LOCUS       sequence-336853-       10865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Overexpression of RUNX1 D99Y mutant protein..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ozturk K, Panwala R, Sheen J, Ford K, Jayne N, Portell A, Zhang DE, 
            Hutter S, Haferlach T, Ideker T, Mali P, Carter H
  TITLE     Interface-guided phenotyping of coding variants in the transcription
            factor RUNX1.
  JOURNAL   Cell Rep. 2024 Jul 4;43(7):114436. doi: 
            10.1016/j.celrep.2024.114436.
  PUBMED    38968069
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2024-07-4"; volume: "43(7):114436. doi"; pages: " 
            10.1016/j.celrep.2024.114436"
COMMENT     Sequence Label: EF1A_Hygro_P2A_RUNX1_D99Y_Barcode
FEATURES             Location/Qualifiers
     source          1..10865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(187..775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(267..286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(946..1737,1738..1806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1569..1588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1807..1911)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1986..2005)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        2177..2556
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2558..2756
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2711..2731
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             2774..2954
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3001..3126
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3619..3852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4037..4081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4379..4496
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4632..5810
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4863..5801
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5758..5778
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     5827..5846
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5827..5845
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             5909..6931
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5909..6931
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6941..6997
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     protein_bind    complement(8551..8584)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             8609..8789
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8815..8832)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8821..9045
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      9091..9519
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9178..9197)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9388..9409
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9528..9548)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9533..9862
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9713..9848
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9910..9957
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9976..10350
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10480..10601
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10517..10536)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10571..10590
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10650..10666)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10650..10666)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10663..10685)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10674..10690
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10698..10704,10705..10722,10723..10728))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10743..10764
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
       61 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      121 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      181 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      241 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      301 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      361 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      421 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      481 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      541 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      601 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      661 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      721 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
      781 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
      841 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      901 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      961 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1021 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1081 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1141 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1201 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1261 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1321 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1381 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1441 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1501 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1561 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1621 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1681 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1741 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1801 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1861 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1921 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     1981 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     2041 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     2101 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     2161 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     2221 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     2281 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     2341 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     2401 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     2461 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     2521 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     2581 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     2641 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     2701 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     2761 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2821 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2881 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2941 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     3001 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     3061 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     3121 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     3181 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     3241 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     3301 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     3361 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     3421 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     3481 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     3541 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     3601 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     3661 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     3721 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     3781 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     3841 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     3901 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     3961 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     4021 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     4081 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     4141 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     4201 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     4261 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     4321 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     4381 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     4441 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     4501 tttattacag ggacagcaga gatccagttt ggttaattaa tcgataagct ttgcaaagat
