LOCUS       sequence-33682-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iC
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    128..680
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    130..697
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(290..309)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     519..536
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccatggtt
      121 gtggccatat tatcatcgtg tttttcaaag gaaaaccacg tccccgtggt tcggggggcc
      181 tagacgtttt tttaacctcg actaaacaca tgtaaagcat gtgcaccgag gccccagatc
      241 agatcccata caatggggta ccttctgggc atccttcagc cccttgttga atacgcttga
      301 ggagagccat ttgactcttt ccacaactat ccaactcaca acgtggcact ggggttgtgc
      361 cgcctttgca ggtgtatctt atacacgtgg cttttggccg cagaggcacc tgtcgccagg
      421 tggggggttc cgctgcctgc aaagggtcgc tacagacgtt gtttgtcttc aagaagcttc
      481 cagaggaact gcttccttca cgacattcaa cagaccttgc attcctttgg cgagagggga
      541 aagaccccta ggaatgctcg tcaagaagac agggccaggt ttccgggccc tcacattgcc
      601 aaaagacggc aatatggtgg aaaataacat atagacaaac gcacaccggc cttattccaa
      661 gcggcttcgg ccagtaacgt tagggggggg ggagggaga
//
