LOCUS       sequence-336788-        8793 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Omega2 Basic cloning vector GB2.0 compatible P[acman]-derived 
            backbone. Allows for copy induction from low to high using AutoFOS 
            or similar. Requires EPI300 bacterial strain (DH10B Derived) or 
            similar. uses blue-white screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8793)
  AUTHORS   Matinyan N, Karkhanis MS, Gonzalez Y, Jain A, Saltzman A, 
            Malovannaya A, Sarrion-Perdigones A, Dierick HA, Venken KJT
  TITLE     Multiplexed drug-based selection and counterselection genetic 
            manipulations in Drosophila.
  JOURNAL   Cell Rep. 2021 Sep 14;36(11):109700. doi: 
            10.1016/j.celrep.2021.109700.
  PUBMED    34525356
REFERENCE   2  (bases 1 to 8793)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-09-14"; volume: "36(11):109700. doi"; pages: " 
            10.1016/j.celrep.2021.109700"
COMMENT     Sequence Label: P[acman]-Omega2
FEATURES             Location/Qualifiers
     source          1..8793
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(392..1363)
                     /codon_start=1
                     /gene="sopB"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopB
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGNA
                     ITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQTP
                     AFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSAYER
                     GQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSARSGD
                     ALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLSSRHQF
                     APGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     CDS             complement(1363..2538)
                     /codon_start=1
                     /gene="sopA"
                     /product="partitioning protein for the bacterial F plasmid"
                     /label=sopA
                     /translation="MFRMKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRI
                     GEAADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRA
                     EDVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPTD
                     PHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQFF
                     DMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRETDE
                     VGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     misc_feature    2864..3114
                     /gene="incC"
                     /label=incC
                     /note="incompatibility region of the bacterial F plasmid"
     CDS             complement(3117..3872)
                     /codon_start=1
                     /gene="repE"
                     /product="replication initiation protein for the bacterial 
                     F plasmid"
                     /label=repE
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVDQ
                     IRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEEDAG
                     DEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNPYAM
                     RLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINSRTPM
                     RLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     rep_origin      3963..4182
                     /label=ori2
                     /note="secondary origin of replication for the bacterial F 
                     plasmid; also known as oriS"
     rep_origin      4269..4904
                     /label=oriV
                     /note="incP origin of replication"
     promoter        5839..5941
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             5942..6601
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6008..6027)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     protein_bind    6741..6810
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     misc_feature    join(7025..7055,7056..7257)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        7291..7338