     4561 ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag gtcttgaaag
     4621 gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg
     4681 agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa
     4741 actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt
     4801 atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac
     4861 aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat ggcccttgcg
     4921 tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag cttcgggttg
     4981 gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct cgtgcttgag
     5041 ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct
     5101 gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct gctgcgacgc
     5161 tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact ggtatttcgg
     5221 tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc
     5281 ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct ggccggcctg
     5341 ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca aggctggccc
     5401 ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct gcagggagct
     5461 caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca caaaggaaaa
     5521 gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca
     5581 ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg ggggaggggt
     5641 tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg ccagcttggc
     5701 acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg ttcattctca
     5761 agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga ggaattagct
     5821 tggtactaat acgactcact atagggagac ccaagctggc taggtaagct tgatatcgaa
     5881 ttcctgcagc ccgggggatc ccgccaccat gaaaaagcct gaactcaccg cgacgtctgt
     5941 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     6001 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     6061 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     6121 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     6181 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     6241 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     6301 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     6361 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     6421 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     6481 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     6541 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     6601 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     6661 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     6721 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     6781 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     6841 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     6901 acgccccagc actcgtccga gggcaaagga aggatccggc gcaacaaact tctctctgct
     6961 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg cgtatccccg tagatgccag
     7021 cacgagccgc cgcttcacgc cgccttccac cgcgctgagc ccaggcaaga tgagcgaggc
     7081 gttgccgctg ggcgccccgg acgccggcgc tgccctggcc ggcaagctga ggagcggcga
     7141 ccgcagcatg gtggaggtgc tggccgacca cccgggcgag ctggtgcgca ccgacagccc
     7201 caacttcctc tgctccgtgc tgcctacgca ctggcgctgc aacaagaccc tgcccatcgc
     7261 tttcaaggtg gtggccctag gggatgttcc atatggcact ctggtcactg tgatggctgg
     7321 caatgatgaa aactactcgg ctgagctgag aaatgctacc gcagccatga agaaccaggt
     7381 tgcaagattt aatgacctca ggtttgtcgg tcgaagtgga agagggaaaa gcttcactct
     7441 gaccatcact gtcttcacaa acccaccgca agtcgccacc taccacagag ccatcaaaat
     7501 cacagtggat gggccccgag aacctcgaag acatcggcag aaactagatg atcagaccaa
     7561 gcccgggagc ttgtcctttt ccgagcggct cagtgaactg gagcagctgc ggcgcacagc
     7621 catgagggtc agcccacacc acccagcccc cacgcccaac cctcgtgcct ccctgaacca
     7681 ctccactgcc tttaaccctc agcctcagag tcagatgcag gatacaaggc agatccaacc
     7741 atccccaccg tggtcctacg atcagtccta ccaatacctg ggatccattg cctctccttc
     7801 tgtgcaccca gcaacgccca tttcacctgg acgtgccagc ggcatgacaa ccctctctgc
     7861 agaactttcc agtcgactct caacggcacc cgacctgaca gcgttcagcg acccgcgcca
     7921 gttccccgcg ctgccctcca tctccgaccc ccgcatgcac tatccaggcg ccttcaccta
     7981 ctccccgacg ccggtcacct cgggcatcgg catcggcatg tcggccatgg gctcggccac
     8041 gcgctaccac acctacctgc cgccgcccta ccccggctcg tcgcaagcgc agggaggccc
     8101 gttccaagcc agctcgccct cctaccacct gtactacggc gcctcggccg gctcctacca
     8161 gttctccatg gtgggcggcg agcgctcgcc gccgcgcatc ctgccgccct gcaccaacgc
     8221 ctccaccggc tccgcgctgc tcaaccccag cctcccgaac cagagcgacg tggtggaggc
     8281 cgagggcagc cacagcaact cccccaccaa catggcgccc tccgcgcgcc tggaggaggc
     8341 cgtgtggagg ccctactgac tagctaagtt agaactattt cctggctgtt acgcgggttc
     8401 ggaatcgcta ctgacgggct tgttaaacag tgaaccgccc gccccacgac ccgcagcgcc
     8461 cgaccgaaag gagcgcacga ccccatgcat cgaacaaaag aaaagggggg actggaaggg
     8521 ctaattcact cccaacgaag acaagatatc ataacttcgt atagcataca ttatacgaag
     8581 ttatcgtcta gctggtccgg actgtactgg gtctctctgg ttagaccaga tctgagcctg
     8641 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     8701 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     8761 cttttagtca gtgtggaaaa tctctagcag ggcccgttta aacccgctga tcagcctcga
     8821 ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc
     8881 tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc
     8941 tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt
     9001 gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa
     9061 gaaccagctg gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg
     9121 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     9181 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     9241 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     9301 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     9361 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     9421 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     9481 taaaaaatga gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca
     9541 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9601 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     9661 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     9721 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     9781 tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag gaggcttttt
     9841 tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt tcggatctga
     9901 tcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     9961 gtgaggaact aaaccatggc caagttgacc agtgccgttc cggtgctcac cgcgcgcgac
    10021 gtcgccggag cggtcgagtt ctggaccgac cggctcgggt tctcccggga cttcgtggag
    10081 gacgacttcg ccggtgtggt ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac
    10141 caggtggtgc cggacaacac cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac
    10201 gccgagtggt cggaggtcgt gtccacgaac ttccgggacg cctccgggcc ggccatgacc
    10261 gagatcggcg agcagccgtg ggggcgggag ttcgccctgc gcgacccggc cggcaactgc
    10321 gtgcacttcg tggccgagga gcaggactga cacgtgctac gagatttcga ttccaccgcc
    10381 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
    10441 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
    10501 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
    10561 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
    10621 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
    10681 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
    10741 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10801 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
    10861 attgg
//
