LOCUS       sequence-33661-e         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-G
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      14..23
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(29..435)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    502..535
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(577..593)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(578..594)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(671..691)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tcgcccttgc tcaccatggt ggcgggatcc gggcgactca gtcaatcgga ggactggcgc
       61 gccgagtgag gggttgtgag ctcttttata gagctcggga agcagaagcg cgcgaacaga
      121 agcgagaagc aggctgattg gttaattcaa ataaggcgca gggtcatttc aggtccttgg
      181 gggagcctgg aaacatctga tgggtcttaa gaaactgctg agggttgggc catatctggg
      241 gaccatctgt tcttggcctc gggccggggc cgaaactgcg gtgaccatct gttcttggcc
      301 ccgggccggg gccgaaactg ctcaccgcag atatcctgtt tggcccaacg ttagctattt
      361 tcatgtaccc gcccttgatc tgaacttctc tattcttggt ttggtatttt tccatgcctt
      421 gcaaaatggc gttactgcag ctagcttgcc aaacctacag gtggggtctt tcagcggccg
      481 cttaagcttg gaacccttaa tataacttcg tataatgtat gctatacgaa gttattaggt
      541 ccctcgacct gctggaatct cgtgaagcga gcttatcgat accgtcgacc tcgagggtca
      601 ccgcgcgtta acaaggcttt tctccaaggg atatttatag tctcaaaaca cacaattact
      661 ttacagttag ggtgagtttc cttttgtgct gttttttaaa ataataattt agtatttgta
      721 tctcttatag aaatccaagc ctatcatgta aaatgtagct agtattaaaa agaacagatt
      781 atctgtcttt tatcgcacat taagcctcta tagttactag gaaatattat atgcaaatta
      841 accggggcag gggagtagcc gagcttctcc cacaagtctg tgcgaggggg ccggcgcggg
      901 cctagagatg gc
//
