LOCUS       sequence-336787-        8539 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega1 Basic cloning vector GB2.0 compatible P[acman]-derived 
            backbone. Allows for copy induction from low to high using AutoFOS 
            or similar. Requires EPI300 bacterial strain (DH10B Derived) or 
            similar. uses blue-white screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8539)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 8539)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: P[acman]-Omega1
FEATURES             Location/Qualifiers
     source          1..8539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    join(428..458,459..1013)
                     /label=P element 5' end
     primer_bind     1051..1070
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1264..1286
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1278..1295
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1279..1295
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1302..1321
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1302..1320
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1323..1379
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     promoter        complement(1386..1404)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1387..1404)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     promoter        complement(1458..1505)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     misc_feature    join(1537..1738,1739..1769)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    1984..2053
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             complement(2193..2852)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2767..2786
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(2853..2955)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     rep_origin      3890..4525
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4612..4831
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     CDS             4922..5677
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     misc_feature    5680..5930
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             6256..7431
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     CDS             7431..8402
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
ORIGIN
        1 accacggtcc cactcgtatc gtcggtctga ttattagtct gggaccacgg tcccactcgt
       61 atcgtcggtc tgattattag tctgggacca cgatcccact cgtgttgtcg gtctgattat
      121 cggtctggga ccacggtccc acttgtattg tcgatcagac tatcagcgtg agactacgat
      181 tccatcaatg cctgtcaagg gcaagtattg acatgtcgtc gtaacctgta gaacggagta
      241 acctcggtgt gcggttgtat gcctgctgtg gattgctgct gtgtcctgct tatccacaac
      301 attttgcgca cggttatgtg gacaaaatac ctggttaccc aggccgtgcc ggcacgttaa
      361 ccgggctgca tccgatgcaa gtgtgtcgct gtcgagatcc actagtctgg actacgtggg
      421 tctggcccat gatgaaataa cataaggtgg tcccgtcgat agccgaagct taccgaagta
      481 tacacttaaa ttcagtgcac gtttgcttgt tgagaggaaa ggttgtgtgc ggacgaattt
      541 ttttttgaaa acattaaccc ttacgtggaa taaaaaaaaa tgaaatattg caaattttgc
      601 tgcaaagctg tgactggagt aaaattaatt cacgtgccga agtgtgctat taagagaaaa
      661 ttgtgggagc agagccttgg gtgcagcctt ggtgaaaact cccaaatttg tgatacccac
