LOCUS       sequence-33670-e         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-G/BSD
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..419
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="AKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTGV
                     NVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGIK
                     AIVKDSDGQPTAVGIRELLPSGYVWEG"
     protein_bind    complement(450..483)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(592..612)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 tcggcatgga cgagctgtac aaggccaagc ctttgtctca agaagaatcc accctcattg
       61 aaagagcaac ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg
      121 cagctctctc tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg
      181 gaccttgtgc agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga
      241 cttgtatcgt cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc
      301 gacaggtgct tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac
      361 agccgacggc agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaat
      421 gtacaagtaa gaattcgtcg agggacctaa taacttcgta tagcatacat tatacgaagt
      481 tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc ttatcgataa
      541 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
      601 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
      661 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
      721 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
      781 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
      841 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacaggg
//
