LOCUS       sequence-33654-e         600 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 600)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iG2
FEATURES             Location/Qualifiers
     source          1..600
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    27..579
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    29..596
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(189..208)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     418..435
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gcccttgctc accatggttg tggccatatt atcatcgtgt ttttcaaagg aaaaccacgt
       61 ccccgtggtt cggggggcct agacgttttt ttaacctcga ctaaacacat gtaaagcatg
      121 tgcaccgagg ccccagatca gatcccatac aatggggtac cttctgggca tccttcagcc
      181 ccttgttgaa tacgcttgag gagagccatt tgactctttc cacaactatc caactcacaa
      241 cgtggcactg gggttgtgcc gcctttgcag gtgtatctta tacacgtggc ttttggccgc
      301 agaggcacct gtcgccaggt ggggggttcc gctgcctgca aagggtcgct acagacgttg
      361 tttgtcttca agaagcttcc agaggaactg cttccttcac gacattcaac agaccttgca
      421 ttcctttggc gagaggggaa agacccctag gaatgctcgt caagaagaca gggccaggtt
      481 tccgggccct cacattgcca aaagacggca atatggtgga aaataacata tagacaaacg
      541 cacaccggcc ttattccaag cggcttcggc cagtaacgtt aggggggggg gagggagagg
//
