LOCUS       sequence-33677-e         818 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 818)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 818)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-E
FEATURES             Location/Qualifiers
     source          1..818
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      13..22
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(28..434)
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     protein_bind    501..534
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(576..592)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(577..593)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(670..690)
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
ORIGIN
        1 cgcccttgct caccatggtg gcgggatccg ggcgactcag tcaatcggag gactggcgcg
       61 ccgagtgagg ggttgtgagc tcttttatag agctcgggaa gcagaagcgc gcgaacagaa
      121 gcgagaagca ggctgattgg ttaattcaaa taaggcgcag ggtcatttca ggtccttggg
      181 ggagcctgga aacatctgat gggtcttaag aaactgctga gggttgggcc atatctgggg
      241 accatctgtt cttggcctcg ggccggggcc gaaactgcgg tgaccatctg ttcttggccc
      301 cgggccgggg ccgaaactgc tcaccgcaga tatcctgttt ggcccaacgt tagctatttt
      361 catgtacccg cccttgatct gaacttctct attcttggtt tggtattttt ccatgccttg
      421 caaaatggcg ttactgcagc tagcttgcca aacctacagg tggggtcttt cagcggccgc
      481 ttaagcttgg aacccttaat ataacttcgt ataatgtatg ctatacgaag ttattaggtc
      541 cctcgacctg ctggaatctc gtgaagcgag cttatcgata ccgtcgacct cgagggtcac
      601 cgcgcgttaa caaggctttt ctccaaggga tatttatagt ctcaaaacac acaattactt
      661 tacagttagg gtgagtttcc ttttgtgctg ttttttaaaa taataattta gtatttgtat
      721 ctcttataga aatccaagcc tatcatgtaa aatgtagcta gtattaaaaa gaacagatta
      781 tctgtctttt atcgcacatt aagcctctat agttacta
//
