LOCUS       sequence-33662-e         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-C
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      112..121
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(127..533)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    600..633
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(675..691)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(676..692)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(769..789)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cgccctcgcc ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga
       61 agcgcatgaa ctccttgatg atggccatgt tatcctcctc gcccttgctc accatggtgg
      121 cgggatccgg gcgactcagt caatcggagg actggcgcgc cgagtgaggg gttgtgagct
      181 cttttataga gctcgggaag cagaagcgcg cgaacagaag cgagaagcag gctgattggt
      241 taattcaaat aaggcgcagg gtcatttcag gtccttgggg gagcctggaa acatctgatg
      301 ggtcttaaga aactgctgag ggttgggcca tatctgggga ccatctgttc ttggcctcgg
      361 gccggggccg aaactgcggt gaccatctgt tcttggcccc gggccggggc cgaaactgct
      421 caccgcagat atcctgtttg gcccaacgtt agctattttc atgtacccgc ccttgatctg
      481 aacttctcta ttcttggttt ggtatttttc catgccttgc aaaatggcgt tactgcagct
      541 agcttgccaa acctacaggt ggggtctttc agcggccgct taagcttgga acccttaata
      601 taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgacctgc tggaatctcg
      661 tgaagcgagc ttatcgatac cgtcgacctc gagggtcacc gcgcgttaac aaggcttttc
      721 tccaagggat atttatagtc tcaaaacaca caattacttt acagttaggg tgagtttcct
      781 tttgtgctgt tttttaaaat aataatttag tatttgtatc tcttatagaa atccaagcct
      841 atcatgtaaa atgtagctag tattaaaaag aacagattat ctgtctttta tcgcacatta
      901 agcctctata gttacta
//