      721 tttaatgatt cgcagtggaa ggctgcacct gcaaaaggtc agacatttaa aaggaggcga
      781 ctcaacgcag atgccgtacc tagtaaagtg atagagcctg aaccagaaaa gataaaagaa
      841 ggctatacca gtgggagtac acaaacagag taagtttgaa tagtaaaaaa aatcatttat
      901 gtaaacaata acgtgactgt gcgttaggtc ctgttcattg tttaatgaaa ataagagctt
      961 gagggaaaaa attcgtactt tggagtacga aatgcgtcgt ttagagcagc agcgtcagga
     1021 tccggtctca tgacagcgag agacgctatg cggcatcaga gcagattgta ctgagagtgc
     1081 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgtc
     1141 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     1201 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
     1261 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg
     1321 gcgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca tgcaagcttg
     1381 agtattctat agtgtcacct aaatagcttg gcgtaatcat ggtcatgatc tgcctcctgg
     1441 gtttagttcc tcaccttgtc gtattatact atgccgatat actatgccga tgattaattg
     1501 tcaacaggcg tctctctcct gagaccggat ccgttatgcg tactcgcaaa ttattaaaaa
     1561 taaaacttta aaaataattt cgtctaatta atattatgag ttaattcaaa ccccacggac
     1621 atgctaaggg ttaatcaaca atcatatcgc tgtctcactc agactcaata cgacactcag
     1681 aatactattc ctttcactcg cacttattgc aagcatacgt taagtggatg tctcttgccg
     1741 acgggaccac cttatgttat ttcatcatgg tctggccatt ctcatcgtga tcgacatgcc
     1801 cgccgtgacc gtcgagaacc cgctgacgct gccccgcgta tccgcacccg ccgacgccgt
     1861 cgcacgtccc gtgctcaccg tgaccaccgc gcccagcggt ttcgagggcg agggcttccc
     1921 ggtgcgccgc gcgttcgccg ggatcaacta ccgccacctc gacccgttca tcatgatgga
     1981 ccagatgggt gaggtggagt acgcgcccgg ggagcccaag ggcacgccct ggcacccgca
     2041 ccgcggcttc gagacagtga cctacatcgt cgaccaattc aacatcatgt ttgacagctt
     2101 atcatcgaat ttctgccatt catccgctta ttatcactta ttcaggcgta gcaaccaggc
     2161 gtttaagggc accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag
     2221 tactgttgta attcattaag cattctgccg acatggaagc catcacaaac ggcatgatga
     2281 acctgaatcg ccagcggcat cagcaccttg tcgccttgcg tataatattt gcccatggtg
     2341 aaaacggggg cgaagaagtt gtccatattg gccacgttta aatcaaaact ggtgaaactc
     2401 acccagggat tggctgacac gaaaaacata ttctcaataa accctttagg gaaataggcc
     2461 aggttttcac cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg
     2521 tcgtggtatt cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa
     2581 caagggtgaa cactatccca tatcaccagc tcaccgtctt tcattgccat acggaattcc
     2641 ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta
     2701 tttttcttta cggtctttaa aaaggccgta atatccagct gaacggtctg gttataggta
     2761 cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca
     2821 acggtggtat atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc
     2881 gataactcaa aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct
     2941 cttacgtgcc gatcatcact ctcattttcg ccaaaagttg gcccagggct tcccggtatc
     3001 aacagggaca ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc
     3061 gcgataagct catggagcgg cgtaaccgtc gcacaggaag gacagagaaa gcgcggatct
     3121 gggaagtgac ggacagaacg gtcaggacct ggattgggga ggcggttgcc gccgctgctg
     3181 ctgacggtgt gacgttctct gttccggtca caccacatac gttccgccat tcctatgcga
     3241 tgcacatgct gtatgccggt ataccgctga aagttctgca aagcctgatg ggacataagt
     3301 ccatcagttc aacggaagtc tacacgaagg tttttgcgct ggatgtggct gcccggcacc
