LOCUS       sequence-33669-e         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-1xT/BSD
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      93..102
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(108..514)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    581..614
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(656..672)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(657..673)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(750..770)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gccctcgatc tcgaactcgt ggccgttcat ggagccctcc atgcgcacct tgaagcgcat
       61 gaactctttg atgacctcct cgcccttgct caccatggtg gcgggatccg ggcgactcag
      121 tcaatcggag gactggcgcg ccgagtgagg ggttgtgagc tcttttatag agctcgggaa
      181 gcagaagcgc gcgaacagaa gcgagaagca ggctgattgg ttaattcaaa taaggcgcag
      241 ggtcatttca ggtccttggg ggagcctgga aacatctgat gggtcttaag aaactgctga
      301 gggttgggcc atatctgggg accatctgtt cttggcctcg ggccggggcc gaaactgcgg
      361 tgaccatctg ttcttggccc cgggccgggg ccgaaactgc tcaccgcaga tatcctgttt
      421 ggcccaacgt tagctatttt catgtacccg cccttgatct gaacttctct attcttggtt
      481 tggtattttt ccatgccttg caaaatggcg ttactgcagc tagcttgcca aacctacagg
      541 tggggtcttt cagcggccgc ttaagcttgg aacccttaat ataacttcgt ataatgtatg
      601 ctatacgaag ttattaggtc cctcgacctg ctggaatctc gtgaagcgag cttatcgata
      661 ccgtcgacct cgagggtcac cgcgcgttaa caaggctttt ctccaaggga tatttatagt
      721 ctcaaaacac acaattactt tacagttagg gtgagtttcc ttttgtgctg ttttttaaaa
      781 taataattta gtatttgtat ctcttataga aatccaagcc tatcatgtaa aatgtagcta
      841 gtattaaaaa gaacagatta tctgtctttt atcgcacatt aagcctctat agttactagg
      901 aaatattata tgc
//