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        7392..7410
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     7392..7409
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     misc_feature    7417..7473
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(7475..7494)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7476..7494)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7501..7518)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7501..7517)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7510..7532)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(7726..7745)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(7778..8332,8333..8363)
                     /label=P element 5' end
     misc_feature    join(8639..8793,1..319)
                     /gene="sopC"
                     /label=sopC
                     /note="centromere-like partitioning region of the bacterial
                     F plasmid"
ORIGIN
        1 agactaataa tcagaccgac gatacgagtg ggaccgtggt cccagactaa taatcagacc
       61 gacgatacga gtgggaccat ggtcccagac taataatcag accgacgata cgagtgggac
      121 cgtggtccca gtctgattat cagaccgacg atacgagtgg gaccgtggtc ccagactaat
      181 aatcagaccg acgatacgag tgggaccgtg gtcccagact aataatcaga ccgacgatac
      241 gagtgggacc gtggtcccag tctgattatc agaccgacga tacaagtgga acagtgggcc
      301 cagagagaat attcaggcca gttatgcttt ctggcctgta acaaaggaca ttaagtaaag
      361 acagataaac gtagactaaa acgtggtcgc atcagggtgc tggcttttca agttccttaa
      421 gaatggcctc aattttctct atacactcag ttggaacacg cgacctgtcc aggttaagca
      481 ccattttatc gcccttatac aatactgtcg ctccaggagc aaactgatgt cgtgagctta
      541 aactagttct tgatgcagat gacgttttaa gcacagaagt taaaagagtg ataacttctt
      601 cagcttcaaa tatcacccca gcttttttct gctcatgaag gttagatgcc tgctgcttaa
      661 gtaattcctc tttatctgta aaggcttttt gaagtgcatc acctgaccgg gcagatagtt
      721 caccggggtg agaaaaaaga gcaacaactg atttaggcaa tttggcggtg ttgatacagc
      781 gggtaataat cttacgtgaa atattttccg catcagccag cgcagaaata tttccagcaa
      841 attcattctg caatcggctt gcataacgct gaccacgttc ataagcactt gttgggcgat
      901 aatcgttacc caatctggat aatgcagcca tctgctcatc atccagctcg ccaaccagaa
      961 cacgataatc actttcggta agtgcagcag ctttacgacg gcgactccca tcggcaattt
     1021 ctatgacacc agatactctt cgaccgaacg ccggtgtctg ttgaccagtc agtagaaaag
     1081 aagggatgag atcatccagt gcgtcctcag taagcagctc ctggtcacgt tcattacctg
     1141 accatacccg agaggtcttt tcaacactat caccccggag cacttcaaga gtaaacttca
     1201 catcccgacc acatacaggc aaagtaatgg cattaccgcg agccattact cctacgcgcg
     1261 caattaacga atccaccatc ggggcagctg gtgtcgataa cgaagtatct tcaaccggtt
     1321 gagtattgag cgtatgtttt ggaataacag gcgcacgctt cattatctaa tctcccagcg
     1381 tggtttaatc agacgatcga aaatttcatt gcagacaggt tcccaaatag aaagagcatt
     1441 tctccaggca ccagttgaag agcgttgatc aatggcctgt tcaaaaacag ttctcatccg
     1501 gatctgacct ttaccaactt catccgtttc acgtacaaca ttttttagaa ccatgcttcc
     1561 ccaggcatcc cgaatttgct cctccatcca cggggactga gagccattac tattgctgta
     1621 tttggtaagc aaaatacgta catcaggctc gaacccttta agatcaacgt tcttgagcag
     1681 atcacgaagc atatcgaaaa actgcagtgc ggaggtgtag tcaaacaact cagcaggcgt
     1741 gggaacaatc agcacatcag cagcacatac gacattaatc gtgccgatac ccaggttagg
     1801 cgcgctgtca ataactatga catcatagtc atgagcaaca gtttcaatgg ccagtcggag
     1861 catcaggtgt ggatcggtgg gcagtttacc ttcatcaaat ttgcccatta actcagtttc
     1921 aatacggtgc agagccagac aggaaggaat aatgtcaagc cccggccagc aagtgggctt
     1981 tattgcataa gtgacatcgt ccttttcccc aagatagaaa ggcaggagag tatcttctgc
     2041 atgaatatga agatctggta cccatccgtg atacattgag gctgttccct gggggtcgtt
     2101 accttccacg agcaaaacac gtagcccctt cagagccaga tcctgagcaa gatgaacaga
     2161 aactgaggtt ttgtaaacgc cacctttatg ggcagcaacc ccgatcaccg gtggaaatac
     2221 atcttcagca cgtcgcaatc gcgtaccaaa cacatcacgc atatgattaa tttgttcaat
     2281 tgtataacca acacgttgct caacccgtcc tcgaatttcc atatccgggt gcggtagtcg
     2341 ccctgctttc tcggcatctc tgatagcctg agaagaaacc ccaactaaat ccgctgcttc
