LOCUS       sequence-33681-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iC
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        100..412
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for mouse U6 snRNA (Das 
                     et al., 1988)"
     primer_bind     331..351
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     primer_bind     428..444
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     429..445
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(487..520)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
       61 gcagagatcc agtttggtta gtaccgggcc cgctctagag atccgacgcg ccatctctag
      121 gcccgcgccg gccccctcgc acagacttgt gggagaagct cggctactcc cctgccccgg
      181 ttaatttgca tataatattt cctagtaact atagaggctt aatgtgcgat aaaagacaga
      241 taatctgttc tttttaatac tagctacatt ttacatgata ggcttggatt tctataagag
      301 atacaaatac taaattatta ttttaaaaaa cagcacaaaa ggaaactcac cctaactgta
      361 aagtaattgt gtgttttgag actataaata tcccttggag aaaagccttg ttaacgcgcg
      421 gtgaccctcg aggtcgacgg tatcgataag ctcgcttcac gagattccag caggtcgagg
      481 gacctaataa cttcgtatag catacattat acgaagttat attaagggtt ccaagcttaa
      541 gcggccggcc gctgaaagac cccacctgta ggtttggcaa gctagctgca gtaacgccat
      601 tttgcaaggc atggaaaaat accaaaccaa gaatagagaa gttcagatca agggcgggta
      661 catgaaaata gctaacgttg ggccaaacag gatatctgcg gtgagcagtt tcggccccgg
      721 cccggggcca agaacagatg gtcaccgcag tttcggcccc ggcccgaggc caagaacaga
      781 tggtccccag atatggccca accctcagca gtttcttaag acccatcaga tgtttccagg
      841 ctcccccaag gacctgaaat gaccctgcgc cttatttgaa ttaaccaatc agcctgcttc
      901 tcgcttctgt tcgcgcgctt ctgcttcccg agctctataa aagagctcac
//
