LOCUS       sequence-33678-e         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iV
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        105..417
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     336..356
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     433..449
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     434..450
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(492..525)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
       61 ggacagcaga gatccagttt ggttagtacc gggcccgctc tagagatccg acgcgccatc
      121 tctaggcccg cgccggcccc ctcgcacaga cttgtgggag aagctcggct actcccctgc
      181 cccggttaat ttgcatataa tatttcctag taactataga ggcttaatgt gcgataaaag
      241 acagataatc tgttcttttt aatactagct acattttaca tgataggctt ggatttctat
      301 aagagataca aatactaaat tattatttta aaaaacagca caaaaggaaa ctcaccctaa
      361 ctgtaaagta attgtgtgtt ttgagactat aaatatccct tggagaaaag ccttgttaac
      421 gcgcggtgac cctcgaggtc gacggtatcg ataagctcgc ttcacgagat tccagcaggt
      481 cgagggacct aataacttcg tatagcatac attatacgaa gttatattaa gggttccaag
      541 cttaagcggc cggccgctga aagaccccac ctgtaggttt ggcaagctag ctgcagtaac
      601 gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca gatcaagggc
      661 gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag cagtttcggc
      721 cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc gaggccaaga
      781 acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca tcagatgttt
      841 ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac caatcagcct
      901 gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tat
//
