LOCUS       sequence-33655-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-T2
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      32..41
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     439..458
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     633..656
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtcctccgat tgactgagtc gcccggatcc cgccaccatg gtgagcaagg gcgaggaggt
       61 catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccatgaacg gccacgagtt
      121 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
      181 ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc agttcatgta
      241 cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga agctgtcctt
      301 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc tggtgaccgt
      361 gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga tgcgcggcac
      421 caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctccac
      481 cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg ccctgaagct
      541 gaaggacggc ggccactacc tggtggagtt caagaccatc tacatggcca agaagcccgt
      601 gcaactgccc ggctactact acgtggacac caagctggac atcacctccc acaacgagga
      661 ctacaccatc gtggaacagt acgagcgctc cgagggccgc caccacctgt tcctggggca
      721 tggcaccggc agcaccggca gcggcagctc cggcaccgcc tcctccgagg acaacaacat
      781 ggccgtcatc aaagagttca tgcgcttcaa ggtgcgcatg gagggctcca tgaacggcca
      841 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      901 gctgaagg
//
