LOCUS       sequence-33668-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-1xT/BSD
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..457
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(488..521)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(630..650)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 gtacgagcgc tccgagggcc gccaccacct gttcctgtac ggcatggacg agctgtacaa
       61 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
      121 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
      181 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
      241 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
      301 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
      361 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
      421 tgaattgctg ccctctggtt atgtgtggga gggctaatgt acaagtaaga attcgtcgag
      481 ggacctaata acttcgtata gcatacatta tacgaagtta tacatgttta agggttccgg
      541 ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga ttacaaaatt
      601 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
      661 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
      721 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
      781 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
      841 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
      901 gcctgccttg cccgctgctg gacagggctc ggctgtg
//
