LOCUS       sequence-336799-       10167 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  This Cre-ERT2 expressing construct can be used to inducibly 
            recombine loxp sites. It can be used with poly-loxP containing 
            plasmids to generate timestamp barcodes useful for linage tracing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10167)
  AUTHORS   Kim IS, Wu J, Rahme GJ, Battaglia S, Dixit A, Gaskell E, Chen H, 
            Pinello L, Bernstein BE
  TITLE     Parallel Single-Cell RNA-Seq and Genetic Recording Reveals Lineage 
            Decisions in Developing Embryoid Bodies.
  JOURNAL   Cell Rep. 2020 Oct 6;33(1):108222. doi: 
            10.1016/j.celrep.2020.108222.
  PUBMED    33027665
REFERENCE   2  (bases 1 to 10167)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2020-10-6"; volume: "33(1):108222. doi"; pages: " 
            10.1016/j.celrep.2020.108222"
COMMENT     Sequence Label: p-EF1a-CreERT2-3Xflag-T2A-eBFP2
FEATURES             Location/Qualifiers
     source          1..10167
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1178
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1169
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1240..2268
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             2281..3216
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             3226..3291
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3298..3351
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(3364..3366,3367..4080)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIKV
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLLE
                     FRTAAGITLGMDELYK"
     misc_feature    4087..4675
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4140..4160)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4558..4569)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4762..4995
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5067..5188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5104..5123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5158..5177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5228..5363
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5383..5402)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5384..5402)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5412..5429)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5412..5428)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5421..5443)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5570..6025
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5657..5676)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5867..5888
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6051..6155
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6156..6224,6225..7016)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6374..6393)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7187..7775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7676..7695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7929..7946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8063..8084
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8099..8104,8105..8122,8123..8129)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8137..8153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8142..8164
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8161..8177
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8161..8177
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8196..8216
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8198..8216
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8244..8470
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8471..8651
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8698..8823
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9316..9549
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9734..9778
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10045..10161
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gttttttctt ccatttcagg tgtcgtgagc ctggagacgc catccacgct
     1201 gttttgacct ccatagaaga caccgactct agagccacca tgtccaattt actgaccgta
     1261 caccaaaatt tgcctgcatt accggtcgat gcaacgagtg atgaggttcg caagaacctg
     1321 atggacatgt tcagggatcg ccaggcgttt tctgagcata cctggaaaat gcttctgtcc
     1381 gtttgccggt cgtgggcggc atggtgcaag ttgaataacc ggaaatggtt tcccgcagaa
     1441 cctgaagatg ttcgcgatta tcttctatat cttcaggcgc gcggtctggc agtaaaaact
     1501 atccagcaac atttgggcca gctaaacatg cttcatcgtc ggtccgggct gccacgacca
     1561 agtgacagca atgctgtttc actggttatg cggcgaatcc gaaaagaaaa cgttgatgcc
     1621 ggtgaacgtg caaaacaggc tctagcgttc gaacgcactg atttcgacca ggttcgttca
     1681 ctcatggaaa atagcgatcg ctgccaggat atacgtaatc tggcatttct ggggattgct
     1741 tataacaccc tgttacgtat agccgaaatt gccaggatca gggttaaaga tatctcacgt
     1801 actgacggtg ggagaatgtt aatccatatt ggcagaacga aaacgctggt tagcaccgca
     1861 ggtgtagaga aggcacttag cctgggggta actaaactgg tcgagcgatg gatttccgtc
     1921 tctggtgtag ctgatgatcc gaataactac ctgttttgcc gggtcagaaa aaatggtgtt
     1981 gccgcgccat ctgccaccag ccagctatca actcgcgccc tggaagggat ttttgaagca
     2041 actcatcgat tgatttacgg cgctaaggat gactctggtc agagatacct ggcctggtct
     2101 ggacacagtg cccgtgtcgg agccgcgcga gatatggccc gcgctggagt ttcaataccg
     2161 gagatcatgc aagctggtgg ctggaccaat gtaaatattg tcatgaacta tatccgtaac
     2221 ctggatagtg aaacaggggc aatggtgcgc ctgctggaag atggcgatct cgagccatct
     2281 gctggagaca tgagagctgc caacctttgg ccaagcccgc tcatgatcaa acgctctaag
     2341 aagaacagcc tggccttgtc cctgacggcc gaccagatgg tcagtgcctt gttggatgct
     2401 gagcccccca tactctattc cgagtatgat cctaccagac ccttcagtga agcttcgatg
     2461 atgggcttac tgaccaacct ggcagacagg gagctggttc acatgatcaa ctgggcgaag
     2521 agggtgccag gctttgtgga tttgaccctc catgatcagg tccaccttct tgaatgtgcc
     2581 tggctagaga tcctgatgat tggtctcgtc tggcgctcca tggagcaccc agtgaagcta
     2641 ctgtttgctc ctaacttgct cttggacagg aaccagggaa aatgtgtaga gggcatggtg
     2701 gagatcttcg acatgctgct ggctacatca tctcggttcc gcatgatgaa tctgcaggga
     2761 gaggagtttg tgtgcctcaa atctattatt ttgcttaatt ctggagtgta cacatttctg
     2821 tccagcaccc tgaagtctct ggaagagaag gaccatatcc accgagtcct ggacaagatc
     2881 acagacactt tgatccacct gatggccaag gcaggcctga ccctgcagca gcagcaccag
     2941 cggctggccc agctcctcct catcctctcc cacatcaggc acatgagtaa caaaggcatg
     3001 gagcatctgt acagcatgaa gtgcaagaac gtggtgcccc tctatgacct gctgctggag
     3061 gcggcggacg cccaccgcct acatgcgccc actagccgtg gaggggcatc cgtggaggag
     3121 acggaccaaa gccacttggc cactgcgggc tctacttcat cgcattcctt gcaaaagtat
     3181 tacatcacgg gggaggcaga gggtttccct gccacagctg gatccgacta caaagaccat
     3241 gacggtgatt ataaagatca tgacatcgac tacaaggatg acgatgacaa gagatccgag
     3301 ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc tagtacaact
     3361 agcgtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     3421 ggcgacgtaa acggccacaa gttcagcgtg aggggcgagg gcgagggcga tgccaccaac
