LOCUS       sequence-336851-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 E61V mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_E61V_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(83..103)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        88..417
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      268..403
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        465..512
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             531..905
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1035..1156
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1072..1091)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1126..1145
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1205..1221)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1205..1221)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1218..1240)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1229..1245
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1253..1259,1260..1277,1278..1283))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1298..1319
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1436..1453)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1607..2195)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1687..1706)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2366..3157,3158..3226))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2989..3008
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3227..3331)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3406..3425)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3597..3976
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3978..4176
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4131..4151
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             4194..4374
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4421..4546
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5039..5272
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5457..5501
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5799..5916
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6052..7230
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6283..7221
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7178..7198
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     7247..7266
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7247..7265
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             7329..8351
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7329..8351
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8361..8417
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(9971..10004)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             10029..10209
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(10235..10252)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10241..10465
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(10511..10865,1..74)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10598..10617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10808..10829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
       61 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
      121 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
      181 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
      241 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
      301 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
      361 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
      421 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
      481 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
      541 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
      601 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
      661 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
      721 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
      781 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
      841 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
      901 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
      961 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     1021 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     1081 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     1141 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     1201 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     1261 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     1321 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     1381 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     1441 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     1501 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     1561 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     1621 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     1681 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     1741 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     1801 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     1861 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     1921 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     1981 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     2041 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     2101 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     2161 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     2221 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     2281 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     2341 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     2401 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     2461 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     2521 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     2581 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     2641 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     2701 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     2761 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     2821 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     2881 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     2941 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     3001 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     3061 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     3121 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     3181 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     3241 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     3301 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     3361 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
     3421 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
     3481 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
     3541 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
     3601 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
     3661 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     3721 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     3781 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     3841 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     3901 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     3961 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     4021 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     4081 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     4141 cggtaggcgt gtacggtggg aggtctatat aagcagcgcg ttttgcctgt actgggtctc