     3361 gggtgcagtt tgcgatgccg gagtctgatg cggttgcgat gctgaaacaa ttatcctgag
     3421 aataaatgcc ttggccttta tatggaaatg tggaactgag tggatatgct gtttttgtct
     3481 gttaaacaga gaagctggct gttatccact gagaagcgaa cgaaacagtc gggaaaatct
     3541 cccattatcg tagagatccg cattattaat ctcaggagcc tgtgtagcgt ttataggaag
     3601 tagtgttctg tcatgatgcc tgcaagcggt aacgaaaacg atttgaatat gccttcagga
     3661 acaatagaaa tcttcgtgcg gtgttacgtt gaagtggagc ggattatgtc agcaatggac
     3721 agaacaacct aatgaacaca gaaccatgat gtggtctgtc cttttacagc cagtagtgct
     3781 cgccgcagtc gagcgacagg gcgaagccct cgagatcccc tgccattttt ggggtgaggc
     3841 cgttcgcggc cgaggggcgc agcccctggg gggatgggag gcccgcgtta gcgggccggg
     3901 agggttcgag aagggggggc accccccttc ggcgtgcgcg gtcacgcgca cagggcgcag
     3961 ccctggttaa aaacaaggtt tataaatatt ggtttaaaag caggttaaaa gacaggttag
     4021 cggtggccga aaaacgggcg gaaacccttg caaatgctgg attttctgcc tgtggacagc
     4081 ccctcaaatg tcaataggtg cgcccctcat ctgtcagcac tctgcccctc aagtgtcaag
     4141 gatcgcgccc ctcatctgtc agtagtcgcg cccctcaagt gtcaataccg cagggcactt
     4201 atccccaggc ttgtccacat catctgtggg aaactcgcgt aaaatcaggc gttttcgcag
     4261 atttgcgagg ctggccagct ccacgtcgcc ggccgaaatc gagcctgccc ctcatctgtc
     4321 aacgccgcgc cgggtgagtc ggcccctcaa gtgtcaacgt ccgcccctca tctgtcagtg
     4381 agggccaagt tttccgcgag gtatccacaa cgccggcggc cggccgcggt gtctcgcaca
     4441 cggcttcgac ggcgtttctg gcgcgtttgc agggccatag acggccgcca gcccagcggc
     4501 gagggcaacc agcccggtga gcgtcggaaa ggggaatttc gagtgagcga ggaagcacca
     4561 gggaacagca cttatatatt ctgcttacac acgatgcctg aaaaaacttc ccttggggtt
     4621 atccacttat ccacggggat atttttataa ttattttttt tatagttttt agatcttctt
     4681 ttttagagcg ccttgtaggc ctttatccat gctggttcta gagaaggtgt tgtgacaaat
     4741 tgccctttca gtgtgacaaa tcaccctcaa atgacagtcc tgtctgtgac aaattgccct
     4801 taaccctgtg acaaattgcc ctcagaagaa gctgtttttt cacaaagtta tccctgctta
     4861 ttgactcttt tttatttagt gtgacaatct aaaaacttgt cacacttcac atggatctgt
     4921 catggcggaa acagcggtta tcaatcacaa gaaacgtaaa aatagcccgc gaatcgtcca
     4981 gtcaaacgac ctcactgagg cggcatatag tctctcccgg gatcaaaaac gtatgctgta
     5041 tctgttcgtt gaccagatca gaaaatctga tggcacccta caggaacatg acggtatctg
     5101 cgagatccat gttgctaaat atgctgaaat attcggattg acctctgcgg aagccagtaa
     5161 ggatatacgg caggcattga agagtttcgc ggggaaggaa gtggtttttt atcgccctga
     5221 agaggatgcc ggcgatgaaa aaggctatga atcttttcct tggtttatca aacgtgcgca
     5281 cagtccatcc agagggcttt acagtgtaca tatcaaccca tatctcattc ccttctttat
     5341 cgggttacag aaccggttta cgcagtttcg gcttagtgaa acaaaagaaa tcaccaatcc
     5401 gtatgccatg cgtttatacg aatccctgtg tcagtatcgt aagccggatg gctcaggcat
     5461 cgtgtctctg aaaatcgact ggatcataga gcgttaccag ctgcctcaaa gttaccagcg
     5521 tatgcctgac ttccgccgcc gcttcctgca ggtctgtgtt aatgagatca acagcagaac
     5581 tccaatgcgc ctctcataca ttgagaaaaa gaaaggccgc cagacgactc atatcgtatt
     5641 ttccttccgc gatatcactt ccatgacgac aggatagtct gagggttatc tgtcacagat
     5701 ttgagggtgg ttcgtcacat ttgttctgac ctactgaggg taatttgtca cagttttgct
     5761 gtttccttca gcctgcatgg attttctcat actttttgaa ctgtaatttt taaggaagcc
     5821 aaatttgagg gcagtttgtc acagttgatt tccttctctt tcccttcgtc atgtgacctg
     5881 atatcggggg ttagttcgtc atcattgatg agggttgatt atcacagttt attactctga
