LOCUS       sequence-33672-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-R
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      146..155
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(161..567)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    634..667
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(709..725)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(710..726)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(803..823)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ttggtcacct tcagcttcac ggtgttgtgg ccctcgtagg ggcggccctc gccctcgccc
       61 tcgatctcga actcgtggcc gttcacggtg ccctccatgc gcaccttgaa gcgcatgaac
      121 tcggtgatga cgttctcgga ggaggccatg gtggcgggat ccgggcgact cagtcaatcg
      181 gaggactggc gcgccgagtg aggggttgtg agctctttta tagagctcgg gaagcagaag
      241 cgcgcgaaca gaagcgagaa gcaggctgat tggttaattc aaataaggcg cagggtcatt
      301 tcaggtcctt gggggagcct ggaaacatct gatgggtctt aagaaactgc tgagggttgg
      361 gccatatctg gggaccatct gttcttggcc tcgggccggg gccgaaactg cggtgaccat
      421 ctgttcttgg ccccgggccg gggccgaaac tgctcaccgc agatatcctg tttggcccaa
      481 cgttagctat tttcatgtac ccgcccttga tctgaacttc tctattcttg gtttggtatt
      541 tttccatgcc ttgcaaaatg gcgttactgc agctagcttg ccaaacctac aggtggggtc
      601 tttcagcggc cgcttaagct tggaaccctt aatataactt cgtataatgt atgctatacg
      661 aagttattag gtccctcgac ctgctggaat ctcgtgaagc gagcttatcg ataccgtcga
      721 cctcgagggt caccgcgcgt taacaaggct tttctccaag ggatatttat agtctcaaaa
      781 cacacaatta ctttacagtt agggtgagtt tccttttgtg ctgtttttta aaataataat
      841 ttagtatttg tatctc
//