     4201 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4261 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4321 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     4381 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     4441 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     4501 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     4561 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     4621 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     4681 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     4741 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     4801 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     4861 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     4921 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     4981 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     5041 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     5101 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     5161 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     5221 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     5281 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     5341 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     5401 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     5461 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     5521 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     5581 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     5641 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     5701 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatcg gcactgcgtg
     5761 cgccaattct gcagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
     5821 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     5881 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
     5941 gatccagttt ggttaattaa tcgataagct ttgcaaagat ggataaagtt ttaaacagag
     6001 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     6061 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     6121 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     6181 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     6241 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     6301 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     6361 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     6421 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     6481 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     6541 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     6601 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     6661 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     6721 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     6781 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     6841 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     6901 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     6961 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     7021 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     7081 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     7141 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     7201 agtttttttc ttccatttca ggtgtcgtga ggaattagct tggtactaat acgactcact
     7261 atagggagac ccaagctggc taggtaagct tgatatcgaa ttcctgcagc ccgggggatc
     7321 ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt cgagaagttt ctgatcgaaa
     7381 agttcgacag cgtctccgac ctgatgcagc tctcggaggg cgaagaatct cgtgctttca
     7441 gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa tagctgcgcc gatggtttct
     7501 acaaagatcg ttatgtttat cggcactttg catcggccgc gctcccgatt ccggaagtgc
     7561 ttgacattgg ggaattcagc gagagcctga cctattgcat ctcccgccgt gcacagggtg
     7621 tcacgttgca agacctgcct gaaaccgaac tgcccgctgt tctgcagccg gtcgcggagg
     7681 ccatggatgc gatcgctgcg gccgatctta gccagacgag cgggttcggc ccattcggac
     7741 cgcaaggaat cggtcaatac actacatggc gtgatttcat atgcgcgatt gctgatcccc
     7801 atgtgtatca ctggcaaact gtgatggacg acaccgtcag tgcgtccgtc gcgcaggctc
     7861 tcgatgagct gatgctttgg gccgaggact gccccgaagt ccggcacctc gtgcacgcgg
     7921 atttcggctc caacaatgtc ctgacggaca atggccgcat aacagcggtc attgactgga
     7981 gcgaggcgat gttcggggat tcccaatacg aggtcgccaa catcttcttc tggaggccgt
     8041 ggttggcttg tatggagcag cagacgcgct acttcgagcg gaggcatccg gagcttgcag
     8101 gatcgccgcg gctccgggcg tatatgctcc gcattggtct tgaccaactc tatcagagct
     8161 tggttgacgg caatttcgat gatgcagctt gggcgcaggg tcgatgcgac gcaatcgtcc
     8221 gatccggagc cgggactgtc gggcgtacac aaatcgcccg cagaagcgcg gccgtctgga
     8281 ccgatggctg tgtagaagta ctcgccgata gtggaaaccg acgccccagc actcgtccga
     8341 gggcaaagga aggatccggc gcaacaaact tctctctgct gaaacaagcc ggagatgtcg
     8401 aagagaatcc tggaccgatg cgtatccccg tagatgccag cacgagccgc cgcttcacgc
     8461 cgccttccac cgcgctgagc ccaggcaaga tgagcgaggc gttgccgctg ggcgccccgg
     8521 acgccggcgc tgccctggcc ggcaagctga ggagcggcga ccgcagcatg gtggaggtgc
     8581 tggccgacca cccgggcgtg ctggtgcgca ccgacagccc caacttcctc tgctccgtgc
     8641 tgcctacgca ctggcgctgc aacaagaccc tgcccatcgc tttcaaggtg gtggccctag
     8701 gggatgttcc agatggcact ctggtcactg tgatggctgg caatgatgaa aactactcgg
     8761 ctgagctgag aaatgctacc gcagccatga agaaccaggt tgcaagattt aatgacctca
     8821 ggtttgtcgg tcgaagtgga agagggaaaa gcttcactct gaccatcact gtcttcacaa
     8881 acccaccgca agtcgccacc taccacagag ccatcaaaat cacagtggat gggccccgag
     8941 aacctcgaag acatcggcag aaactagatg atcagaccaa gcccgggagc ttgtcctttt
     9001 ccgagcggct cagtgaactg gagcagctgc ggcgcacagc catgagggtc agcccacacc
     9061 acccagcccc cacgcccaac cctcgtgcct ccctgaacca ctccactgcc tttaaccctc
     9121 agcctcagag tcagatgcag gatacaaggc agatccaacc atccccaccg tggtcctacg
     9181 atcagtccta ccaatacctg ggatccattg cctctccttc tgtgcaccca gcaacgccca
     9241 tttcacctgg acgtgccagc ggcatgacaa ccctctctgc agaactttcc agtcgactct
     9301 caacggcacc cgacctgaca gcgttcagcg acccgcgcca gttccccgcg ctgccctcca
     9361 tctccgaccc ccgcatgcac tatccaggcg ccttcaccta ctccccgacg ccggtcacct
     9421 cgggcatcgg catcggcatg tcggccatgg gctcggccac gcgctaccac acctacctgc
     9481 cgccgcccta ccccggctcg tcgcaagcgc agggaggccc gttccaagcc agctcgccct
     9541 cctaccacct gtactacggc gcctcggccg gctcctacca gttctccatg gtgggcggcg
     9601 agcgctcgcc gccgcgcatc ctgccgccct gcaccaacgc ctccaccggc tccgcgctgc
     9661 tcaaccccag cctcccgaac cagagcgacg tggtggaggc cgagggcagc cacagcaact
     9721 cccccaccaa catggcgccc tccgcgcgcc tggaggaggc cgtgtggagg ccctactgac
     9781 tagctaagtt agaactattt cctggctgtt acgcgagtta ggggtgacta ctgacgggct
     9841 tgttaaacag tgaaccgccc gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga
     9901 ccccatgcat cgaacaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     9961 acaagatatc ataacttcgt atagcataca ttatacgaag ttatcgtcta gctggtccgg
    10021 actgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10081 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10141 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
    10201 tctctagcag ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
    10261 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
    10321 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
    10381 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
    10441 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg
    10501 gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
    10561 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
    10621 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
    10681 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
    10741 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
    10801 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
    10861 tttga
//
