LOCUS       sequence-33671-e         832 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 832)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-G/BSD
FEATURES             Location/Qualifiers
     source          1..832
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      12..21
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(27..433)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    500..533
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(575..591)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(576..592)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(669..689)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 gcccttgctc accatggtgg cgggatccgg gcgactcagt caatcggagg actggcgcgc
       61 cgagtgaggg gttgtgagct cttttataga gctcgggaag cagaagcgcg cgaacagaag
      121 cgagaagcag gctgattggt taattcaaat aaggcgcagg gtcatttcag gtccttgggg
      181 gagcctggaa acatctgatg ggtcttaaga aactgctgag ggttgggcca tatctgggga
      241 ccatctgttc ttggcctcgg gccggggccg aaactgcggt gaccatctgt tcttggcccc
      301 gggccggggc cgaaactgct caccgcagat atcctgtttg gcccaacgtt agctattttc
      361 atgtacccgc ccttgatctg aacttctcta ttcttggttt ggtatttttc catgccttgc
      421 aaaatggcgt tactgcagct agcttgccaa acctacaggt ggggtctttc agcggccgct
      481 taagcttgga acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc
      541 ctcgacctgc tggaatctcg tgaagcgagc ttatcgatac cgtcgacctc gagggtcacc
      601 gcgcgttaac aaggcttttc tccaagggat atttatagtc tcaaaacaca caattacttt
      661 acagttaggg tgagtttcct tttgtgctgt tttttaaaat aataatttag tatttgtatc
      721 tcttatagaa atccaagcct atcatgtaaa atgtagctag tattaaaaag aacagattat
      781 ctgtctttta tcgcacatta agcctctata gttactagga aatattatat gc
//
