LOCUS       sequence-33685-e         934 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 934)
  AUTHORS   Weber K, Bartsch U, Stocking C, Fehse B
  TITLE     A multicolor panel of novel lentiviral ""gene ontology"" (LeGO) 
            vectors for functional gene analysis.
  JOURNAL   Mol Ther. 2008 Apr . 16(4):698-706.
  PUBMED    18362927
REFERENCE   2  (bases 1 to 934)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Ther. 
            2008 Apr . 16(4):698-706."
COMMENT     Sequence Label: LeGO-iCer
FEATURES             Location/Qualifiers
     source          1..934
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(67..100)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    156..744
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(209..229)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(627..638)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(747..763)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(748..764)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tggacgagct gtacaagtaa agcggccggc cgccagcaca gtggtcgaaa ttcgtcgagg
       61 gacctaataa cttcgtatag catacattat acgaagttat acatgtttaa gggttccggt
      121 tccactaggt acaattcgat atcaagctta tcgataatca acctctggat tacaaaattt
      181 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
      241 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
      301 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
      361 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
      421 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
      481 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
      541 tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc
      601 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
      661 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
      721 tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgatcgaga cctagaaaaa
      781 catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc ctggctagaa
      841 gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt aagaccaatg
      901 acttacaagg cagctgtaga tcttagccac tttt
//
