LOCUS       sequence-33667-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-1xT
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      95..104
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(110..516)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    583..616
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(658..674)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(659..675)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(752..772)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 tcgccctcga tctcgaactc gtggccgttc atggagccct ccatgcgcac cttgaagcgc
       61 atgaactctt tgatgacctc ctcgcccttg ctcaccatgg tggcgggatc cgggcgactc
      121 agtcaatcgg aggactggcg cgccgagtga ggggttgtga gctcttttat agagctcggg
      181 aagcagaagc gcgcgaacag aagcgagaag caggctgatt ggttaattca aataaggcgc
      241 agggtcattt caggtccttg ggggagcctg gaaacatctg atgggtctta agaaactgct
      301 gagggttggg ccatatctgg ggaccatctg ttcttggcct cgggccgggg ccgaaactgc
      361 ggtgaccatc tgttcttggc cccgggccgg ggccgaaact gctcaccgca gatatcctgt
      421 ttggcccaac gttagctatt ttcatgtacc cgcccttgat ctgaacttct ctattcttgg
      481 tttggtattt ttccatgcct tgcaaaatgg cgttactgca gctagcttgc caaacctaca
      541 ggtggggtct ttcagcggcc gcttaagctt ggaaccctta atataacttc gtataatgta
      601 tgctatacga agttattagg tccctcgacc tgctggaatc tcgtgaagcg agcttatcga
      661 taccgtcgac ctcgagggtc accgcgcgtt aacaaggctt ttctccaagg gatatttata
      721 gtctcaaaac acacaattac tttacagtta gggtgagttt ccttttgtgc tgttttttaa
      781 aataataatt tagtatttgt atctcttata gaaatccaag cctatcatgt aaaatgtagc
      841 tagtattaaa aagaacagat tatctgtc
//
