LOCUS       sequence-33663-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-T
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        91..403
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     322..342
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     419..435
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     420..436
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(478..511)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
       61 cagtttggtt agtaccgggc ccgctctaga gatccgacgc gccatctcta ggcccgcgcc
      121 ggccccctcg cacagacttg tgggagaagc tcggctactc ccctgccccg gttaatttgc
      181 atataatatt tcctagtaac tatagaggct taatgtgcga taaaagacag ataatctgtt
      241 ctttttaata ctagctacat tttacatgat aggcttggat ttctataaga gatacaaata
      301 ctaaattatt attttaaaaa acagcacaaa aggaaactca ccctaactgt aaagtaattg
      361 tgtgttttga gactataaat atcccttgga gaaaagcctt gttaacgcgc ggtgaccctc
      421 gaggtcgacg gtatcgataa gctcgcttca cgagattcca gcaggtcgag ggacctaata
      481 acttcgtata gcatacatta tacgaagtta tattaagggt tccaagctta agcggccgct
      541 gaaagacccc acctgtaggt ttggcaagct agctgcagta acgccatttt gcaaggcatg
      601 gaaaaatacc aaaccaagaa tagagaagtt cagatcaagg gcgggtacat gaaaatagct
      661 aacgttgggc caaacaggat atctgcggtg agcagtttcg gccccggccc ggggccaaga
      721 acagatggtc accgcagttt cggccccggc ccgaggccaa gaacagatgg tccccagata
      781 tggcccaacc ctcagcagtt tcttaagacc catcagatgt ttccaggctc ccccaaggac
      841 ctgaaatgac cctgcgcctt atttgaatta accaatcagc ctgcttctcg cttctgttcg
      901 cgcgcttctg cttcccgagc tctataaa
//