     3481 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     3541 ctcgtgacca ccctgagcca cggcgtgcag tgcttcgccc gctaccccga ccacatgaag
     3601 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     3661 ttcaaggacg acggcaccta caagacccgc gccgaggtga agttcgaggg cgacacccta
     3721 gtgaaccgca tcgagctgaa gggcgtcgac ttcaaggagg acggcaacat cctggggcac
     3781 aagctggagt acaacttcaa cagccacaac atctatatca tggccgtcaa gcagaagaac
     3841 ggcatcaagg tgaacttcaa gatccgccac aacgtggagg acggcagcgt gcagctcgcc
     3901 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacagccac
     3961 tacctgagca cccagtccgt gctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     4021 ctgctggagt tccgcaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
     4081 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     4141 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     4201 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     4261 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     4321 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     4381 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     4441 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     4501 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     4561 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     4621 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     4681 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     4741 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     4801 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     4861 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     4921 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     4981 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5041 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5101 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5161 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5221 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5281 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5341 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     5401 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5461 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5521 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     5581 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     5641 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     5701 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     5761 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     5821 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     5881 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     5941 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6001 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6061 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6121 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6181 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6241 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6301 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6361 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6421 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6481 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6541 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     6601 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     6661 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     6721 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     6781 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     6841 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     6901 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     6961 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7021 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7081 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7141 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7201 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7261 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7321 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7381 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7441 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7501 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7561 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     7621 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     7681 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     7741 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     7801 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     7861 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     7921 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     7981 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8041 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8101 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8161 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8221 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     8281 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     8341 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     8401 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8461 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8521 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     8581 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     8641 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     8701 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     8761 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     8821 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     8881 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     8941 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9001 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9061 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9121 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     9181 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     9241 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9301 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9361 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9421 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9481 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     9541 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
     9601 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
     9661 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
     9721 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
     9781 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
     9841 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
     9901 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc
     9961 cgaaggaata gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa
    10021 cggatctcga cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc
    10081 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
    10141 aattacaaaa ttcaaaattt tatcgat
//
