LOCUS       sequence-33666-e         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-Cer
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(6..412)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    479..512
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(554..570)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(555..571)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(648..668)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gggatccggg cgactcagtc aatcggagga ctggcgcgcc gagtgagggg ttgtgagctc
       61 ttttatagag ctcgggaagc agaagcgcgc gaacagaagc gagaagcagg ctgattggtt
      121 aattcaaata aggcgcaggg tcatttcagg tccttggggg agcctggaaa catctgatgg
      181 gtcttaagaa actgctgagg gttgggccat atctggggac catctgttct tggcctcggg
      241 ccggggccga aactgcggtg accatctgtt cttggccccg ggccggggcc gaaactgctc
      301 accgcagata tcctgtttgg cccaacgtta gctattttca tgtacccgcc cttgatctga
      361 acttctctat tcttggtttg gtatttttcc atgccttgca aaatggcgtt actgcagcta
      421 gcttgccaaa cctacaggtg gggtctttca gcggccgctt aagcttggaa cccttaatat
      481 aacttcgtat aatgtatgct atacgaagtt attaggtccc tcgacctgct ggaatctcgt
      541 gaagcgagct tatcgatacc gtcgacctcg agggtcaccg cgcgttaaca aggcttttct
      601 ccaagggata tttatagtct caaaacacac aattacttta cagttagggt gagtttcctt
      661 ttgtgctgtt ttttaaaata ataatttagt atttgtatct cttatagaaa tccaagccta
      721 tcatgtaaaa tgtagctagt attaaaaaga acagattatc tgtcttttat cgcacattaa
      781 gcctctatag ttactaggaa atattatatg caaattaacc ggggcagggg agtagccgag
      841 cttctcccac aagtctgtgc
//
