LOCUS       sequence-336793-        9670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Doxycycline inducible expression of p21.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9670)
  AUTHORS   Lukow DA, Sausville EL, Suri P, Chunduri NK, Wieland A, Leu J, Smith
            JC, Girish V, Kumar AA, Kendall J, Wang Z, Storchova Z, Sheltzer JM
  TITLE     Chromosomal instability accelerates the evolution of resistance to 
            anti-cancer therapies.
  JOURNAL   Dev Cell. 2021 Sep 13;56(17):2427-2439.e4. doi: 
            10.1016/j.devcel.2021.07.009. Epub 2021 Aug 4.
  PUBMED    34352222
REFERENCE   2  (bases 1 to 9670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2021.07.009"; journalName: "Dev Cell"; date: 
            "2021-09-13- 13"; volume: "56"; issue: "17"; pages: "2427-2439.e4"
COMMENT     Sequence Label: pLVX-TetOne-Puro-p21
FEATURES             Location/Qualifiers
     source          1..9670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    2187..2321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2514..3008)
                     /codon_start=1
                     /gene="Homo sapiens CDKN1A"
                     /product="human cyclin-dependent kinase inhibitor 1A"
                     /label=p21
                     /note="mediates p53-dependent arrest in the G1 phase of the
                     cell cycle"
                     /translation="MSEPAGDVRQNPCGSKACRRLFGPVDSEQLRRDCDALMAGCIQEA
                     RERWNFDFVTETPLEGDFAWERVRGLGLPKLYLPTGPRRGRDELGGGRRPGTSPALLQG
                     TAEEDHVDLSLSCTLVPRSGEQAEGSPGGPGDSQGRKRRQTSMTDFYHSKRRLIFSKRK
                     P"
     promoter        complement(3018..3385)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3143..3161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3179..3197
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3215..3233
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3251..3269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3287..3305
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3323..3341
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3359..3377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3402..3912
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     3831..3849
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             3931..4677
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        4688..5017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4868..5003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5026..5625
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5026..5045)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5522..5542
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5639..6227
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5692..5712)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6110..6121)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6434..7067
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7195..7211)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7195..7211)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7208..7230)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7219..7235
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7243..7249,7250..7267,7268..7273))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7288..7309
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7426..7443)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7597..8185)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7677..7696)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8356..9147,9148..9216))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8979..8998
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9217..9321)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9369..9503
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggtttagggc
     2521 ttcctcttgg agaagatcag ccggcgtttg gagtggtaga aatctgtcat gctggtctgc
     2581 cgccgttttc gaccctgaga gtctccaggt ccacctgggg acccttcagc ctgctcccct
     2641 gagcgaggca caagggtaca agacagtgac aggtccacat ggtcttcctc tgctgtcccc
     2701 tgcagcagag caggtgaggt gccaggccgc ctgcctcctc ccaactcatc ccggcctcgc
     2761 cggggccccg tgggaaggta gagcttgggc aggccaaggc cccgcacacg ctcccaggcg
     2821 aagtcaccct ccagtggtgt ctcggtgaca aagtcgaagt tccatcgctc acgggcctcc
     2881 tggatgcagc ccgccattag cgcatcacag tcgcgtctca gctgctcgct gtccactggg
     2941 ccgaagaggc ggcggcaggc cttgctgccg catgggttct gacggacatc cccagccggt
     3001 tctgacatgc cgaattcttt acgagggtag gaagtggtac ggaaagttgg tataagacaa
     3061 aagtgttgtg gaattgaagt ttactcaaaa aatcagcact cttttatagg cgccctggtt
     3121 tacataagca aagcttatac gttctctatc actgataggg agtaaactgg atatacgttc
     3181 tctatcactg atagggagta aactgtagat acgttctcta tcactgatag ggagtaaact
     3241 ggtcatacgt tctctatcac tgatagggag taaactcctt atacgttctc tatcactgat
     3301 agggagtaaa gtctgcatac gttctctatc actgataggg agtaaactct tcatacgttc
     3361 tctatcactg atagggagta aactcgaggt gataattcca cggggttggg gttgcgcctt
     3421 ttccaaggca gccctgggtt tgcgcaggga cgcggctgct ctgggcgtgg ttccgggaaa
     3481 cgcagcggcg ccgaccctgg gtctcgcaca ttcttcacgt ccgttcgcag cgtcacccgg
     3541 atcttcgccg ctacccttgt gggccccccg gcgacgcttc ctgctccgcc cctaagtcgg
     3601 gaaggttcct tgcggttcgc ggcgtgccgg acgtgacaaa cggaagccgc acgtctcact
     3661 agtaccctcg cagacggaca gcgccaggga gcaatggcag cgcgccgacc gcgatgggct
     3721 gtggccaata gcggctgctc agcagggcgc gccgagagca gcggccggga aggggcggtg
     3781 cgggaggcgg ggtgtggggc ggtagtgtgg gccctgttcc tgcccgcgcg gtgttccgca
     3841 ttctgcaagc ctccggagcg cacgtcggca gtcggctccc tcgttgaccg aatcaccgac
     3901 ctctctcccc agggggatca tcgaattacc atgtctagac tggacaagag caaagtcata
     3961 aactctgctc tggaattact caatggagtc ggtatcgaag gcctgacgac aaggaaactc
     4021 gctcaaaagc tgggagttga gcagcctacc ctgtactggc acgtgaagaa caagcgggcc