     2401 acctattctc cagcgccggg ttattttcct cgcttccggg ctgtcatcat taaactgtgc
     2461 aatggcgata gccttcgtca tttcatgacc agcgtttatg cactggttaa gtgtttccat
     2521 gagtttcatt ctgaacatcc tttaatcatt gctttgcgtt tttttattaa atcttgcaat
     2581 ttactgcaaa gcaacaacaa aatcgcaaag tcatcaaaaa accgcaaagt tgtttaaaat
     2641 aagagcaaca ctacaaaagg agataagaag agcacatacc tcagtcactt attatcacta
     2701 gcgctcgccg cagccgtgta accgagcata gcgagcgaac tggcgaggaa gcaaagaaga
     2761 actgttctgt cagatagctc ttacgctcag cgcaagaaga aatatccacc gtgggaaaaa
     2821 ctccaggtag aggtacacac gcggatagcc aattcagagt aataaactgt gataatcaac
     2881 cctcatcaat gatgacgaac taacccccga tatcaggtca catgacgaag ggaaagagaa
     2941 ggaaatcaac tgtgacaaac tgccctcaaa tttggcttcc ttaaaaatta cagttcaaaa
     3001 agtatgagaa aatccatgca ggctgaagga aacagcaaaa ctgtgacaaa ttaccctcag
     3061 taggtcagaa caaatgtgac gaaccaccct caaatctgtg acagataacc ctcagactat
     3121 cctgtcgtca tggaagtgat atcgcggaag gaaaatacga tatgagtcgt ctggcggcct
     3181 ttctttttct caatgtatga gaggcgcatt ggagttctgc tgttgatctc attaacacag
     3241 acctgcagga agcggcggcg gaagtcaggc atacgctggt aactttgagg cagctggtaa
     3301 cgctctatga tccagtcgat tttcagagac acgatgcctg agccatccgg cttacgatac
     3361 tgacacaggg attcgtataa acgcatggca tacggattgg tgatttcttt tgtttcacta
     3421 agccgaaact gcgtaaaccg gttctgtaac ccgataaaga agggaatgag atatgggttg
     3481 atatgtacac tgtaaagccc tctggatgga ctgtgcgcac gtttgataaa ccaaggaaaa
     3541 gattcatagc ctttttcatc gccggcatcc tcttcagggc gataaaaaac cacttccttc
     3601 cccgcgaaac tcttcaatgc ctgccgtata tccttactgg cttccgcaga ggtcaatccg
     3661 aatatttcag catatttagc aacatggatc tcgcagatac cgtcatgttc ctgtagggtg
     3721 ccatcagatt ttctgatctg gtcaacgaac agatacagca tacgtttttg atcccgggag
     3781 agactatatg ccgcctcagt gaggtcgttt gactggacga ttcgcgggct atttttacgt
     3841 ttcttgtgat tgataaccgc tgtttccgcc atgacagatc catgtgaagt gtgacaagtt
     3901 tttagattgt cacactaaat aaaaaagagt caataagcag ggataacttt gtgaaaaaac
     3961 agcttcttct gagggcaatt tgtcacaggg ttaagggcaa tttgtcacag acaggactgt
     4021 catttgaggg tgatttgtca cactgaaagg gcaatttgtc acaacacctt ctctagaacc
     4081 agcatggata aaggcctaca aggcgctcta aaaaagaaga tctaaaaact ataaaaaaaa
     4141 taattataaa aatatccccg tggataagtg gataacccca agggaagttt tttcaggcat
     4201 cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca ctcgaaattc
     4261 ccctttccga cgctcaccgg gctggttgcc ctcgccgctg ggctggcggc cgtctatggc
     4321 cctgcaaacg cgccagaaac gccgtcgaag ccgtgtgcga gacaccgcgg ccggccgccg
     4381 gcgttgtgga tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga
     4441 cacttgaggg gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg
     4501 gccggcgacg tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag
     4561 tttcccacag atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag
     4621 gggcgcgact actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga
     4681 cagatgaggg gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat
     4741 ttgcaagggt ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa
     4801 accaatattt ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac
     4861 gccgaagggg ggtgcccccc cttctcgaac cctcccggcc cgctaacgct ggcctcccat
     4921 ccccccaggg gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcaggggatc
     4981 tcgagggctt cgccctgtcg ctcgactgcg gcgagcacta ctggctgtaa aaggacagac
     5041 cacatcatgg ttctgtgttc attaggttgt tctgtccatt gctgacataa tccgctccac
     5101 ttcaacgtaa caccgcacga agatttctat tgttcctgaa ggcatattca aatcgttttc
     5161 gttaccgctt gcaggcatca tgacagaaca ctacttccta taaacgctac acaggctcct
     5221 gagattaata atgcggatct ctacgataat gggagatttt cccgactgtt tcgttcgctt
     5281 ctcagtggat aacagccagc ttctctgttt aacagacaaa aacagcatat ccactcagtt
     5341 ccacatttcc atataaaggc caaggcattt attctcagga taattgtttc agcatcgcaa
     5401 ccgcatcaga ctccggcatc gcaaactgca cccggtgccg ggcagccaca tccagcgcaa
     5461 aaaccttcgt gtagacttcc gttgaactga tggacttatg tcccatcagg ctttgcagaa
