatacg aagttattag gtccctcgac
      541 ctgctggaat ctcgtgaagc gagcttatcg ataccgtcga cctcgactag agcgggcccg
      601 gtactaacca aactggatct ctgctgtccc tgtaataaac ccgaaaattt tgaatttttg
      661 taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct actattcttt
      721 cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg aatactgcca
      781 tttgtctgca gaattggcgc acgcagtgcc gatccgttca ctaatcgaat ggatctgtct
      841 ctgtctctct ct
//
