LOCUS       sequence-33673-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-X
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      148..157
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(163..569)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    636..669
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(711..727)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(712..728)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(805..825)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 ccttggtcac cttcagcttg gcggtctggg tgccctcgta ggggcggccc tcgccctcgc
       61 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gcgcaccttg aagcgcatga
      121 actccttgat gacgtcctcg gaggaggcca tggtggcggg atccgggcga ctcagtcaat
      181 cggaggactg gcgcgccgag tgaggggttg tgagctcttt tatagagctc gggaagcaga
      241 agcgcgcgaa cagaagcgag aagcaggctg attggttaat tcaaataagg cgcagggtca
      301 tttcaggtcc ttgggggagc ctggaaacat ctgatgggtc ttaagaaact gctgagggtt
      361 gggccatatc tggggaccat ctgttcttgg cctcgggccg gggccgaaac tgcggtgacc
      421 atctgttctt ggccccgggc cggggccgaa actgctcacc gcagatatcc tgtttggccc
      481 aacgttagct attttcatgt acccgccctt gatctgaact tctctattct tggtttggta
      541 tttttccatg ccttgcaaaa tggcgttact gcagctagct tgccaaacct acaggtgggg
      601 tctttcagcg gccgcttaag cttggaaccc ttaatataac ttcgtataat gtatgctata
      661 cgaagttatt aggtccctcg acctgctgga atctcgtgaa gcgagcttat cgataccgtc
      721 gacctcgagg gtcaccgcgc gttaacaagg cttttctcca agggatattt atagtctcaa
      781 aacacacaat tactttacag ttagggtgag tttccttttg tgctgttttt taaaataata
      841 atttagtatt tgtatctctt atagaaatcc aagcctatca tgtaaaatgt agctagta
//