     4081 ctgctcgatg ccctgccaat cgagatgctg gacaggcatc atacccactc ctgccccctg
     4141 gaaggcgagt catggcaaga ctttctgcgg aacaacgcca agtcataccg ctgtgctctc
     4201 ctctcacatc gcgacggggc taaagtgcat ctcggcaccc gcccaacaga gaaacagtac
     4261 gaaaccctgg aaaatcagct cgcgttcctg tgtcagcaag gcttctccct ggagaacgca
     4321 ctgtacgctc tgtccgccgt gggccacttt acactgggct gcgtattgga ggaacaggag
     4381 catcaagtag caaaagagga aagagagaca cctaccaccg attctatgcc cccacttctg
     4441 aaacaagcaa ttgagctgtt cgaccggcag ggagccgaac ctgccttcct tttcggcctg
     4501 gaactaatca tatgtggcct ggagaaacag ctaaagtgcg aaagcggcgg gccgaccgac
     4561 gcccttgacg attttgactt agacatgctc ccagccgatg cccttgacga ctttgacctt
     4621 gatatgctgc ctgctgacgc tcttgacgat tttgaccttg acatgctccc cgggtaaacg
     4681 cgcgaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     4741 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     4801 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     4861 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     4921 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     4981 gtgaggaggc ttttttggag gcctaggctt ttgcaaaacg cgaccatgac cgagtacaag
     5041 cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac cctcgccgcc
     5101 gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca catcgagcgg
     5161 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     5221 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     5281 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     5341 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     5401 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     5461 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     5521 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     5581 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcg tctggaacaa
     5641 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5701 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5761 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5821 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     5881 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     5941 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6001 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6061 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6121 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6181 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     6241 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6301 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6361 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6421 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     6481 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     6541 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     6601 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     6661 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     6721 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     6781 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     6841 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     6901 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6961 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7021 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7081 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7141 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7201 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7261 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7321 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7381 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7441 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7501 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7561 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7621 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7681 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7741 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7801 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7861 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7921 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7981 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8041 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8101 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8161 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8221 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8281 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8341 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8401 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8461 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8521 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8581 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8641 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8701 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8761 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8821 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     8881 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     8941 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9001 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9061 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9121 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9181 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9241 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9301 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9361 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9421 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9481 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     9541 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     9601 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     9661 acttagtagt
//