     5941 attggctatc cgcgtgtgta cctctacctg gagtttttcc cacggtggat atttcttctt
     6001 gcgctgagcg taagagctat ctgacagaac agttcttctt tgcttcctcg ccagttcgct
     6061 cgctatgctc ggttacacgg ctgcggcgag cgctagtgat aataagtgac tgaggtatgt
     6121 gctcttctta tctccttttg tagtgttgct cttattttaa acaactttgc ggttttttga
     6181 tgactttgcg attttgttgt tgctttgcag taaattgcaa gatttaataa aaaaacgcaa
     6241 agcaatgatt aaaggatgtt cagaatgaaa ctcatggaaa cacttaacca gtgcataaac
     6301 gctggtcatg aaatgacgaa ggctatcgcc attgcacagt ttaatgatga cagcccggaa
     6361 gcgaggaaaa taacccggcg ctggagaata ggtgaagcag cggatttagt tggggtttct
     6421 tctcaggcta tcagagatgc cgagaaagca gggcgactac cgcacccgga tatggaaatt
     6481 cgaggacggg ttgagcaacg tgttggttat acaattgaac aaattaatca tatgcgtgat
     6541 gtgtttggta cgcgattgcg acgtgctgaa gatgtatttc caccggtgat cggggttgct
     6601 gcccataaag gtggcgttta caaaacctca gtttctgttc atcttgctca ggatctggct
     6661 ctgaaggggc tacgtgtttt gctcgtggaa ggtaacgacc cccagggaac agcctcaatg
     6721 tatcacggat gggtaccaga tcttcatatt catgcagaag atactctcct gcctttctat
     6781 cttggggaaa aggacgatgt cacttatgca ataaagccca cttgctggcc ggggcttgac
     6841 attattcctt cctgtctggc tctgcaccgt attgaaactg agttaatggg caaatttgat
     6901 gaaggtaaac tgcccaccga tccacacctg atgctccgac tggccattga aactgttgct
     6961 catgactatg atgtcatagt tattgacagc gcgcctaacc tgggtatcgg cacgattaat
     7021 gtcgtatgtg ctgctgatgt gctgattgtt cccacgcctg ctgagttgtt tgactacacc
     7081 tccgcactgc agtttttcga tatgcttcgt gatctgctca agaacgttga tcttaaaggg
     7141 ttcgagcctg atgtacgtat tttgcttacc aaatacagca atagtaatgg ctctcagtcc
     7201 ccgtggatgg aggagcaaat tcgggatgcc tggggaagca tggttctaaa aaatgttgta
     7261 cgtgaaacgg atgaagttgg taaaggtcag atccggatga gaactgtttt tgaacaggcc
     7321 attgatcaac gctcttcaac tggtgcctgg agaaatgctc tttctatttg ggaacctgtc
     7381 tgcaatgaaa ttttcgatcg tctgattaaa ccacgctggg agattagata atgaagcgtg
     7441 cgcctgttat tccaaaacat acgctcaata ctcaaccggt tgaagatact tcgttatcga
     7501 caccagctgc cccgatggtg gattcgttaa ttgcgcgcgt aggagtaatg gctcgcggta
     7561 atgccattac tttgcctgta tgtggtcggg atgtgaagtt tactcttgaa gtgctccggg
     7621 gtgatagtgt tgaaaagacc tctcgggtat ggtcaggtaa tgaacgtgac caggagctgc
     7681 ttactgagga cgcactggat gatctcatcc cttcttttct actgactggt caacagacac
     7741 cggcgttcgg tcgaagagta tctggtgtca tagaaattgc cgatgggagt cgccgtcgta
     7801 aagctgctgc acttaccgaa agtgattatc gtgttctggt tggcgagctg gatgatgagc
     7861 agatggctgc attatccaga ttgggtaacg attatcgccc aacaagtgct tatgaacgtg
     7921 gtcagcgtta tgcaagccga ttgcagaatg aatttgctgg aaatatttct gcgctggctg
     7981 atgcggaaaa tatttcacgt aagattatta cccgctgtat caacaccgcc aaattgccta
     8041 aatcagttgt tgctcttttt tctcaccccg gtgaactatc tgcccggtca ggtgatgcac
     8101 ttcaaaaagc ctttacagat aaagaggaat tacttaagca gcaggcatct aaccttcatg
     8161 agcagaaaaa agctggggtg atatttgaag ctgaagaagt tatcactctt ttaacttctg
     8221 tgcttaaaac gtcatctgca tcaagaacta gtttaagctc acgacatcag tttgctcctg
     8281 gagcgacagt attgtataag ggcgataaaa tggtgcttaa cctggacagg tcgcgtgttc
     8341 caactgagtg tatagagaaa attgaggcca ttcttaagga acttgaaaag ccagcaccct
     8401 gatgcgacca cgttttagtc tacgtttatc tgtctttact taatgtcctt tgttacaggc
     8461 cagaaagcat aactggcctg aatattctct ctgggcccac tgttccactt gtatcgtcgg
     8521 tctgataatc agactggga
//