     5521 ctttcagcgg tataccggca tacagcatgt gcatcgcata ggaatggcgg aacgtatgtg
     5581 gtgtgaccgg aacagagaac gtcacaccgt cagcagcagc ggcggcaacc gcctccccaa
     5641 tccaggtcct gaccgttctg tccgtcactt cccagatccg cgctttctct gtccttcctg
     5701 tgcgacggtt acgccgctcc atgagcttat cgcgaataaa tacctgtgac ggaagatcac
     5761 ttcgcagaat aaataaatcc tggtgtccct gttgataccg ggaagccctg ggccaacttt
     5821 tggcgaaaat gagagtgatg atcggcacgt aagaggttcc aactttcacc ataatgaaat
     5881 aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta aggaagctaa
     5941 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
     6001 acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga
     6061 tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat
     6121 tcacattctt gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg
     6181 tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga
     6241 aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata
     6301 ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga
     6361 gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc accagttttg atttaaacgt
     6421 ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg
     6481 cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca
     6541 tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta
     6601 atttttttaa ggcagttatt ggtgccctta aacgcctggt tgctacgcct gaataagtga
     6661 taataagcgg atgaatggca gaaattcgat gataagctgt caaacatgat gttgaattgg
     6721 tcgacgatgt aggtcactgt ctcgaagccg cggtgcgggt gccagggcgt gcccttgggc
     6781 tccccgggcg cgtactccac ctcacccatc tggtccatca tgatgaacgg gtcgaggtgg
     6841 cggtagttga tcccggcgaa cgcgcggcgc accgggaagc cctcgccctc gaaaccgctg
     6901 ggcgcggtgg tcacggtgag cacgggacgt gcgacggcgt cggcgggtgc ggatacgcgg
     6961 ggcagcgtca gcgggttctc gacggtcacg gcgggcatgt cgatcacgat gagaatggcc
     7021 agaccatgat gaaataacat aaggtggtcc cgtcggcaag agacatccac ttaacgtatg
     7081 cttgcaataa gtgcgagtga aaggaatagt attctgagtg tcgtattgag tctgagtgag
     7141 acagcgatat gattgttgat taacccttag catgtccgtg gggtttgaat taactcataa
     7201 tattaattag acgaaattat ttttaaagtt ttatttttaa taatttgcga gtacgcataa
     7261 ctgcaggtct cagtcaggag agagacgcct gttgacaatt aatcatcggc atagtatatc
     7321 ggcatagtat aatacgacaa ggtgaggaac taaacccagg aggcagatca tgaccatgat
     7381 tacgccaagc tatttaggtg acactataga atactcaagc ttgcatgcct gcaggtcgac
     7441 tctagaggat ccccgggtac cgagctcgaa ttcgccctat agtgagtcgt attacaattc
     7501 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     7561 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     7621 cccttcccaa cagttgcgca gcctgaatgg cgaatgacgc ctgatgcggt attttctcct
     7681 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     7741 tgccgcatag cgtctctcgc ttgagacctg cagtgacgct gctgctctaa acgacgcatt
     7801 tcgtactcca aagtacgaat tttttccctc aagctcttat tttcattaaa caatgaacag
     7861 gacctaacgc acagtcacgt tattgtttac ataaatgatt ttttttacta ttcaaactta
     7921 ctctgtttgt gtactcccac tggtatagcc ttcttttatc ttttctggtt caggctctat
     7981 cactttacta ggtacggcat ctgcgttgag tcgcctcctt ttaaatgtct gaccttttgc
     8041 aggtgcagcc ttccactgcg aatcattaaa gtgggtatca caaatttggg agttttcacc
     8101 aaggctgcac ccaaggctct gctcccacaa ttttctctta atagcacact tcggcacgtg
     8161 aattaatttt actccagtca cagctttgca gcaaaatttg caatatttca ttttttttta
     8221 ttccacgtaa gggttaatgt tttcaaaaaa aaattcgtcc gcacacaacc tttcctctca
     8281 acaagcaaac gtgcactgaa tttaagtgta tacttcggta agcttcggct atcgacggga
     8341 ccaccttatg ttatttcatc atgggccaga cccacgtagt ccagactagt ggatctcgac
     8401 agcgacacac ttgcatcgga tgcagcccgg ttaacgtgcc ggcacggcct gggtaaccag
     8461 gtattttgtc cacataaccg tgcgcaaaat gttgtggata agcaggacac agcagcaatc
     8521 cacagcaggc atacaaccgc acaccgaggt tactccgttc tacaggttac gacgacatgt
     8581 caatacttgc ccttgacagg cattgatgga atcgtagtct cacgctgata gtctgatcga
     8641 caatacaagt gggaccgtgg tcccagaccg ataatcagac cgacaacacg agtgggatcg
     8701 tggtcccaga ctaataatca gaccgacgat acgagtggga ccgtggtccc agactaataa
     8761 tcagaccgac gatacgagtg ggaccgtggt tcc
//
